Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production
Abstract By cultivating a strain of Aspergillus tubingensis on agro-industrial by-products using solid-state fermentation technology, a biocatalyst containing more than 130 different enzymes was obtained. The enzymatic complex was composed mainly of hydrolases, among which a protease, an aspergillop...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-02-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-019-0241-0 |
| _version_ | 1823934928778166272 |
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| author | Anaïs Guillaume Aurore Thorigné Yoann Carré Joëlle Vinh Loïc Levavasseur |
| author_facet | Anaïs Guillaume Aurore Thorigné Yoann Carré Joëlle Vinh Loïc Levavasseur |
| author_sort | Anaïs Guillaume |
| collection | DOAJ |
| description | Abstract By cultivating a strain of Aspergillus tubingensis on agro-industrial by-products using solid-state fermentation technology, a biocatalyst containing more than 130 different enzymes was obtained. The enzymatic complex was composed mainly of hydrolases, among which a protease, an aspergillopepsin, accounted for more than half of the total proteins. Cell-wall-degrading enzymes such as pectinases, cellulases and hemicellulases were also highly represented. Adding the biocatalyst to corn mash at 1 kg/T corn allowed to significantly improve ethanol production performances. The final ethanol concentration was increased by 6.8% and the kinetics was accelerated by 14 h. The aim of this study was to identify the enzymes implicated in the effect on corn ethanol production. By fractionating the biocatalyst, the particular effect of the major enzymes was investigated. Experiments revealed that, together, the protease and two cellulolytic enzymes (an endoglucanase and a β-glucosidase) were responsible for 80% of the overall effect of the biocatalyst. Nevertheless, the crude extract of the biocatalyst showed greater impact than the combination of up to seven purified enzymes, demonstrating the complementary enzymatic complex obtained by solid-state fermentation. This technology could, therefore, be a relevant natural alternative to the use of GMO-derived enzymes in the ethanol industry. |
| first_indexed | 2024-12-16T22:27:25Z |
| format | Article |
| id | doaj.art-e8aef5498f614b85b5e6796a3d81730e |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-16T22:27:25Z |
| publishDate | 2019-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-e8aef5498f614b85b5e6796a3d81730e2022-12-21T22:13:50ZengSpringerOpenBioresources and Bioprocessing2197-43652019-02-016111210.1186/s40643-019-0241-0Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol productionAnaïs Guillaume0Aurore Thorigné1Yoann Carré2Joëlle Vinh3Loïc Levavasseur4Centre de Recherche et Innovation Soufflet, Ets J. Soufflet, Quai SarrailCentre de Recherche et Innovation Soufflet, Ets J. Soufflet, Quai SarrailCentre de Recherche et Innovation Soufflet, Ets J. Soufflet, Quai SarrailPSL University, ESPCI Paris (SMBP CNRS USR 3149)Centre de Recherche et Innovation Soufflet, Ets J. Soufflet, Quai SarrailAbstract By cultivating a strain of Aspergillus tubingensis on agro-industrial by-products using solid-state fermentation technology, a biocatalyst containing more than 130 different enzymes was obtained. The enzymatic complex was composed mainly of hydrolases, among which a protease, an aspergillopepsin, accounted for more than half of the total proteins. Cell-wall-degrading enzymes such as pectinases, cellulases and hemicellulases were also highly represented. Adding the biocatalyst to corn mash at 1 kg/T corn allowed to significantly improve ethanol production performances. The final ethanol concentration was increased by 6.8% and the kinetics was accelerated by 14 h. The aim of this study was to identify the enzymes implicated in the effect on corn ethanol production. By fractionating the biocatalyst, the particular effect of the major enzymes was investigated. Experiments revealed that, together, the protease and two cellulolytic enzymes (an endoglucanase and a β-glucosidase) were responsible for 80% of the overall effect of the biocatalyst. Nevertheless, the crude extract of the biocatalyst showed greater impact than the combination of up to seven purified enzymes, demonstrating the complementary enzymatic complex obtained by solid-state fermentation. This technology could, therefore, be a relevant natural alternative to the use of GMO-derived enzymes in the ethanol industry.http://link.springer.com/article/10.1186/s40643-019-0241-0Solid-state fermentationEnzymesProteaseCellulasesBioethanolCorn |
| spellingShingle | Anaïs Guillaume Aurore Thorigné Yoann Carré Joëlle Vinh Loïc Levavasseur Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production Bioresources and Bioprocessing Solid-state fermentation Enzymes Protease Cellulases Bioethanol Corn |
| title | Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production |
| title_full | Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production |
| title_fullStr | Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production |
| title_full_unstemmed | Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production |
| title_short | Contribution of proteases and cellulases produced by solid-state fermentation to the improvement of corn ethanol production |
| title_sort | contribution of proteases and cellulases produced by solid state fermentation to the improvement of corn ethanol production |
| topic | Solid-state fermentation Enzymes Protease Cellulases Bioethanol Corn |
| url | http://link.springer.com/article/10.1186/s40643-019-0241-0 |
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