Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats
The aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-12-01
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| Series: | Reviews in Analytical Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/revac-2020-0104 |
| _version_ | 1818666729419571200 |
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| author | Bobrowska-Korczak Barbara Domanska Kamila Skrajnowska Dorota Wrzesien Robert Giebultowicz Joanna Bielecki Wojciech Wyrebiak Rafał Piotrowska Urszula Sobczak Marcin Kałużna-Czaplińska Joanna |
| author_facet | Bobrowska-Korczak Barbara Domanska Kamila Skrajnowska Dorota Wrzesien Robert Giebultowicz Joanna Bielecki Wojciech Wyrebiak Rafał Piotrowska Urszula Sobczak Marcin Kałużna-Czaplińska Joanna |
| author_sort | Bobrowska-Korczak Barbara |
| collection | DOAJ |
| description | The aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley rats divided into 3 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano and micro-sized zinc particles. To induce the mammary cancer (adenocarcinoma), rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a validated high performance liquid chromatography coupled to mass spectrometry method. In the first stage of investigations a synergistic activity of nanosized Zn with DMBA on the growth of the neoplastic process was found. During that time a statistically significant increase in the levels of all six examined markers in the rats’ urine was observed. However, as the experiment continued, the supplementation with nanosized zinc caused inhibition of tumour growth, being followed by regression and remission of tumours, as well as, a statistically significant systematic reduction of the levels of methyl derivatives in the urine. Biopsy images indicated grade 1 tumours with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, moderately-differentiated grade 2 adenocarcinoma was identified. It was found that the biological activity of zinc depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression. |
| first_indexed | 2024-12-17T06:09:09Z |
| format | Article |
| id | doaj.art-e8a0adb451b84e138252706d1d392161 |
| institution | Directory Open Access Journal |
| issn | 0793-0135 2191-0189 |
| language | English |
| last_indexed | 2024-12-17T06:09:09Z |
| publishDate | 2020-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Reviews in Analytical Chemistry |
| spelling | doaj.art-e8a0adb451b84e138252706d1d3921612022-12-21T22:00:41ZengDe GruyterReviews in Analytical Chemistry0793-01352191-01892020-12-0139120020810.1515/revac-2020-0104revac-2020-0104Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in ratsBobrowska-Korczak Barbara0Domanska Kamila1Skrajnowska Dorota2Wrzesien Robert3Giebultowicz Joanna4Bielecki Wojciech5Wyrebiak Rafał6Piotrowska Urszula7Sobczak Marcin8Kałużna-Czaplińska Joanna9Department of Bromatology, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Bromatology, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Bromatology, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandCentral Laboratory of Experimental Animals, Medical University of Warsaw, Poland, Banacha 1a, 02-091Warsaw, PolandDepartment of Drug Analysis, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Live Sciences, Nowoursynowska 159c, 02-787Warsaw, PolandDepartment of Biomaterials Chemistry, Chair of Analytical Chemistry and Biomaterials, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Biomaterials Chemistry, Chair of Analytical Chemistry and Biomaterials, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Biomaterials Chemistry, Chair of Analytical Chemistry and Biomaterials, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Banacha 1, 02-097Warsaw, PolandDepartment of Chemistry, Institute of General and Ecological Chemistry, Lodz University of Technology, Lodz, PolandThe aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley rats divided into 3 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano and micro-sized zinc particles. To induce the mammary cancer (adenocarcinoma), rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a validated high performance liquid chromatography coupled to mass spectrometry method. In the first stage of investigations a synergistic activity of nanosized Zn with DMBA on the growth of the neoplastic process was found. During that time a statistically significant increase in the levels of all six examined markers in the rats’ urine was observed. However, as the experiment continued, the supplementation with nanosized zinc caused inhibition of tumour growth, being followed by regression and remission of tumours, as well as, a statistically significant systematic reduction of the levels of methyl derivatives in the urine. Biopsy images indicated grade 1 tumours with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, moderately-differentiated grade 2 adenocarcinoma was identified. It was found that the biological activity of zinc depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression.https://doi.org/10.1515/revac-2020-0104nanosized zincmicrosized zincepigeneticcancer |
| spellingShingle | Bobrowska-Korczak Barbara Domanska Kamila Skrajnowska Dorota Wrzesien Robert Giebultowicz Joanna Bielecki Wojciech Wyrebiak Rafał Piotrowska Urszula Sobczak Marcin Kałużna-Czaplińska Joanna Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats Reviews in Analytical Chemistry nanosized zinc microsized zinc epigenetic cancer |
| title | Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats |
| title_full | Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats |
| title_fullStr | Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats |
| title_full_unstemmed | Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats |
| title_short | Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats |
| title_sort | nanosized zinc epigenetic changes and its relationship with dmba induced breast cancer in rats |
| topic | nanosized zinc microsized zinc epigenetic cancer |
| url | https://doi.org/10.1515/revac-2020-0104 |
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