Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran
Background Mycobacterium bovis is the main cause of tuberculosis in cattle. The most commonly used method to identify bovis-infected cattle is tuberculin test. Objective The present study aimed to investigate the population structure of Mycobacterium bovis in infected cattle farms of Shiraz City i...
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Qazvin University of Medical Sciences & Health Services
2020-02-01
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| Series: | The Journal of Qazvin University of Medical Sciences |
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| Online Access: | http://journal.qums.ac.ir/article-1-2888-en.html |
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| author | Hossein Ghaderi Masoud Haghkhah Nader Mosavari Keyvan Tadayon |
| author_facet | Hossein Ghaderi Masoud Haghkhah Nader Mosavari Keyvan Tadayon |
| author_sort | Hossein Ghaderi |
| collection | DOAJ |
| description | Background Mycobacterium bovis is the main cause of tuberculosis in cattle. The most commonly used method to identify bovis-infected cattle is tuberculin test.
Objective The present study aimed to investigate the population structure of Mycobacterium bovis in infected cattle farms of Shiraz City in Iran.
Methods In this descriptive cross-sectional study, 50 pathological samples from tuberculin-positive cattle that were collected from two abattoirs were cultured on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in polymerase chain reaction (PCR)-16S rRNA, PCR-IS6110, and PCR--regions of difference (RD) typing. All the M. bovis isolates were then digested by PvuII restriction enzyme and genotyped by polymorphic guanine/cytosine-rich repetitive sequences (PGRS)-restriction fragment length polymorphism (RFLP) technique.
Findings In bacterial culture, 13 (26%) of samples had living mycobacteria where PCR test results revealed their identity as Mycobacterium bovis. Genotype profiling by RFLP-PGRS method displayed two patterns with 10 isolates shared a single profile identical to that of M. bovis bacillus calmette-guerin (BCG) strain (1173 P2) and three isolated with a different genotype.
Conclusion Higher prevalence of BCG-like M. bovis (as a typical characteristic of Iranian M. bovis population) in cattle farms of Shiraz City was expected. This may indicate the local evolution of new M. bovis strains in the region or the infiltration of such strains through cattle farming activities. |
| first_indexed | 2024-12-20T23:44:35Z |
| format | Article |
| id | doaj.art-e88e704cd9484b7984e5c38433f9dfcd |
| institution | Directory Open Access Journal |
| issn | 1561-3666 2228-7213 |
| language | fas |
| last_indexed | 2024-12-20T23:44:35Z |
| publishDate | 2020-02-01 |
| publisher | Qazvin University of Medical Sciences & Health Services |
| record_format | Article |
| series | The Journal of Qazvin University of Medical Sciences |
| spelling | doaj.art-e88e704cd9484b7984e5c38433f9dfcd2022-12-21T19:22:59ZfasQazvin University of Medical Sciences & Health ServicesThe Journal of Qazvin University of Medical Sciences1561-36662228-72132020-02-0123652653910.32598/JQUMS.23.6.5Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, IranHossein Ghaderihttps://orcid.org/0000-0002-6170-8710Masoud Haghkhahhttps://orcid.org/0000-0001-7266-4082Nader Mosavarihttps://orcid.org/0000-0002-3480-3376Keyvan Tadayonhttps://orcid.org/0000-0002-6927-2077Background Mycobacterium bovis is the main cause of tuberculosis in cattle. The most commonly used method to identify bovis-infected cattle is tuberculin test. Objective The present study aimed to investigate the population structure of Mycobacterium bovis in infected cattle farms of Shiraz City in Iran. Methods In this descriptive cross-sectional study, 50 pathological samples from tuberculin-positive cattle that were collected from two abattoirs were cultured on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in polymerase chain reaction (PCR)-16S rRNA, PCR-IS6110, and PCR--regions of difference (RD) typing. All the M. bovis isolates were then digested by PvuII restriction enzyme and genotyped by polymorphic guanine/cytosine-rich repetitive sequences (PGRS)-restriction fragment length polymorphism (RFLP) technique. Findings In bacterial culture, 13 (26%) of samples had living mycobacteria where PCR test results revealed their identity as Mycobacterium bovis. Genotype profiling by RFLP-PGRS method displayed two patterns with 10 isolates shared a single profile identical to that of M. bovis bacillus calmette-guerin (BCG) strain (1173 P2) and three isolated with a different genotype. Conclusion Higher prevalence of BCG-like M. bovis (as a typical characteristic of Iranian M. bovis population) in cattle farms of Shiraz City was expected. This may indicate the local evolution of new M. bovis strains in the region or the infiltration of such strains through cattle farming activities.http://journal.qums.ac.ir/article-1-2888-en.htmlmycobacterium bovistuberculinationpgrs-rflppvuiistrain |
| spellingShingle | Hossein Ghaderi Masoud Haghkhah Nader Mosavari Keyvan Tadayon Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran The Journal of Qazvin University of Medical Sciences mycobacterium bovis tuberculination pgrs-rflp pvuii strain |
| title | Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran |
| title_full | Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran |
| title_fullStr | Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran |
| title_full_unstemmed | Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran |
| title_short | Isolation, Molecular Identification and Genomic Pattern of Mycobacterium Bovis Isolates Collected from Tuberculin-positive Cattle in Infected Farms of Shiraz, Iran |
| title_sort | isolation molecular identification and genomic pattern of mycobacterium bovis isolates collected from tuberculin positive cattle in infected farms of shiraz iran |
| topic | mycobacterium bovis tuberculination pgrs-rflp pvuii strain |
| url | http://journal.qums.ac.ir/article-1-2888-en.html |
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