Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study
This study aimed to verify the role of <i>TGFB1</i> variants (c.–1638G>A, c.–1347C>T, c.29C>T, and c.74G>C) in HPV infection susceptibility and cervical lesions development, and their impact on TGFB1 cervical and plasma levels. <i>TGFB1</i> genotypes were assessed...
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| Language: | English |
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/1/84 |
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| author | Kleber Paiva Trugilo Guilherme Cesar Martelossi Cebinelli Érica Romão Pereira Nádia Calvo Martins Okuyama Fernando Cezar-dos-Santos Eliza Pizarro Castilha Tamires Flauzino Valéria Bumiller-Bini Hoch Maria Angelica Ehara Watanabe Roberta Losi Guembarovski Karen Brajão de Oliveira |
| author_facet | Kleber Paiva Trugilo Guilherme Cesar Martelossi Cebinelli Érica Romão Pereira Nádia Calvo Martins Okuyama Fernando Cezar-dos-Santos Eliza Pizarro Castilha Tamires Flauzino Valéria Bumiller-Bini Hoch Maria Angelica Ehara Watanabe Roberta Losi Guembarovski Karen Brajão de Oliveira |
| author_sort | Kleber Paiva Trugilo |
| collection | DOAJ |
| description | This study aimed to verify the role of <i>TGFB1</i> variants (c.–1638G>A, c.–1347C>T, c.29C>T, and c.74G>C) in HPV infection susceptibility and cervical lesions development, and their impact on TGFB1 cervical and plasma levels. <i>TGFB1</i> genotypes were assessed with PCR-RFLP and haplotypes were inferred for 190 HPV-uninfected and 161 HPV-infected women. TGFB1 levels were determined with immunofluorimetric assay. Case-control analyses were performed with logistic regression adjusted for possible confounders. Women carrying –1347TT or –1347CT+TT as well as those with 29CT, 29CC, or 29CT+CC were more likely to have HPV than –1347CC and 29TT carriers, respectively. Regarding haplotypes, the most frequent were *4 (GCTG) and *3 (GTCG). Women *4/*4 were less likely to have HPV than those with no *4 copy. Comparing the inheritance of *3 and *4, carriers of *3/*4 or *3/*3 were more susceptible to HPV than *4/*4. The TGFB1 plasma and cervical levels were higher in the infected patients. Plasma levels were also higher in infected women with low-grade lesions. HPV-infected patients carrying *3/Other and *3/Other+*3/*3 presented lower TGFB1 plasma levels than those with no copy of *3. TGFB1 variants could contribute to the comprehension of the TGFB1 role in HPV-caused cervical disease. |
| first_indexed | 2024-03-11T10:06:22Z |
| format | Article |
| id | doaj.art-e884887ba40b4be59dbf79a303d93e4b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T10:06:22Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e884887ba40b4be59dbf79a303d93e4b2023-11-16T15:05:52ZengMDPI AGCells2073-44092022-12-011218410.3390/cells12010084Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control StudyKleber Paiva Trugilo0Guilherme Cesar Martelossi Cebinelli1Érica Romão Pereira2Nádia Calvo Martins Okuyama3Fernando Cezar-dos-Santos4Eliza Pizarro Castilha5Tamires Flauzino6Valéria Bumiller-Bini Hoch7Maria Angelica Ehara Watanabe8Roberta Losi Guembarovski9Karen Brajão de Oliveira10Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Research in Applied Immunology, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Human Molecular Genetics, Department of Genetics, Federal University of Paraná, Curitiba 80060-000, PR, BrazilLaboratory of Studies and Applications of DNA Polymorphisms and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Mutagenesis and Oncogenetics, Department of Biological Sciences, State University of Londrina, Londrina 86057-970, PR, BrazilLaboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, BrazilThis study aimed to verify the role of <i>TGFB1</i> variants (c.–1638G>A, c.–1347C>T, c.29C>T, and c.74G>C) in HPV infection susceptibility and cervical lesions development, and their impact on TGFB1 cervical and plasma levels. <i>TGFB1</i> genotypes were assessed with PCR-RFLP and haplotypes were inferred for 190 HPV-uninfected and 161 HPV-infected women. TGFB1 levels were determined with immunofluorimetric assay. Case-control analyses were performed with logistic regression adjusted for possible confounders. Women carrying –1347TT or –1347CT+TT as well as those with 29CT, 29CC, or 29CT+CC were more likely to have HPV than –1347CC and 29TT carriers, respectively. Regarding haplotypes, the most frequent were *4 (GCTG) and *3 (GTCG). Women *4/*4 were less likely to have HPV than those with no *4 copy. Comparing the inheritance of *3 and *4, carriers of *3/*4 or *3/*3 were more susceptible to HPV than *4/*4. The TGFB1 plasma and cervical levels were higher in the infected patients. Plasma levels were also higher in infected women with low-grade lesions. HPV-infected patients carrying *3/Other and *3/Other+*3/*3 presented lower TGFB1 plasma levels than those with no copy of *3. TGFB1 variants could contribute to the comprehension of the TGFB1 role in HPV-caused cervical disease.https://www.mdpi.com/2073-4409/12/1/84polymorphismrs1800468rs1800469rs1800470rs1800471 |
| spellingShingle | Kleber Paiva Trugilo Guilherme Cesar Martelossi Cebinelli Érica Romão Pereira Nádia Calvo Martins Okuyama Fernando Cezar-dos-Santos Eliza Pizarro Castilha Tamires Flauzino Valéria Bumiller-Bini Hoch Maria Angelica Ehara Watanabe Roberta Losi Guembarovski Karen Brajão de Oliveira Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study Cells polymorphism rs1800468 rs1800469 rs1800470 rs1800471 |
| title | Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study |
| title_full | Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study |
| title_fullStr | Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study |
| title_full_unstemmed | Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study |
| title_short | Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study |
| title_sort | haplotype structures and protein levels of tgfb1 in hpv infection and cervical lesion a case control study |
| topic | polymorphism rs1800468 rs1800469 rs1800470 rs1800471 |
| url | https://www.mdpi.com/2073-4409/12/1/84 |
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