Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study
GBR (Guided Bone Regeneration) procedure is challenged by the risk of membrane exposure to the oral cavity and contamination. The barrier quality of these membranes serve as a mechanical block from bacterial penetration into the GBR site. The purpose of this in vitro study was to evaluate the antiba...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/16/5705 |
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| author | Helena Zelikman Gil Slutzkey Ofir Rosner Shifra Levartovsky Shlomo Matalon Ilan Beitlitum |
| author_facet | Helena Zelikman Gil Slutzkey Ofir Rosner Shifra Levartovsky Shlomo Matalon Ilan Beitlitum |
| author_sort | Helena Zelikman |
| collection | DOAJ |
| description | GBR (Guided Bone Regeneration) procedure is challenged by the risk of membrane exposure to the oral cavity and contamination. The barrier quality of these membranes serve as a mechanical block from bacterial penetration into the GBR site. The purpose of this in vitro study was to evaluate the antibacterial effect of three commercial non-resorbable polytetrafluoroethylene membranes. (Two d-PTFE membranes and one double layer e-PTFE +d-PTFE membrane). A validated in vitro model with two bacterial species (<i>Streptococcus sanguinis</i> and <i>Fusobacterium nucleatum</i>) was used. Eight samples from membrane each were placed in a 96-well microtiter plate. The experimental and positive control groups were exposed to a bacterial suspension which involved one bacterial species in each plate. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 h in temperature controlled microplate spectrophotometer under anaerobic conditions. One- Sample Kolmogorov–Smirnov Normal test and the Kruskal–Wallis test was used for the statistical analysis. As shown by the bacterial growth curves obtained from the spectrophotometer readings, all three membranes resulted in bacterial growth. We have not found a statistical difference in <i>F. nucleatum</i> growth between different membrane samples and the positive control group. However, <i>S. sanguinis</i> growth was reduced significantly in the presence of two membranes (CYTOPLAST TXT-200 and NeoGen<sup>TM</sup>) when compared to the control (<i>p</i> < 0.01). The presence of Permamem<sup>®</sup> had no significant influence on <i>S. sanguinis</i> growth. Some types of commercial non-resorbable PTFE membranes may have an impact on the growth dynamics of specific bacterial species. |
| first_indexed | 2024-03-09T13:02:15Z |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T13:02:15Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-e89795a52daf457a8043894ca16d4bc52023-11-30T21:53:38ZengMDPI AGMaterials1996-19442022-08-011516570510.3390/ma15165705Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro StudyHelena Zelikman0Gil Slutzkey1Ofir Rosner2Shifra Levartovsky3Shlomo Matalon4Ilan Beitlitum5Department of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Periodontology and Dental Implantology, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Periodontology and Dental Implantology, Goldschleger School of Dental Medicine, Sackler Medical Faculty, Tel Aviv University, Tel Aviv 6997801, IsraelGBR (Guided Bone Regeneration) procedure is challenged by the risk of membrane exposure to the oral cavity and contamination. The barrier quality of these membranes serve as a mechanical block from bacterial penetration into the GBR site. The purpose of this in vitro study was to evaluate the antibacterial effect of three commercial non-resorbable polytetrafluoroethylene membranes. (Two d-PTFE membranes and one double layer e-PTFE +d-PTFE membrane). A validated in vitro model with two bacterial species (<i>Streptococcus sanguinis</i> and <i>Fusobacterium nucleatum</i>) was used. Eight samples from membrane each were placed in a 96-well microtiter plate. The experimental and positive control groups were exposed to a bacterial suspension which involved one bacterial species in each plate. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 h in temperature controlled microplate spectrophotometer under anaerobic conditions. One- Sample Kolmogorov–Smirnov Normal test and the Kruskal–Wallis test was used for the statistical analysis. As shown by the bacterial growth curves obtained from the spectrophotometer readings, all three membranes resulted in bacterial growth. We have not found a statistical difference in <i>F. nucleatum</i> growth between different membrane samples and the positive control group. However, <i>S. sanguinis</i> growth was reduced significantly in the presence of two membranes (CYTOPLAST TXT-200 and NeoGen<sup>TM</sup>) when compared to the control (<i>p</i> < 0.01). The presence of Permamem<sup>®</sup> had no significant influence on <i>S. sanguinis</i> growth. Some types of commercial non-resorbable PTFE membranes may have an impact on the growth dynamics of specific bacterial species.https://www.mdpi.com/1996-1944/15/16/5705barrier membranesGBRmembrane exposurebacterial contamination |
| spellingShingle | Helena Zelikman Gil Slutzkey Ofir Rosner Shifra Levartovsky Shlomo Matalon Ilan Beitlitum Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study Materials barrier membranes GBR membrane exposure bacterial contamination |
| title | Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study |
| title_full | Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study |
| title_fullStr | Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study |
| title_full_unstemmed | Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study |
| title_short | Bacterial Growth on Three Non-Resorbable Polytetrafluoroethylene (PTFE) Membranes—An In Vitro Study |
| title_sort | bacterial growth on three non resorbable polytetrafluoroethylene ptfe membranes an in vitro study |
| topic | barrier membranes GBR membrane exposure bacterial contamination |
| url | https://www.mdpi.com/1996-1944/15/16/5705 |
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