Roles of HOTAIR in lung cancer susceptibility and prognosis
Abstract Background Long noncoding (lncRNA) single‐nucleotide polymorphisms (SNPs) are associated with the susceptibility to the development of various malignant tumors. The aim of this study was to investigate the roles of HOX transcript antisense intergenic RNA (HOTAIR) and its SNPs in lung cancer...
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.1299 |
| _version_ | 1818392079406989312 |
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| author | Meng‐Meng Ren Sen Xu Yu‐Bo Wei Juan‐Juan Yang Ya‐Nan Yang Shan‐Shan Sun You‐Jie Li Ping‐Yu Wang Shu‐Yang Xie |
| author_facet | Meng‐Meng Ren Sen Xu Yu‐Bo Wei Juan‐Juan Yang Ya‐Nan Yang Shan‐Shan Sun You‐Jie Li Ping‐Yu Wang Shu‐Yang Xie |
| author_sort | Meng‐Meng Ren |
| collection | DOAJ |
| description | Abstract Background Long noncoding (lncRNA) single‐nucleotide polymorphisms (SNPs) are associated with the susceptibility to the development of various malignant tumors. The aim of this study was to investigate the roles of HOX transcript antisense intergenic RNA (HOTAIR) and its SNPs in lung cancer. Methods Initially, the expression of HOTAIR in different tumors was investigated using the online Gene Expression Profiling Interactive Analysis (GEPIA) resource. Three SNPs (rs920778, rs1899663, and rs4759314) of HOTAIR were identified using the MassArray system. Following this, the relationship between these SNPs and susceptibility to lung cancer was investigated. Results Expression of HOTAIR was found to increase in a variety of cancers, including nonsmall cell lung cancer (NSCLC). We found that the genotypes of these SNPs (rs920778, rs1899663, and rs4759314) were not significantly associated with lung cancer type, family history, lymph node metastasis, or lung cancer stage. In gender stratification, the results of rs920778 genotypes showed that, compared to genotype AA, the AG (OR = 0.344, 95% CI: 0.133–0.893, p = .028) and AG + GG (OR = 0.378, 95% CI: 0.153–0.932, p = .035) genotypes of rs920778 are protective factors against NSCLC in females. In smoking stratification, compared with AA of rs920778, the genotype AG + GG (OR = 0.507, 95% CI: 0.263–0.975, p = .042) was a protective factor against NSCLC in nonsmoking people. No statistical differences were observed in the classifications of rs1899663 and rs4759314 genotypes. Linkage disequilibrium analysis revealed a high linkage disequilibrium between the rs920778 and rs1899663 (D′ = 0.99, r2 = .74), rs920778 and rs4759314 (D′ = 0.85, r2 = .13), and rs1899663 and rs4759314 (D′ = 0.79, r2 = .00). Conclusion Our study demonstrated that HOTAIR expression increased in NSCLC, and that the genotypes of rs920778 could be useful in the diagnosis and prognosis of lung cancer. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
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| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-e8989e6b15a748b0b7fcd1d8be855c562022-12-21T23:15:35ZengWileyMolecular Genetics & Genomic Medicine2324-92692020-07-0187n/an/a10.1002/mgg3.1299Roles of HOTAIR in lung cancer susceptibility and prognosisMeng‐Meng Ren0Sen Xu1Yu‐Bo Wei2Juan‐Juan Yang3Ya‐Nan Yang4Shan‐Shan Sun5You‐Jie Li6Ping‐Yu Wang7Shu‐Yang Xie8Department of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDongying People’s HospitalBinzhou Medical College Affiliated Teaching Hospital Dongying P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDepartment of Epidemiology Binzhou Medical University YanTai P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaDepartment of Biochemistry and Molecular Biology Key Laboratory of Tumor Molecular Biology in Binzhou Medical UniversityBinzhou Medical University YanTai P.R. ChinaAbstract Background Long noncoding (lncRNA) single‐nucleotide polymorphisms (SNPs) are associated with the susceptibility to the development of various malignant tumors. The aim of this study was to investigate the roles of HOX transcript antisense intergenic RNA (HOTAIR) and its SNPs in lung cancer. Methods Initially, the expression of HOTAIR in different tumors was investigated using the online Gene Expression Profiling Interactive Analysis (GEPIA) resource. Three SNPs (rs920778, rs1899663, and rs4759314) of HOTAIR were identified using the MassArray system. Following this, the relationship between these SNPs and susceptibility to lung cancer was investigated. Results Expression of HOTAIR was found to increase in a variety of cancers, including nonsmall cell lung cancer (NSCLC). We found that the genotypes of these SNPs (rs920778, rs1899663, and rs4759314) were not significantly associated with lung cancer type, family history, lymph node metastasis, or lung cancer stage. In gender stratification, the results of rs920778 genotypes showed that, compared to genotype AA, the AG (OR = 0.344, 95% CI: 0.133–0.893, p = .028) and AG + GG (OR = 0.378, 95% CI: 0.153–0.932, p = .035) genotypes of rs920778 are protective factors against NSCLC in females. In smoking stratification, compared with AA of rs920778, the genotype AG + GG (OR = 0.507, 95% CI: 0.263–0.975, p = .042) was a protective factor against NSCLC in nonsmoking people. No statistical differences were observed in the classifications of rs1899663 and rs4759314 genotypes. Linkage disequilibrium analysis revealed a high linkage disequilibrium between the rs920778 and rs1899663 (D′ = 0.99, r2 = .74), rs920778 and rs4759314 (D′ = 0.85, r2 = .13), and rs1899663 and rs4759314 (D′ = 0.79, r2 = .00). Conclusion Our study demonstrated that HOTAIR expression increased in NSCLC, and that the genotypes of rs920778 could be useful in the diagnosis and prognosis of lung cancer.https://doi.org/10.1002/mgg3.1299HOTAIR genelung cancerprognosisSNPssusceptibility |
| spellingShingle | Meng‐Meng Ren Sen Xu Yu‐Bo Wei Juan‐Juan Yang Ya‐Nan Yang Shan‐Shan Sun You‐Jie Li Ping‐Yu Wang Shu‐Yang Xie Roles of HOTAIR in lung cancer susceptibility and prognosis Molecular Genetics & Genomic Medicine HOTAIR gene lung cancer prognosis SNPs susceptibility |
| title | Roles of HOTAIR in lung cancer susceptibility and prognosis |
| title_full | Roles of HOTAIR in lung cancer susceptibility and prognosis |
| title_fullStr | Roles of HOTAIR in lung cancer susceptibility and prognosis |
| title_full_unstemmed | Roles of HOTAIR in lung cancer susceptibility and prognosis |
| title_short | Roles of HOTAIR in lung cancer susceptibility and prognosis |
| title_sort | roles of hotair in lung cancer susceptibility and prognosis |
| topic | HOTAIR gene lung cancer prognosis SNPs susceptibility |
| url | https://doi.org/10.1002/mgg3.1299 |
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