Molecular Methods for Identification and Quantification of Foodborne Pathogens
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne illness. We summarize simple, rapid, specific, and h...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/23/8262 |
| _version_ | 1827642957808795648 |
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| author | Min Zhang Jiajia Wu Zhaoai Shi Aocheng Cao Wensheng Fang Dongdong Yan Qiuxia Wang Yuan Li |
| author_facet | Min Zhang Jiajia Wu Zhaoai Shi Aocheng Cao Wensheng Fang Dongdong Yan Qiuxia Wang Yuan Li |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne illness. We summarize simple, rapid, specific, and highly effective molecular technology that is used to detect and identify foodborne pathogens, including polymerase chain reaction, isothermal amplification, loop-mediated isothermal amplification, nucleic acid sequence-based amplification, as well as gene chip and gene probe technology. The principles of their operation, the research supporting their application, and the advantages and disadvantages of each technology are summarized. |
| first_indexed | 2024-03-09T17:40:08Z |
| format | Article |
| id | doaj.art-e88b601032704236ba68b5245161af4b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:40:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e88b601032704236ba68b5245161af4b2023-11-24T11:39:19ZengMDPI AGMolecules1420-30492022-11-012723826210.3390/molecules27238262Molecular Methods for Identification and Quantification of Foodborne PathogensMin Zhang0Jiajia Wu1Zhaoai Shi2Aocheng Cao3Wensheng Fang4Dongdong Yan5Qiuxia Wang6Yuan Li7Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaFoodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne illness. We summarize simple, rapid, specific, and highly effective molecular technology that is used to detect and identify foodborne pathogens, including polymerase chain reaction, isothermal amplification, loop-mediated isothermal amplification, nucleic acid sequence-based amplification, as well as gene chip and gene probe technology. The principles of their operation, the research supporting their application, and the advantages and disadvantages of each technology are summarized.https://www.mdpi.com/1420-3049/27/23/8262polymerase chain reactionisothermal amplificationloop-mediated isothermal amplificationnucleic acid sequence-based amplificationgene chipgene probe technology |
| spellingShingle | Min Zhang Jiajia Wu Zhaoai Shi Aocheng Cao Wensheng Fang Dongdong Yan Qiuxia Wang Yuan Li Molecular Methods for Identification and Quantification of Foodborne Pathogens Molecules polymerase chain reaction isothermal amplification loop-mediated isothermal amplification nucleic acid sequence-based amplification gene chip gene probe technology |
| title | Molecular Methods for Identification and Quantification of Foodborne Pathogens |
| title_full | Molecular Methods for Identification and Quantification of Foodborne Pathogens |
| title_fullStr | Molecular Methods for Identification and Quantification of Foodborne Pathogens |
| title_full_unstemmed | Molecular Methods for Identification and Quantification of Foodborne Pathogens |
| title_short | Molecular Methods for Identification and Quantification of Foodborne Pathogens |
| title_sort | molecular methods for identification and quantification of foodborne pathogens |
| topic | polymerase chain reaction isothermal amplification loop-mediated isothermal amplification nucleic acid sequence-based amplification gene chip gene probe technology |
| url | https://www.mdpi.com/1420-3049/27/23/8262 |
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