Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum
Deoxynivalenol(DON)，as a secondary metabolite of some fusarium species，has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis，the fermentation pH，temperature，supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON wer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of National Food and Strategic Reserves Administration
2020-07-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20200427?st=article_issue |
| Summary: | Deoxynivalenol(DON)，as a secondary metabolite of some fusarium species，has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis，the fermentation pH，temperature，supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON were studied in 5L-quadruple fermenters.The best fermentation conditions were determined，the fermentation pH was controlled at 7.5 and the temperature was controlled by stages，kept at 37 ℃ for first 12 hours，then raised to 39 ℃ until the end; 10 g/L molasses was added once at the 12th hour，then the fermentation was terminated at the 30th hour.Under the optimal conditions，the number of spores could reach 2.9×1010 CFU/mL and the spores’ rate could reach 96.7%.The fermentation liquid were further prepared into direct inoculated microbial inoculum and applied to remove DON from grain processing by-products; Under the condition that dry matter content in the composite material culture medium was not more than 30%，and the inoculation amount of the bacterium preparation was more than 0.1%，DON in the grain by-products could be effectively removed by fermentation and the highest degradation rate could reach 98.8%. |
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| ISSN: | 1007-7561 |