Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method

Apo pickle is a traditional Chinese fermented vegetable. However, the traditional fermentation process of Apo pickle is slow, easy to ruin, and cannot be judged with regard to time. To improve fermentation, LP-165 (<i>L. Plantarum</i>), which has a high salt tolerance, acidification, and...

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Main Authors: Mengyao Wang, Jiawei Liu, Lu Huang, Haiying Liu
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/22/3577
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author Mengyao Wang
Jiawei Liu
Lu Huang
Haiying Liu
author_facet Mengyao Wang
Jiawei Liu
Lu Huang
Haiying Liu
author_sort Mengyao Wang
collection DOAJ
description Apo pickle is a traditional Chinese fermented vegetable. However, the traditional fermentation process of Apo pickle is slow, easy to ruin, and cannot be judged with regard to time. To improve fermentation, LP-165 (<i>L. Plantarum</i>), which has a high salt tolerance, acidification, and growth capacity, was chosen as the starter culture. Meanwhile, a colorimetric sensor array (CSA) sensitive to pickle volatile compounds was developed to differentiate Apo pickles at varying degrees of fermentation. The color components were extracted from each dye in the color change profiles and were analyzed using principal component analysis (PCA) and linear discriminant analysis (LDA). The fermentation process of the Apo pickle was classified into four phases by LDA. The accuracy of backward substitution verification was 99% and the accuracy of cross validation was 92.7%. Furthermore, the partial least squares regression (PLSR) showed that data from the CSA were correlated with pH total acid, lactic acid, and volatile acids of the Apo pickle. These results illustrate that the CSA reacts quickly to inoculated Apo pickle and could be used to detect fermentation.
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spelling doaj.art-e89c6d57895845a18bf12bd8e8ebeda02023-11-24T08:20:58ZengMDPI AGFoods2304-81582022-11-011122357710.3390/foods11223577Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array MethodMengyao Wang0Jiawei Liu1Lu Huang2Haiying Liu3State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214000, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214000, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214000, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214000, ChinaApo pickle is a traditional Chinese fermented vegetable. However, the traditional fermentation process of Apo pickle is slow, easy to ruin, and cannot be judged with regard to time. To improve fermentation, LP-165 (<i>L. Plantarum</i>), which has a high salt tolerance, acidification, and growth capacity, was chosen as the starter culture. Meanwhile, a colorimetric sensor array (CSA) sensitive to pickle volatile compounds was developed to differentiate Apo pickles at varying degrees of fermentation. The color components were extracted from each dye in the color change profiles and were analyzed using principal component analysis (PCA) and linear discriminant analysis (LDA). The fermentation process of the Apo pickle was classified into four phases by LDA. The accuracy of backward substitution verification was 99% and the accuracy of cross validation was 92.7%. Furthermore, the partial least squares regression (PLSR) showed that data from the CSA were correlated with pH total acid, lactic acid, and volatile acids of the Apo pickle. These results illustrate that the CSA reacts quickly to inoculated Apo pickle and could be used to detect fermentation.https://www.mdpi.com/2304-8158/11/22/3577Apo picklecolorimetric sensor arrayfermentationlinear discriminant analysispartial least squares regression
spellingShingle Mengyao Wang
Jiawei Liu
Lu Huang
Haiying Liu
Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
Foods
Apo pickle
colorimetric sensor array
fermentation
linear discriminant analysis
partial least squares regression
title Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
title_full Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
title_fullStr Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
title_full_unstemmed Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
title_short Detection of the Inoculated Fermentation Process of Apo Pickle Based on a Colorimetric Sensor Array Method
title_sort detection of the inoculated fermentation process of apo pickle based on a colorimetric sensor array method
topic Apo pickle
colorimetric sensor array
fermentation
linear discriminant analysis
partial least squares regression
url https://www.mdpi.com/2304-8158/11/22/3577
work_keys_str_mv AT mengyaowang detectionoftheinoculatedfermentationprocessofapopicklebasedonacolorimetricsensorarraymethod
AT jiaweiliu detectionoftheinoculatedfermentationprocessofapopicklebasedonacolorimetricsensorarraymethod
AT luhuang detectionoftheinoculatedfermentationprocessofapopicklebasedonacolorimetricsensorarraymethod
AT haiyingliu detectionoftheinoculatedfermentationprocessofapopicklebasedonacolorimetricsensorarraymethod