The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice.
N6-Methyladenosine (m6A) RNA methylation plays important roles during development in different species. However, knowledge of m6A RNA methylation in monocots remains limited. In this study, we reported that OsFIP and OsMTA2 are the components of m6A RNA methyltransferase complex in rice and uncovere...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-05-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1008120 |
| _version_ | 1818581855500238848 |
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| author | Fan Zhang Yu-Chan Zhang Jian-You Liao Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Mei Bai Hong Wu Yue-Qin Chen |
| author_facet | Fan Zhang Yu-Chan Zhang Jian-You Liao Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Mei Bai Hong Wu Yue-Qin Chen |
| author_sort | Fan Zhang |
| collection | DOAJ |
| description | N6-Methyladenosine (m6A) RNA methylation plays important roles during development in different species. However, knowledge of m6A RNA methylation in monocots remains limited. In this study, we reported that OsFIP and OsMTA2 are the components of m6A RNA methyltransferase complex in rice and uncovered a previously unknown function of m6A RNA methylation in regulation of plant sporogenesis. Importantly, OsFIP is essential for rice male gametogenesis. Knocking out of OsFIP results in early degeneration of microspores at the vacuolated pollen stage and simultaneously causes abnormal meiosis in prophase I. We further analyzed the profile of rice m6A modification during sporogenesis in both WT and OsFIP loss-of-function plants, and identified a rice panicle specific m6A modification motif "UGWAMH". Interestingly, we found that OsFIP directly mediates the m6A methylation of a set of threonine protease and NTPase mRNAs and is essential for their expression and/or splicing, which in turn regulates the progress of sporogenesis. Our findings revealed for the first time that OsFIP plays an indispensable role in plant early sporogenesis. This study also provides evidence for the different functions of the m6A RNA methyltransferase complex between rice and Arabidopsis. |
| first_indexed | 2024-12-16T07:40:07Z |
| format | Article |
| id | doaj.art-e87d47b242434c41b58b6d3ac44af061 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-16T07:40:07Z |
| publishDate | 2019-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-e87d47b242434c41b58b6d3ac44af0612022-12-21T22:39:06ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-05-01155e100812010.1371/journal.pgen.1008120The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice.Fan ZhangYu-Chan ZhangJian-You LiaoYang YuYan-Fei ZhouYan-Zhao FengYu-Wei YangMeng-Qi LeiMei BaiHong WuYue-Qin ChenN6-Methyladenosine (m6A) RNA methylation plays important roles during development in different species. However, knowledge of m6A RNA methylation in monocots remains limited. In this study, we reported that OsFIP and OsMTA2 are the components of m6A RNA methyltransferase complex in rice and uncovered a previously unknown function of m6A RNA methylation in regulation of plant sporogenesis. Importantly, OsFIP is essential for rice male gametogenesis. Knocking out of OsFIP results in early degeneration of microspores at the vacuolated pollen stage and simultaneously causes abnormal meiosis in prophase I. We further analyzed the profile of rice m6A modification during sporogenesis in both WT and OsFIP loss-of-function plants, and identified a rice panicle specific m6A modification motif "UGWAMH". Interestingly, we found that OsFIP directly mediates the m6A methylation of a set of threonine protease and NTPase mRNAs and is essential for their expression and/or splicing, which in turn regulates the progress of sporogenesis. Our findings revealed for the first time that OsFIP plays an indispensable role in plant early sporogenesis. This study also provides evidence for the different functions of the m6A RNA methyltransferase complex between rice and Arabidopsis.https://doi.org/10.1371/journal.pgen.1008120 |
| spellingShingle | Fan Zhang Yu-Chan Zhang Jian-You Liao Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Mei Bai Hong Wu Yue-Qin Chen The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. PLoS Genetics |
| title | The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. |
| title_full | The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. |
| title_fullStr | The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. |
| title_full_unstemmed | The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. |
| title_short | The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice. |
| title_sort | subunit of rna n6 methyladenosine methyltransferase osfip regulates early degeneration of microspores in rice |
| url | https://doi.org/10.1371/journal.pgen.1008120 |
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