Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus
Abstract Background Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in al...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2246-1 |
| _version_ | 1818507917830127616 |
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| author | Parvaneh Mehrbod Muna Ali Abdalla Fatemeh Fotouhi Masoumeh Heidarzadeh Abimbola O. Aro Jacobus N. Eloff Lyndy J. McGaw Folorunso O. Fasina |
| author_facet | Parvaneh Mehrbod Muna Ali Abdalla Fatemeh Fotouhi Masoumeh Heidarzadeh Abimbola O. Aro Jacobus N. Eloff Lyndy J. McGaw Folorunso O. Fasina |
| author_sort | Parvaneh Mehrbod |
| collection | DOAJ |
| description | Abstract Background Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in alternative use of herbal medications for prophylaxis against this infection. The South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez of the family Myrsinaceae was selected owing to its traditional use for the treatment of several diseases such as respiratory ailments and also previous preliminary studies of anti-influenza activity of its methanolic extract. The aim of this study was to investigate the immunomodulatory properties of a glycoside flavone isolated from RM against influenza A virus. Methods The non-cytotoxic concentration of the quercetin-3-O-α-L-rhamnopyranoside (Q3R) was determined by MTT assay and tested for activity against influenza A virus (IAV) in simultaneous, pre-penetration and post-penetration combination treatments over 1 h incubation on MDCK cells. The virus titer and viral load targeting NP and M2 viral genes were determined using HA and qPCR, respectively. TNF-α and IL-27 as pro- and anti-inflammatory cytokines were measured at RNA and protein levels by qPCR and ELISA, respectively. Results Quercetin-3-O-α-L-rhamnopyranoside at 150 μg/ml decreased the viral titer by 6 logs (p < 0.01) in the simultaneous procedure. The NP and M2 genes copy numbers as viral target genes, calculated based on the Ct values and standard formula, significantly decreased in simultaneous treatment (p < 0.01). The expression of cytokines was also considerably affected by the compound treatment. Conclusions This is the first report of quercetin-3-O-α-L-rhamnopyranoside from RM and its immunomodulatory properties against influenza A virus. Further research will focus on detecting the specific mechanism of virus-host interactions. |
| first_indexed | 2024-12-10T22:24:43Z |
| format | Article |
| id | doaj.art-e8920f96c56b4794bc8ceb2e37471153 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T22:24:43Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-e8920f96c56b4794bc8ceb2e374711532022-12-22T01:31:13ZengBMCBMC Complementary and Alternative Medicine1472-68822018-06-0118111010.1186/s12906-018-2246-1Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virusParvaneh Mehrbod0Muna Ali Abdalla1Fatemeh Fotouhi2Masoumeh Heidarzadeh3Abimbola O. Aro4Jacobus N. Eloff5Lyndy J. McGaw6Folorunso O. Fasina7Department of Veterinary Tropical Diseases, University of PretoriaPhytomedicine Programme, Department of Paraclinical Sciences, University of PretoriaInfluenza and Other Respiratory Viruses Department, Pasteur Institute of IRANInfluenza and Other Respiratory Viruses Department, Pasteur Institute of IRANPhytomedicine Programme, Department of Paraclinical Sciences, University of PretoriaPhytomedicine Programme, Department of Paraclinical Sciences, University of PretoriaPhytomedicine Programme, Department of Paraclinical Sciences, University of PretoriaDepartment of Veterinary Tropical Diseases, University of PretoriaAbstract Background Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in alternative use of herbal medications for prophylaxis against this infection. The South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez of the family Myrsinaceae was selected owing to its traditional use for the treatment of several diseases such as respiratory ailments and also previous preliminary studies of anti-influenza activity of its methanolic extract. The aim of this study was to investigate the immunomodulatory properties of a glycoside flavone isolated from RM against influenza A virus. Methods The non-cytotoxic concentration of the quercetin-3-O-α-L-rhamnopyranoside (Q3R) was determined by MTT assay and tested for activity against influenza A virus (IAV) in simultaneous, pre-penetration and post-penetration combination treatments over 1 h incubation on MDCK cells. The virus titer and viral load targeting NP and M2 viral genes were determined using HA and qPCR, respectively. TNF-α and IL-27 as pro- and anti-inflammatory cytokines were measured at RNA and protein levels by qPCR and ELISA, respectively. Results Quercetin-3-O-α-L-rhamnopyranoside at 150 μg/ml decreased the viral titer by 6 logs (p < 0.01) in the simultaneous procedure. The NP and M2 genes copy numbers as viral target genes, calculated based on the Ct values and standard formula, significantly decreased in simultaneous treatment (p < 0.01). The expression of cytokines was also considerably affected by the compound treatment. Conclusions This is the first report of quercetin-3-O-α-L-rhamnopyranoside from RM and its immunomodulatory properties against influenza A virus. Further research will focus on detecting the specific mechanism of virus-host interactions.http://link.springer.com/article/10.1186/s12906-018-2246-1Rapanea melanophloeosInfluenza a virusQuercetin-3-O-α-L-rhamnopyranosideCytokine |
| spellingShingle | Parvaneh Mehrbod Muna Ali Abdalla Fatemeh Fotouhi Masoumeh Heidarzadeh Abimbola O. Aro Jacobus N. Eloff Lyndy J. McGaw Folorunso O. Fasina Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus BMC Complementary and Alternative Medicine Rapanea melanophloeos Influenza a virus Quercetin-3-O-α-L-rhamnopyranoside Cytokine |
| title | Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus |
| title_full | Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus |
| title_fullStr | Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus |
| title_full_unstemmed | Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus |
| title_short | Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus |
| title_sort | immunomodulatory properties of quercetin 3 o α l rhamnopyranoside from rapanea melanophloeos against influenza a virus |
| topic | Rapanea melanophloeos Influenza a virus Quercetin-3-O-α-L-rhamnopyranoside Cytokine |
| url | http://link.springer.com/article/10.1186/s12906-018-2246-1 |
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