Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype
IntroductionTo establish a new model for exploring the mechanism of the gut microbiome and drug metabolism, we explored whether Taohong Siwu Decoction acts after metabolism by intestinal flora under the premise of clarifying the interaction between intestinal flora and drug metabolism.MethodsTaohong...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1192589/full |
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| author | Suyin Feng Suyin Feng Quan Wan Weijiang Wu Chenyang Zhang Chenyang Zhang Hua Lu Xiaojie Lu Xiaojie Lu Xiaojie Lu |
| author_facet | Suyin Feng Suyin Feng Quan Wan Weijiang Wu Chenyang Zhang Chenyang Zhang Hua Lu Xiaojie Lu Xiaojie Lu Xiaojie Lu |
| author_sort | Suyin Feng |
| collection | DOAJ |
| description | IntroductionTo establish a new model for exploring the mechanism of the gut microbiome and drug metabolism, we explored whether Taohong Siwu Decoction acts after metabolism by intestinal flora under the premise of clarifying the interaction between intestinal flora and drug metabolism.MethodsTaohong Siwu Decoction (TSD) was fed to germ-free mice and conventional mice, respectively. The serum from both groups of mice was removed and co-cultured with glioma cells in vitro. The co-cultured glioma cells were compared separately for changes at the RNA level using RNA-seq technology. The genes of interest in the comparison results were selected for validation.ResultsThe differences in the phenotypic alterations of glioma cells between serum from TSD-fed germ-free mice and normal mice were statistically significant. In vitro experiments showed that Taohong Siwu Decoction-fed normal mouse serum-stimulated glioma cells, which inhibited proliferation and increased autophagy. RNA-seq analysis showed that TSD-fed normal mouse serum could regulate CDC6 pathway activity in glioma cells. The therapeutic effect of TSD is significantly influenced by intestinal flora.ConclusionThe treatment of tumors by TSD may be modulated by intestinal flora. We established a new method to quantify the relationship between intestinal flora and the regulation of TSD efficacy through this study. |
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| format | Article |
| id | doaj.art-e89cbc052ee647888904865eb759ec89 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-13T07:17:34Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e89cbc052ee647888904865eb759ec892023-06-05T05:08:26ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-06-011310.3389/fcimb.2023.11925891192589Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotypeSuyin Feng0Suyin Feng1Quan Wan2Weijiang Wu3Chenyang Zhang4Chenyang Zhang5Hua Lu6Xiaojie Lu7Xiaojie Lu8Xiaojie Lu9Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaNeuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaJiangnan University Medical Center, Wuxi, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaNeuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaInstitute of Integrative Chinese and Western Medicine, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaNeuroscience Center, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaJiangnan University Medical Center, Wuxi, ChinaWuxi Neurological Institute, Wuxi No.2 People’s Hospital, Wuxi, ChinaIntroductionTo establish a new model for exploring the mechanism of the gut microbiome and drug metabolism, we explored whether Taohong Siwu Decoction acts after metabolism by intestinal flora under the premise of clarifying the interaction between intestinal flora and drug metabolism.MethodsTaohong Siwu Decoction (TSD) was fed to germ-free mice and conventional mice, respectively. The serum from both groups of mice was removed and co-cultured with glioma cells in vitro. The co-cultured glioma cells were compared separately for changes at the RNA level using RNA-seq technology. The genes of interest in the comparison results were selected for validation.ResultsThe differences in the phenotypic alterations of glioma cells between serum from TSD-fed germ-free mice and normal mice were statistically significant. In vitro experiments showed that Taohong Siwu Decoction-fed normal mouse serum-stimulated glioma cells, which inhibited proliferation and increased autophagy. RNA-seq analysis showed that TSD-fed normal mouse serum could regulate CDC6 pathway activity in glioma cells. The therapeutic effect of TSD is significantly influenced by intestinal flora.ConclusionThe treatment of tumors by TSD may be modulated by intestinal flora. We established a new method to quantify the relationship between intestinal flora and the regulation of TSD efficacy through this study.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1192589/fullTaohong Siwu Decoctionintestinal floraCDC6 pathwayglioma cellsRNA-seq analysis |
| spellingShingle | Suyin Feng Suyin Feng Quan Wan Weijiang Wu Chenyang Zhang Chenyang Zhang Hua Lu Xiaojie Lu Xiaojie Lu Xiaojie Lu Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype Frontiers in Cellular and Infection Microbiology Taohong Siwu Decoction intestinal flora CDC6 pathway glioma cells RNA-seq analysis |
| title | Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype |
| title_full | Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype |
| title_fullStr | Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype |
| title_full_unstemmed | Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype |
| title_short | Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype |
| title_sort | effect of gut microbiome regulated taohong siwu decoction metabolism on glioma cell phenotype |
| topic | Taohong Siwu Decoction intestinal flora CDC6 pathway glioma cells RNA-seq analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1192589/full |
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