DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
Abstract Plant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status o...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-10237-x |
| _version_ | 1797220034996600832 |
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| author | Shuo Wang XueLei Zhao Chang Li Jing Dong JiaCheng Ma YueHong Long ZhaoBin Xing |
| author_facet | Shuo Wang XueLei Zhao Chang Li Jing Dong JiaCheng Ma YueHong Long ZhaoBin Xing |
| author_sort | Shuo Wang |
| collection | DOAJ |
| description | Abstract Plant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status of plants, and these modifications can directly impact gene expression and product synthesis by changing the DNA methylation status of functional genes involved in secondary metabolite synthesis. However, further research is needed to fully understand the extent to which DNA methylation modifies the content of secondary metabolites to mediate plants’ responses to drought stress, as well as the underlying mechanisms involved. Our study found that in Eleutherococcus senticosus (E. senticosus), moderate water deprivation significantly decreased DNA methylation levels throughout the genome and at the promoters of EsFPS, EsSS, and EsSE. Transcription factors like EsMYB-r1, previously inhibited by DNA methylation, can re-bind to the EsFPS promotor region following DNA demethylation. This process promotes gene expression and, ultimately, saponin synthesis and accumulation. The increased saponin levels in E. senticosus acted as antioxidants, enhancing the plant’s adaptability to drought stress. |
| first_indexed | 2024-04-24T12:43:08Z |
| format | Article |
| id | doaj.art-e8a511635ca94b29ac86e453abc72d50 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-24T12:43:08Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-e8a511635ca94b29ac86e453abc72d502024-04-07T11:09:25ZengBMCBMC Genomics1471-21642024-04-0125111810.1186/s12864-024-10237-xDNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stressShuo Wang0XueLei Zhao1Chang Li2Jing Dong3JiaCheng Ma4YueHong Long5ZhaoBin Xing6College of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyCollege of Life Sciences, North China University of Science and TechnologyAbstract Plant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status of plants, and these modifications can directly impact gene expression and product synthesis by changing the DNA methylation status of functional genes involved in secondary metabolite synthesis. However, further research is needed to fully understand the extent to which DNA methylation modifies the content of secondary metabolites to mediate plants’ responses to drought stress, as well as the underlying mechanisms involved. Our study found that in Eleutherococcus senticosus (E. senticosus), moderate water deprivation significantly decreased DNA methylation levels throughout the genome and at the promoters of EsFPS, EsSS, and EsSE. Transcription factors like EsMYB-r1, previously inhibited by DNA methylation, can re-bind to the EsFPS promotor region following DNA demethylation. This process promotes gene expression and, ultimately, saponin synthesis and accumulation. The increased saponin levels in E. senticosus acted as antioxidants, enhancing the plant’s adaptability to drought stress.https://doi.org/10.1186/s12864-024-10237-xDrought stressDNA methylationTranscription factorEleutherococcus senticosusSecondary metaboliteSaponin |
| spellingShingle | Shuo Wang XueLei Zhao Chang Li Jing Dong JiaCheng Ma YueHong Long ZhaoBin Xing DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress BMC Genomics Drought stress DNA methylation Transcription factor Eleutherococcus senticosus Secondary metabolite Saponin |
| title | DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress |
| title_full | DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress |
| title_fullStr | DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress |
| title_full_unstemmed | DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress |
| title_short | DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress |
| title_sort | dna methylation regulates the secondary metabolism of saponins to improve the adaptability of eleutherococcus senticosus during drought stress |
| topic | Drought stress DNA methylation Transcription factor Eleutherococcus senticosus Secondary metabolite Saponin |
| url | https://doi.org/10.1186/s12864-024-10237-x |
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