SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485
Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects h...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12895 |
| _version_ | 1797821948857679872 |
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| author | Jin Young Sung Seul Gi Kim Du Hyong Cho Jae‐Ryong Kim Hyoung Chul Choi |
| author_facet | Jin Young Sung Seul Gi Kim Du Hyong Cho Jae‐Ryong Kim Hyoung Chul Choi |
| author_sort | Jin Young Sung |
| collection | DOAJ |
| description | Aging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects have not been well characterized. To determine the nature of the interaction between SIRT1 and AMPK in VSMC senescence, we investigated the effects of SRT1720 on its downstream targets of SIRT1 and the phosphorylation of AMPKα at Ser485. During Adriamycin‐induced VSMC senescence, SRT1720 increased the activity of SIRT1 and AMPKα phosphorylation at Ser485 via the cAMP–protein kinase A (PKA) pathway. Telomere length and telomerase reverse transcriptase expression were increased by SIRT1 activation with SRT1720. Taken together, these data show that activation of the SIRT1/cAMP–PKA/p‐AMPKα (Ser485) pathway may be an effective antisenescence mechanism for VSMCs. |
| first_indexed | 2024-03-13T10:00:16Z |
| format | Article |
| id | doaj.art-e884b1e35a0045f3808f314f1ffac48d |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T10:00:16Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-e884b1e35a0045f3808f314f1ffac48d2023-05-23T06:51:07ZengWileyFEBS Open Bio2211-54632020-07-011071316132510.1002/2211-5463.12895SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485Jin Young Sung0Seul Gi Kim1Du Hyong Cho2Jae‐Ryong Kim3Hyoung Chul Choi4Department of Pharmacology College of Medicine Yeungnam University Daegu KoreaDepartment of Pharmacology College of Medicine Yeungnam University Daegu KoreaDepartment of Pharmacology College of Medicine Yeungnam University Daegu KoreaSmart‐aging Convergence Research Center College of Medicine Yeungnam University Daegu KoreaDepartment of Pharmacology College of Medicine Yeungnam University Daegu KoreaAging is a major risk factor for hypertension and atherosclerosis, and vascular smooth muscle cell (VSMC) senescence can promote aging‐related vascular diseases. Sirtuin‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) were previously reported to modulate vascular senescence; however, its effects have not been well characterized. To determine the nature of the interaction between SIRT1 and AMPK in VSMC senescence, we investigated the effects of SRT1720 on its downstream targets of SIRT1 and the phosphorylation of AMPKα at Ser485. During Adriamycin‐induced VSMC senescence, SRT1720 increased the activity of SIRT1 and AMPKα phosphorylation at Ser485 via the cAMP–protein kinase A (PKA) pathway. Telomere length and telomerase reverse transcriptase expression were increased by SIRT1 activation with SRT1720. Taken together, these data show that activation of the SIRT1/cAMP–PKA/p‐AMPKα (Ser485) pathway may be an effective antisenescence mechanism for VSMCs.https://doi.org/10.1002/2211-5463.12895p‐AMPK (Ser485)SIRT1SRT1720telomere lengthVSMC senescence |
| spellingShingle | Jin Young Sung Seul Gi Kim Du Hyong Cho Jae‐Ryong Kim Hyoung Chul Choi SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 FEBS Open Bio p‐AMPK (Ser485) SIRT1 SRT1720 telomere length VSMC senescence |
| title | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
| title_full | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
| title_fullStr | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
| title_full_unstemmed | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
| title_short | SRT1720‐induced activation of SIRT1 alleviates vascular smooth muscle cell senescence through PKA‐dependent phosphorylation of AMPKα at Ser485 |
| title_sort | srt1720 induced activation of sirt1 alleviates vascular smooth muscle cell senescence through pka dependent phosphorylation of ampkα at ser485 |
| topic | p‐AMPK (Ser485) SIRT1 SRT1720 telomere length VSMC senescence |
| url | https://doi.org/10.1002/2211-5463.12895 |
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