Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum
Deoxynivalenol(DON),as a secondary metabolite of some fusarium species,has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis,the fermentation pH,temperature,supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON wer...
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Academy of National Food and Strategic Reserves Administration
2020-07-01
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Series: | Liang you shipin ke-ji |
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Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20200427?st=article_issue |
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author | DU Wen CHANG Xiao-jiao ZHAO Yi-fan GUAN Yu-long SUN Chang-po LIU Hu-jun |
author_facet | DU Wen CHANG Xiao-jiao ZHAO Yi-fan GUAN Yu-long SUN Chang-po LIU Hu-jun |
author_sort | DU Wen |
collection | DOAJ |
description | Deoxynivalenol(DON),as a secondary metabolite of some fusarium species,has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis,the fermentation pH,temperature,supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON were studied in 5L-quadruple fermenters.The best fermentation conditions were determined,the fermentation pH was controlled at 7.5 and the temperature was controlled by stages,kept at 37 ℃ for first 12 hours,then raised to 39 ℃ until the end; 10 g/L molasses was added once at the 12th hour,then the fermentation was terminated at the 30th hour.Under the optimal conditions,the number of spores could reach 2.9×1010 CFU/mL and the spores’ rate could reach 96.7%.The fermentation liquid were further prepared into direct inoculated microbial inoculum and applied to remove DON from grain processing by-products; Under the condition that dry matter content in the composite material culture medium was not more than 30%,and the inoculation amount of the bacterium preparation was more than 0.1%,DON in the grain by-products could be effectively removed by fermentation and the highest degradation rate could reach 98.8%. |
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id | doaj.art-e88824773dcb41db9b9e29f8d10f4a5e |
institution | Directory Open Access Journal |
issn | 1007-7561 |
language | English |
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publishDate | 2020-07-01 |
publisher | Academy of National Food and Strategic Reserves Administration |
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series | Liang you shipin ke-ji |
spelling | doaj.art-e88824773dcb41db9b9e29f8d10f4a5e2023-06-13T06:40:39ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612020-07-0128415215810.16210/j.cnki.1007-7561.2020.04.024Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculumDU Wen0CHANG Xiao-jiao1ZHAO Yi-fan2GUAN Yu-long3SUN Chang-po4LIU Hu-jun5Academy of National Food and Strategic Reserves Administration,Beijing 100037,ChinaAcademy of National Food and Strategic Reserves Administration,Beijing 100037,ChinaAcademy of National Food and Strategic Reserves Administration,Beijing 100037,ChinaAnhui Gujing gongjiu Co.,Ltd.,Bozhou,Anhui 236800,ChinaAcademy of National Food and Strategic Reserves Administration,Beijing 100037,ChinaAcademy of National Food and Strategic Reserves Administration,Beijing 100037,ChinaDeoxynivalenol(DON),as a secondary metabolite of some fusarium species,has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis,the fermentation pH,temperature,supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON were studied in 5L-quadruple fermenters.The best fermentation conditions were determined,the fermentation pH was controlled at 7.5 and the temperature was controlled by stages,kept at 37 ℃ for first 12 hours,then raised to 39 ℃ until the end; 10 g/L molasses was added once at the 12th hour,then the fermentation was terminated at the 30th hour.Under the optimal conditions,the number of spores could reach 2.9×1010 CFU/mL and the spores’ rate could reach 96.7%.The fermentation liquid were further prepared into direct inoculated microbial inoculum and applied to remove DON from grain processing by-products; Under the condition that dry matter content in the composite material culture medium was not more than 30%,and the inoculation amount of the bacterium preparation was more than 0.1%,DON in the grain by-products could be effectively removed by fermentation and the highest degradation rate could reach 98.8%.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20200427?st=article_issuedeoxynivalenolbacillus subtilisoptimization of fermentation conditionsspores ratedon detoxification of grain by-products |
spellingShingle | DU Wen CHANG Xiao-jiao ZHAO Yi-fan GUAN Yu-long SUN Chang-po LIU Hu-jun Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum Liang you shipin ke-ji deoxynivalenol bacillus subtilis optimization of fermentation conditions spores rate don detoxification of grain by-products |
title | Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum |
title_full | Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum |
title_fullStr | Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum |
title_full_unstemmed | Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum |
title_short | Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum |
title_sort | research of fermentation preparation technology and preliminary application for deoxynivalenol degrading direct inoculated microbial inoculum |
topic | deoxynivalenol bacillus subtilis optimization of fermentation conditions spores rate don detoxification of grain by-products |
url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20200427?st=article_issue |
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