Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols
ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in th...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057&lng=en&tlng=en |
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| author | SIMONE GOLUNSKI MARCELI F. SILVA CAMILA T. MARQUES VANUSA ROSSETO ROSILENE R. KAIZER ALTEMIR J. MOSSI DIANE RIGO ROGÉRIO M. DALLAGO MARCO DI LUCCIO HELEN TREICHEL |
| author_facet | SIMONE GOLUNSKI MARCELI F. SILVA CAMILA T. MARQUES VANUSA ROSSETO ROSILENE R. KAIZER ALTEMIR J. MOSSI DIANE RIGO ROGÉRIO M. DALLAGO MARCO DI LUCCIO HELEN TREICHEL |
| author_sort | SIMONE GOLUNSKI |
| collection | DOAJ |
| description | ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L-1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min. |
| first_indexed | 2024-12-12T00:45:14Z |
| format | Article |
| id | doaj.art-e887b9e146e54eefb122e75efbb3eca0 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-12T00:45:14Z |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-e887b9e146e54eefb122e75efbb3eca02022-12-22T00:44:09ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-2690891576310.1590/0001-3765201720160367S0001-37652017000100057Purification of inulinases by changing the ionic strength of the medium and precipitation with alcoholsSIMONE GOLUNSKIMARCELI F. SILVACAMILA T. MARQUESVANUSA ROSSETOROSILENE R. KAIZERALTEMIR J. MOSSIDIANE RIGOROGÉRIO M. DALLAGOMARCO DI LUCCIOHELEN TREICHELABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L-1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057&lng=en&tlng=eninulinasesionic strengthprecipitationlow cost. |
| spellingShingle | SIMONE GOLUNSKI MARCELI F. SILVA CAMILA T. MARQUES VANUSA ROSSETO ROSILENE R. KAIZER ALTEMIR J. MOSSI DIANE RIGO ROGÉRIO M. DALLAGO MARCO DI LUCCIO HELEN TREICHEL Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols Anais da Academia Brasileira de Ciências inulinases ionic strength precipitation low cost. |
| title | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| title_full | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| title_fullStr | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| title_full_unstemmed | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| title_short | Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| title_sort | purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols |
| topic | inulinases ionic strength precipitation low cost. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057&lng=en&tlng=en |
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