Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway
Currently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3184 |
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| author | Harishkumar Madhyastha Radha Madhyastha Eshika Chakraborty Kaushita Banerjee Kamal Shah Yuichi Nakajima Nagendra Singh Chauhan Sajitha Lulu Sudhakaran Kaoru Ohe Gothandam Kodiveri Muthukaliannan Abilash Valsala Gopalakrishnan Masugi Maruyama Nozomi Watanabe |
| author_facet | Harishkumar Madhyastha Radha Madhyastha Eshika Chakraborty Kaushita Banerjee Kamal Shah Yuichi Nakajima Nagendra Singh Chauhan Sajitha Lulu Sudhakaran Kaoru Ohe Gothandam Kodiveri Muthukaliannan Abilash Valsala Gopalakrishnan Masugi Maruyama Nozomi Watanabe |
| author_sort | Harishkumar Madhyastha |
| collection | DOAJ |
| description | Currently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface mechanism. In this study, c-phycocyanin primed silver nano hybrids (AgcPCNP) were prepared and analyzed for properties such as cytotoxicity, metal decay, nanoconjugate stability, size expansion, and antioxidant features. Fluctuations in the expression of marker genes during cell migration phenomena in in vitro wound healing scenarios were also validated. Studies revealed that physiologically relevant ionic solutions did not exhibit any adverse effects on the nanoconjugate stability. However, acidic, alkali, and ethanol solutions completely denatured the AgcPCNP conjugates. Signal transduction RT<sup>2</sup>PCR array demonstrated that genes associated with NFĸB- and PI3K-pathways were significantly (<i>p</i> < 0.5%) altered between AgcPCNP and AgNP groups. Specific inhibitors of NFĸB (Nfi) and PI3K (LY294002) pathways confirmed the involvement of NFĸB signaling axes. In vitro wound healing assay demonstrated that NFĸB pathway plays a prime role in the fibroblast cell migration. In conclusion, the present investigation revealed that surface functionalized AgcPCNP accelerated the fibroblast cell migration and can be further explored for wound healing biomedical applications. |
| first_indexed | 2024-03-11T08:44:29Z |
| format | Article |
| id | doaj.art-e8874174d56f451ba34ca423c9427a22 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:44:29Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e8874174d56f451ba34ca423c9427a222023-11-16T20:56:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244318410.3390/ijms24043184Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling PathwayHarishkumar Madhyastha0Radha Madhyastha1Eshika Chakraborty2Kaushita Banerjee3Kamal Shah4Yuichi Nakajima5Nagendra Singh Chauhan6Sajitha Lulu Sudhakaran7Kaoru Ohe8Gothandam Kodiveri Muthukaliannan9Abilash Valsala Gopalakrishnan10Masugi Maruyama11Nozomi Watanabe12Division of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, JapanDivision of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, JapanSchool of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, IndiaSchool of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, IndiaInstitute of Pharmaceutical Research, GLA University, Mathura 281406, IndiaDivision of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, JapanDrug Testing Laboratory, Avam Anusandhan Kendra, Raipur 490008, IndiaSchool of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, IndiaDepartment of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki 8892192, JapanSchool of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, IndiaSchool of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, IndiaDivision of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, JapanDivision of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, JapanCurrently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface mechanism. In this study, c-phycocyanin primed silver nano hybrids (AgcPCNP) were prepared and analyzed for properties such as cytotoxicity, metal decay, nanoconjugate stability, size expansion, and antioxidant features. Fluctuations in the expression of marker genes during cell migration phenomena in in vitro wound healing scenarios were also validated. Studies revealed that physiologically relevant ionic solutions did not exhibit any adverse effects on the nanoconjugate stability. However, acidic, alkali, and ethanol solutions completely denatured the AgcPCNP conjugates. Signal transduction RT<sup>2</sup>PCR array demonstrated that genes associated with NFĸB- and PI3K-pathways were significantly (<i>p</i> < 0.5%) altered between AgcPCNP and AgNP groups. Specific inhibitors of NFĸB (Nfi) and PI3K (LY294002) pathways confirmed the involvement of NFĸB signaling axes. In vitro wound healing assay demonstrated that NFĸB pathway plays a prime role in the fibroblast cell migration. In conclusion, the present investigation revealed that surface functionalized AgcPCNP accelerated the fibroblast cell migration and can be further explored for wound healing biomedical applications.https://www.mdpi.com/1422-0067/24/4/3184silver doped C-phycocyanin (AgcPCNP)cell migrationcell proliferationfibroblast cellsNFĸB pathwaywound healing |
| spellingShingle | Harishkumar Madhyastha Radha Madhyastha Eshika Chakraborty Kaushita Banerjee Kamal Shah Yuichi Nakajima Nagendra Singh Chauhan Sajitha Lulu Sudhakaran Kaoru Ohe Gothandam Kodiveri Muthukaliannan Abilash Valsala Gopalakrishnan Masugi Maruyama Nozomi Watanabe Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway International Journal of Molecular Sciences silver doped C-phycocyanin (AgcPCNP) cell migration cell proliferation fibroblast cells NFĸB pathway wound healing |
| title | Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway |
| title_full | Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway |
| title_fullStr | Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway |
| title_full_unstemmed | Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway |
| title_short | Fluro-Protein C-Phycocyanin Docked Silver Nanocomposite Accelerates Cell Migration through NFĸB Signaling Pathway |
| title_sort | fluro protein c phycocyanin docked silver nanocomposite accelerates cell migration through nfqb signaling pathway |
| topic | silver doped C-phycocyanin (AgcPCNP) cell migration cell proliferation fibroblast cells NFĸB pathway wound healing |
| url | https://www.mdpi.com/1422-0067/24/4/3184 |
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