Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation
In vascular plants, R2R3-MYB transcription factors are important regulators of secondary cell wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar genome, only a few members were characterized to participate in the regulation of the secondary cell wall biosynthesis. In...
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01262/full |
| _version_ | 1818838549063008256 |
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| author | Chaofeng Li Chaofeng Li Xiaodong Ma Xiaodong Ma Xiaodong Ma Hong Yu Yongyao Fu Keming Luo |
| author_facet | Chaofeng Li Chaofeng Li Xiaodong Ma Xiaodong Ma Xiaodong Ma Hong Yu Yongyao Fu Keming Luo |
| author_sort | Chaofeng Li |
| collection | DOAJ |
| description | In vascular plants, R2R3-MYB transcription factors are important regulators of secondary cell wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar genome, only a few members were characterized to participate in the regulation of the secondary cell wall biosynthesis. In this paper, we identify an R2R3-MYB transcription factor, PtoMYB74, which is predicted to be an ortholog of Arabidopsis AtMYB61, a transcription activator that regulates the secondary cell wall formation, lignin biosynthesis, stomatal aperture, and the mucilage of seed coat. PtoMYB74 is mainly expressed in the stems, especially in the xylem tissues and organs. PtoMYB74 as a transcriptional activator is localized to the nucleus. The overexpression of PtoMYB74 increased the secondary cell wall thickness of vessels in transgenic poplar and changed the secondary cell wall compositions. The expression levels of lignin and cellulose biosynthetic genes were elevated in the transgenic poplar overexpressing PtoMYB74 compared to the wild type, while there was no change in the xylan biosynthetic genes. Transcriptional activation assays demonstrated that PtoMYB74 could activate the promoters of structural genes in the lignin and cellulose biosynthetic pathways. Taken together, our data show that PtoMYB74 positively regulates the secondary cell wall biosynthesis in poplar. |
| first_indexed | 2024-12-19T03:40:09Z |
| format | Article |
| id | doaj.art-e88e2c7468654904b0bf97c7d64a737b |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T03:40:09Z |
| publishDate | 2018-10-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e88e2c7468654904b0bf97c7d64a737b2022-12-21T20:37:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-10-01910.3389/fpls.2018.01262360431Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall FormationChaofeng Li0Chaofeng Li1Xiaodong Ma2Xiaodong Ma3Xiaodong Ma4Hong Yu5Yongyao Fu6Keming Luo7Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaSchool of Ecology and Environmental Resources, Qinghai University for Nationalities, Xining, ChinaSchool of Life Sciences, Southwest University, Chongqing, ChinaSchool of Life Sciences, Southwest University, Chongqing, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaIn vascular plants, R2R3-MYB transcription factors are important regulators of secondary cell wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar genome, only a few members were characterized to participate in the regulation of the secondary cell wall biosynthesis. In this paper, we identify an R2R3-MYB transcription factor, PtoMYB74, which is predicted to be an ortholog of Arabidopsis AtMYB61, a transcription activator that regulates the secondary cell wall formation, lignin biosynthesis, stomatal aperture, and the mucilage of seed coat. PtoMYB74 is mainly expressed in the stems, especially in the xylem tissues and organs. PtoMYB74 as a transcriptional activator is localized to the nucleus. The overexpression of PtoMYB74 increased the secondary cell wall thickness of vessels in transgenic poplar and changed the secondary cell wall compositions. The expression levels of lignin and cellulose biosynthetic genes were elevated in the transgenic poplar overexpressing PtoMYB74 compared to the wild type, while there was no change in the xylan biosynthetic genes. Transcriptional activation assays demonstrated that PtoMYB74 could activate the promoters of structural genes in the lignin and cellulose biosynthetic pathways. Taken together, our data show that PtoMYB74 positively regulates the secondary cell wall biosynthesis in poplar.https://www.frontiersin.org/article/10.3389/fpls.2018.01262/fullPopulusR2R3-MYBtranscription factorssecondary cell walltransgenicgenetic |
| spellingShingle | Chaofeng Li Chaofeng Li Xiaodong Ma Xiaodong Ma Xiaodong Ma Hong Yu Yongyao Fu Keming Luo Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation Frontiers in Plant Science Populus R2R3-MYB transcription factors secondary cell wall transgenic genetic |
| title | Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation |
| title_full | Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation |
| title_fullStr | Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation |
| title_full_unstemmed | Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation |
| title_short | Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation |
| title_sort | ectopic expression of ptomyb74 in poplar and arabidopsis promotes secondary cell wall formation |
| topic | Populus R2R3-MYB transcription factors secondary cell wall transgenic genetic |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01262/full |
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