The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae
The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies. Here, we show that deletion of edc3, when combined with a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2016-10-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/5/10/1388 |
| _version_ | 1818461187974627328 |
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| author | Susanne Huch Maren Müller Mridula Muppavarapu Jessie Gommlich Vidya Balagopal Tracy Nissan |
| author_facet | Susanne Huch Maren Müller Mridula Muppavarapu Jessie Gommlich Vidya Balagopal Tracy Nissan |
| author_sort | Susanne Huch |
| collection | DOAJ |
| description | The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies. Here, we show that deletion of edc3, when combined with a removal of the glutamine/asparagine rich region of Lsm4 (edc3Δ lsm4ΔC) reduces mRNA stability and alters pathways of mRNA degradation. Multiple tested mRNAs exhibited reduced stability in the edc3Δ lsm4ΔC mutant. The destabilization was linked to an increased dependence on Ccr4-mediated deadenylation and mRNA decapping. Unlike characterized mutations in decapping factors that either are neutral or are able to stabilize mRNA, the combined edc3Δ lsm4ΔC mutant reduced mRNA stability. We characterized the growth and activity of the major mRNA decay systems and translation in double mutant and wild-type yeast. In the edc3Δ lsm4ΔC mutant, we observed alterations in the levels of specific mRNA decay factors as well as nuclear accumulation of the catalytic subunit of the decapping enzyme Dcp2. Hence, we suggest that the effects on mRNA stability in the edc3Δ lsm4ΔC mutant may originate from mRNA decay protein abundance or changes in mRNPs, or alternatively may imply a role for P bodies in mRNA stabilization. |
| first_indexed | 2024-12-14T23:42:10Z |
| format | Article |
| id | doaj.art-e893b5d4206d4225ac6369ff008d1931 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-14T23:42:10Z |
| publishDate | 2016-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-e893b5d4206d4225ac6369ff008d19312022-12-21T22:43:29ZengThe Company of BiologistsBiology Open2046-63902016-10-015101388139910.1242/bio.020487020487The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiaeSusanne Huch0Maren Müller1Mridula Muppavarapu2Jessie Gommlich3Vidya Balagopal4Tracy Nissan5 Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden Department of Molecular Biology, Umeå University, Umeå SE-901 87, Sweden The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies. Here, we show that deletion of edc3, when combined with a removal of the glutamine/asparagine rich region of Lsm4 (edc3Δ lsm4ΔC) reduces mRNA stability and alters pathways of mRNA degradation. Multiple tested mRNAs exhibited reduced stability in the edc3Δ lsm4ΔC mutant. The destabilization was linked to an increased dependence on Ccr4-mediated deadenylation and mRNA decapping. Unlike characterized mutations in decapping factors that either are neutral or are able to stabilize mRNA, the combined edc3Δ lsm4ΔC mutant reduced mRNA stability. We characterized the growth and activity of the major mRNA decay systems and translation in double mutant and wild-type yeast. In the edc3Δ lsm4ΔC mutant, we observed alterations in the levels of specific mRNA decay factors as well as nuclear accumulation of the catalytic subunit of the decapping enzyme Dcp2. Hence, we suggest that the effects on mRNA stability in the edc3Δ lsm4ΔC mutant may originate from mRNA decay protein abundance or changes in mRNPs, or alternatively may imply a role for P bodies in mRNA stabilization.http://bio.biologists.org/content/5/10/1388P bodiesDeadenylationExosomemRNA decappingmRNA decaymRNA stability |
| spellingShingle | Susanne Huch Maren Müller Mridula Muppavarapu Jessie Gommlich Vidya Balagopal Tracy Nissan The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae Biology Open P bodies Deadenylation Exosome mRNA decapping mRNA decay mRNA stability |
| title | The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae |
| title_full | The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae |
| title_fullStr | The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae |
| title_full_unstemmed | The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae |
| title_short | The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae |
| title_sort | decapping activator edc3 and the q n rich domain of lsm4 function together to enhance mrna stability and alter mrna decay pathway dependence in saccharomyces cerevisiae |
| topic | P bodies Deadenylation Exosome mRNA decapping mRNA decay mRNA stability |
| url | http://bio.biologists.org/content/5/10/1388 |
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