Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress
Oxidative stress is a main cause of tissue damage and highly associated with incidence of human chronic diseases. Among the major target organs attacked by reactive oxygen species (ROS) is the liver. Protocatechuic acid (PCA) is a phenolic compound found in green tea, acai oil and some mushroom spec...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122000065 |
| _version_ | 1811188697393004544 |
|---|---|
| author | Wu-Joo Lee Seong-Ho Lee |
| author_facet | Wu-Joo Lee Seong-Ho Lee |
| author_sort | Wu-Joo Lee |
| collection | DOAJ |
| description | Oxidative stress is a main cause of tissue damage and highly associated with incidence of human chronic diseases. Among the major target organs attacked by reactive oxygen species (ROS) is the liver. Protocatechuic acid (PCA) is a phenolic compound found in green tea, acai oil and some mushroom species that possesses strong antioxidative and anti-inflammatory activity and may have benefits as a natural phytochemical for prevention of human diseases. However, the protective effect of PCA on hydrogen peroxide (H2O2)-induced oxidative stress specifically in the liver has not yet been investigated. The current study aims to observe if PCA possesses protective activity against H2O2-induced oxidative stress in HepG2 human liver cancer cells. Relative to untreated control cells, treatment of HepG2 cells with PCA reduced H2O2-induced cell death and mitigated H2O2-induced production of ROS; furthermore, it mitigated the H2O2-induced increase of caspase-3/7 enzyme activity, expression of cleaved poly(ADP-ribose) polymerase (PARP), expression of endoplasmic reticulum (ER) stress genes including activating transcription factor 4 (ATF4), serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) and phosphorylation of p38 mitogen-activated protein kinases (MAPK). These findings indicate that PCA effectively protects hepatic cells from H2O2-induced oxidative stress and cell death. |
| first_indexed | 2024-04-11T14:22:46Z |
| format | Article |
| id | doaj.art-e8a2a13e1d7d4dd29ce4c30f2a397ac2 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-04-11T14:22:46Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-e8a2a13e1d7d4dd29ce4c30f2a397ac22022-12-22T04:19:00ZengElsevierCurrent Research in Food Science2665-92712022-01-015222227Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stressWu-Joo Lee0Seong-Ho Lee1Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, 20742, USACorresponding author. Department of Nutrition and Food Science, University of Maryland, College Park, MD, 20742, USA.; Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, 20742, USAOxidative stress is a main cause of tissue damage and highly associated with incidence of human chronic diseases. Among the major target organs attacked by reactive oxygen species (ROS) is the liver. Protocatechuic acid (PCA) is a phenolic compound found in green tea, acai oil and some mushroom species that possesses strong antioxidative and anti-inflammatory activity and may have benefits as a natural phytochemical for prevention of human diseases. However, the protective effect of PCA on hydrogen peroxide (H2O2)-induced oxidative stress specifically in the liver has not yet been investigated. The current study aims to observe if PCA possesses protective activity against H2O2-induced oxidative stress in HepG2 human liver cancer cells. Relative to untreated control cells, treatment of HepG2 cells with PCA reduced H2O2-induced cell death and mitigated H2O2-induced production of ROS; furthermore, it mitigated the H2O2-induced increase of caspase-3/7 enzyme activity, expression of cleaved poly(ADP-ribose) polymerase (PARP), expression of endoplasmic reticulum (ER) stress genes including activating transcription factor 4 (ATF4), serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) and phosphorylation of p38 mitogen-activated protein kinases (MAPK). These findings indicate that PCA effectively protects hepatic cells from H2O2-induced oxidative stress and cell death.http://www.sciencedirect.com/science/article/pii/S2665927122000065Protocatechuic acidHepatocytesHepG2Oxidative stressApoptosis |
| spellingShingle | Wu-Joo Lee Seong-Ho Lee Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress Current Research in Food Science Protocatechuic acid Hepatocytes HepG2 Oxidative stress Apoptosis |
| title | Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress |
| title_full | Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress |
| title_fullStr | Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress |
| title_full_unstemmed | Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress |
| title_short | Protocatechuic acid protects hepatocytes against hydrogen peroxide-induced oxidative stress |
| title_sort | protocatechuic acid protects hepatocytes against hydrogen peroxide induced oxidative stress |
| topic | Protocatechuic acid Hepatocytes HepG2 Oxidative stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2665927122000065 |
| work_keys_str_mv | AT wujoolee protocatechuicacidprotectshepatocytesagainsthydrogenperoxideinducedoxidativestress AT seongholee protocatechuicacidprotectshepatocytesagainsthydrogenperoxideinducedoxidativestress |