Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling
Adipose tissue is a complex organ composed of various cell types and an extracellular matrix (ECM). The visceral adipose tissue (VAT) is dynamically altered in response to nutritional regimens that lead to local cues affecting the cells and ECM. The adipocytes are in conjunction with the surrounding...
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MDPI AG
2022-12-01
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author | Alex Naftaly Nadav Kislev Roza Izgilov Raizel Adler Michal Silber Ruth Shalgi Dafna Benayahu |
author_facet | Alex Naftaly Nadav Kislev Roza Izgilov Raizel Adler Michal Silber Ruth Shalgi Dafna Benayahu |
author_sort | Alex Naftaly |
collection | DOAJ |
description | Adipose tissue is a complex organ composed of various cell types and an extracellular matrix (ECM). The visceral adipose tissue (VAT) is dynamically altered in response to nutritional regimens that lead to local cues affecting the cells and ECM. The adipocytes are in conjunction with the surrounding ECM that maintains the tissue’s niche, provides a scaffold for cells and modulates their signaling. In this study, we provide a better understanding of the crosstalk between nutritional regimens and the ECM’s stiffness. Histological analyses showed that the adipocytes in mice fed a high-fat diet (HFD) were increased in size, while the ECM was also altered with changes in mass and composition. HFD-fed mice exhibited a decrease in elastin and an increase in collagenous proteins. Rheometer measurements revealed a stiffer ECM in whole tissue (nECM) and decellularized (deECM) in HFD-fed animals. These alterations in the ECM regulate cellular activity and influence their metabolic function. HFD-fed mice expressed high levels of the receptor for advanced-glycation-end-products (RAGE), indicating that AGEs might play a role in these processes. The cells also exhibited an increase in phosphoserine<sup>332</sup> of IRS-1, a decrease in the GLUT4 transporter levels at the cells’ membrane, and a consequent reduction in insulin sensitivity. These results show how alterations in the stiffness of ECM proteins can affect the mechanical cues transferred to adipocytes and, thereby, influence the adipocytes’ functionality, leading to metabolic disorders. |
first_indexed | 2024-03-09T17:45:01Z |
format | Article |
id | doaj.art-e8b1cf8690c5419ea7ebd8c5085c0cfd |
institution | Directory Open Access Journal |
issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-09T17:45:01Z |
publishDate | 2022-12-01 |
publisher | MDPI AG |
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series | International Journal of Molecular Sciences |
spelling | doaj.art-e8b1cf8690c5419ea7ebd8c5085c0cfd2023-11-24T11:15:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231523710.3390/ijms232315237Nutrition Alters the Stiffness of Adipose Tissue and Cell SignalingAlex Naftaly0Nadav Kislev1Roza Izgilov2Raizel Adler3Michal Silber4Ruth Shalgi5Dafna Benayahu6Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelAdipose tissue is a complex organ composed of various cell types and an extracellular matrix (ECM). The visceral adipose tissue (VAT) is dynamically altered in response to nutritional regimens that lead to local cues affecting the cells and ECM. The adipocytes are in conjunction with the surrounding ECM that maintains the tissue’s niche, provides a scaffold for cells and modulates their signaling. In this study, we provide a better understanding of the crosstalk between nutritional regimens and the ECM’s stiffness. Histological analyses showed that the adipocytes in mice fed a high-fat diet (HFD) were increased in size, while the ECM was also altered with changes in mass and composition. HFD-fed mice exhibited a decrease in elastin and an increase in collagenous proteins. Rheometer measurements revealed a stiffer ECM in whole tissue (nECM) and decellularized (deECM) in HFD-fed animals. These alterations in the ECM regulate cellular activity and influence their metabolic function. HFD-fed mice expressed high levels of the receptor for advanced-glycation-end-products (RAGE), indicating that AGEs might play a role in these processes. The cells also exhibited an increase in phosphoserine<sup>332</sup> of IRS-1, a decrease in the GLUT4 transporter levels at the cells’ membrane, and a consequent reduction in insulin sensitivity. These results show how alterations in the stiffness of ECM proteins can affect the mechanical cues transferred to adipocytes and, thereby, influence the adipocytes’ functionality, leading to metabolic disorders.https://www.mdpi.com/1422-0067/23/23/15237adipose tissuehigh-fat dietniche stiffnessAGE–RAGE |
spellingShingle | Alex Naftaly Nadav Kislev Roza Izgilov Raizel Adler Michal Silber Ruth Shalgi Dafna Benayahu Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling International Journal of Molecular Sciences adipose tissue high-fat diet niche stiffness AGE–RAGE |
title | Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling |
title_full | Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling |
title_fullStr | Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling |
title_full_unstemmed | Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling |
title_short | Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling |
title_sort | nutrition alters the stiffness of adipose tissue and cell signaling |
topic | adipose tissue high-fat diet niche stiffness AGE–RAGE |
url | https://www.mdpi.com/1422-0067/23/23/15237 |
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