Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice
Atherosclerosis is the preliminary cause of coronary artery disease, one of the diseases that account for the largest number of fatal mortalities. Physical activity is an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated that changes in the proteomic profile are h...
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MDPI AG
2022-02-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/2/253 |
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| author | Chen-Chung Liao Jin-Wei Xu Wen-Ching Huang Hung-Chang Chang Yu-Tang Tung |
| author_facet | Chen-Chung Liao Jin-Wei Xu Wen-Ching Huang Hung-Chang Chang Yu-Tang Tung |
| author_sort | Chen-Chung Liao |
| collection | DOAJ |
| description | Atherosclerosis is the preliminary cause of coronary artery disease, one of the diseases that account for the largest number of fatal mortalities. Physical activity is an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated that changes in the proteomic profile are highly associated with atherosclerosis development, but the mechanism behind exercise for atherosclerosis amelioration has not yet been investigated from a proteomics perspective. Hence, the proteomic profiles could further elucidate the systematic effects of exercise intervention on ApoE knockout atherosclerotic model and high-fat-diet intervention. In the current study, Apoe<sup>em1Narl</sup>/Narl mice were randomly allocated into a normal diet (ND), Western diet (WD), and WD with 12-week exercise intervention (WD EX) groups. The plasma proteome between WD and WD EX groups demonstrate the significant difference, and ten major pathways, including cardiovascular disease (CVD)–hematological disease, inflammatory disease, infectious diseases, inflammatory response, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, metabolic disease_organismal injury and abnormalities, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, and endocrine system disorders_gastrointestinal disease, etc., were generated by the IPA analysis. The 15 proteins (MYOCD, PROS1, C2, SERPINA10, CRP, F5, C5, CFB, FGG, CFH, F12, PRDX2, PROZ, PPIA, and HABP2) critically involved in CVD–hematological disease pathway showed significant difference between WD and WD EX groups. In current study, exercise could significantly alleviate the significantly elevated C5 and inflammation induced by the WD group in accordance with amelioration of atherosclerosis. Therefore, exercise could mitigate chemotaxis through the modulation of the C5 level and innate immunity, thereby alleviating the pathogenesis of atherosclerosis in Western-diet-induced obese mice. |
| first_indexed | 2024-03-09T22:35:17Z |
| format | Article |
| id | doaj.art-e891d736403c483a995fc3ecbad846d1 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T22:35:17Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-e891d736403c483a995fc3ecbad846d12023-11-23T18:50:24ZengMDPI AGBiology2079-77372022-02-0111225310.3390/biology11020253Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout MiceChen-Chung Liao0Jin-Wei Xu1Wen-Ching Huang2Hung-Chang Chang3Yu-Tang Tung4Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Forestry, National Chung Hsing University, Taichung 402, TaiwanDepartment of Exercise and Health Science, National Taipei University of Nursing and Health Sciences, Taipei 112, TaiwanGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, TaiwanAtherosclerosis is the preliminary cause of coronary artery disease, one of the diseases that account for the largest number of fatal mortalities. Physical activity is an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated that changes in the proteomic profile are highly associated with atherosclerosis development, but the mechanism behind exercise for atherosclerosis amelioration has not yet been investigated from a proteomics perspective. Hence, the proteomic profiles could further elucidate the systematic effects of exercise intervention on ApoE knockout atherosclerotic model and high-fat-diet intervention. In the current study, Apoe<sup>em1Narl</sup>/Narl mice were randomly allocated into a normal diet (ND), Western diet (WD), and WD with 12-week exercise intervention (WD EX) groups. The plasma proteome between WD and WD EX groups demonstrate the significant difference, and ten major pathways, including cardiovascular disease (CVD)–hematological disease, inflammatory disease, infectious diseases, inflammatory response, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, metabolic disease_organismal injury and abnormalities, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, and endocrine system disorders_gastrointestinal disease, etc., were generated by the IPA analysis. The 15 proteins (MYOCD, PROS1, C2, SERPINA10, CRP, F5, C5, CFB, FGG, CFH, F12, PRDX2, PROZ, PPIA, and HABP2) critically involved in CVD–hematological disease pathway showed significant difference between WD and WD EX groups. In current study, exercise could significantly alleviate the significantly elevated C5 and inflammation induced by the WD group in accordance with amelioration of atherosclerosis. Therefore, exercise could mitigate chemotaxis through the modulation of the C5 level and innate immunity, thereby alleviating the pathogenesis of atherosclerosis in Western-diet-induced obese mice.https://www.mdpi.com/2079-7737/11/2/253atherosclerosiscomplement factor C5exerciseproteomic changeschemotaxis |
| spellingShingle | Chen-Chung Liao Jin-Wei Xu Wen-Ching Huang Hung-Chang Chang Yu-Tang Tung Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice Biology atherosclerosis complement factor C5 exercise proteomic changes chemotaxis |
| title | Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice |
| title_full | Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice |
| title_fullStr | Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice |
| title_full_unstemmed | Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice |
| title_short | Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice |
| title_sort | plasma proteomic changes of atherosclerosis after exercise in apoe knockout mice |
| topic | atherosclerosis complement factor C5 exercise proteomic changes chemotaxis |
| url | https://www.mdpi.com/2079-7737/11/2/253 |
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