Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta)
Abstract We investigated the proteomic profiles of two popcorn inbred lines, P2 (N-efficient and N-responsive) and L80 (N-inefficient and nonresponsive to N), under low (10% of N supply) and high (100% of N supply) nitrogen environments, associated with agronomic- and physiological-related traits to...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-05545-9 |
| _version_ | 1819231481231310848 |
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| author | Shahid Khan Vitor Batista Pinto Antônio Teixeira do Amaral Júnior Gabriel Moreno Bernardo Gonçalves Caio Cézar Guedes Corrêa Fernando Rafael Alves Ferreira Guilherme Augusto Rodrigues de Souza Eliemar Campostrini Marta Simone Mendonça Freitas Marlene Evangelista Vieira Talles de Oliveira Santos Valter Jário de Lima Samuel Henrique Kamphorst José Francisco Teixeira do Amaral Freddy Mora-Poblete Gonçalo Apolinário de Souza Filho Vanildo Silveira |
| author_facet | Shahid Khan Vitor Batista Pinto Antônio Teixeira do Amaral Júnior Gabriel Moreno Bernardo Gonçalves Caio Cézar Guedes Corrêa Fernando Rafael Alves Ferreira Guilherme Augusto Rodrigues de Souza Eliemar Campostrini Marta Simone Mendonça Freitas Marlene Evangelista Vieira Talles de Oliveira Santos Valter Jário de Lima Samuel Henrique Kamphorst José Francisco Teixeira do Amaral Freddy Mora-Poblete Gonçalo Apolinário de Souza Filho Vanildo Silveira |
| author_sort | Shahid Khan |
| collection | DOAJ |
| description | Abstract We investigated the proteomic profiles of two popcorn inbred lines, P2 (N-efficient and N-responsive) and L80 (N-inefficient and nonresponsive to N), under low (10% of N supply) and high (100% of N supply) nitrogen environments, associated with agronomic- and physiological-related traits to NUE. The comparative proteomic analysis allowed the identification of 79 differentially accumulated proteins (DAPs) in the comparison of high/low N for P2 and 96 DAPs in the comparison of high/low N for L80. The NUE and N uptake efficiency (NUpE) presented high means in P2 in comparison to L80 at both N levels, but the NUE, NUpE, and N utilization efficiency (NUtE) rates decreased in P2 under a high N supply. DAPs involved in energy and carbohydrate metabolism suggested that N regulates enzymes of alternative pathways to adapt to energy shortages and that fructose-bisphosphate aldolase may act as one of the key primary nitrate responsive proteins in P2. Proteins related to ascorbate biosynthesis and nitrogen metabolism increased their regulation in P2, and the interaction of l-ascorbate peroxidase and Fd-NiR may play an important role in the NUE trait. Taken together, our results provide new insights into the proteomic changes taking place in contrasting inbred lines, providing useful information on the genetic improvement of NUE in popcorn. |
| first_indexed | 2024-12-23T11:45:39Z |
| format | Article |
| id | doaj.art-e8b3b75c485847f499b45ce979e4b48e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T11:45:39Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e8b3b75c485847f499b45ce979e4b48e2022-12-21T17:48:21ZengNature PortfolioScientific Reports2045-23222022-01-0112111310.1038/s41598-022-05545-9Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta)Shahid Khan0Vitor Batista Pinto1Antônio Teixeira do Amaral Júnior2Gabriel Moreno Bernardo Gonçalves3Caio Cézar Guedes Corrêa4Fernando Rafael Alves Ferreira5Guilherme Augusto Rodrigues de Souza6Eliemar Campostrini7Marta Simone Mendonça Freitas8Marlene Evangelista Vieira9Talles de Oliveira Santos10Valter Jário de Lima11Samuel Henrique Kamphorst12José Francisco Teixeira do Amaral13Freddy Mora-Poblete14Gonçalo Apolinário de Souza Filho15Vanildo Silveira16Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Fitotecnia-Setor de Nutrição Mineral de Plantas, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal-Setor de Fisiologia Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal-Setor de Fisiologia Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Fitotecnia-Setor de Nutrição Mineral de Plantas, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Fitotecnia-Setor de Nutrição Mineral de Plantas, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Departamento de Engenharia Rural, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo (UFES)Instituto de Ciencias Biológicas, Universidad de TalcaLaboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Abstract We investigated the proteomic profiles of two popcorn inbred lines, P2 (N-efficient and N-responsive) and L80 (N-inefficient and nonresponsive to N), under low (10% of N supply) and high (100% of N supply) nitrogen environments, associated with agronomic- and physiological-related traits to NUE. The comparative proteomic analysis allowed the identification of 79 differentially accumulated proteins (DAPs) in the comparison of high/low N for P2 and 96 DAPs in the comparison of high/low N for L80. The NUE and N uptake efficiency (NUpE) presented high means in P2 in comparison to L80 at both N levels, but the NUE, NUpE, and N utilization efficiency (NUtE) rates decreased in P2 under a high N supply. DAPs involved in energy and carbohydrate metabolism suggested that N regulates enzymes of alternative pathways to adapt to energy shortages and that fructose-bisphosphate aldolase may act as one of the key primary nitrate responsive proteins in P2. Proteins related to ascorbate biosynthesis and nitrogen metabolism increased their regulation in P2, and the interaction of l-ascorbate peroxidase and Fd-NiR may play an important role in the NUE trait. Taken together, our results provide new insights into the proteomic changes taking place in contrasting inbred lines, providing useful information on the genetic improvement of NUE in popcorn.https://doi.org/10.1038/s41598-022-05545-9 |
| spellingShingle | Shahid Khan Vitor Batista Pinto Antônio Teixeira do Amaral Júnior Gabriel Moreno Bernardo Gonçalves Caio Cézar Guedes Corrêa Fernando Rafael Alves Ferreira Guilherme Augusto Rodrigues de Souza Eliemar Campostrini Marta Simone Mendonça Freitas Marlene Evangelista Vieira Talles de Oliveira Santos Valter Jário de Lima Samuel Henrique Kamphorst José Francisco Teixeira do Amaral Freddy Mora-Poblete Gonçalo Apolinário de Souza Filho Vanildo Silveira Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) Scientific Reports |
| title | Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) |
| title_full | Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) |
| title_fullStr | Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) |
| title_full_unstemmed | Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) |
| title_short | Revealing the differential protein profiles behind the nitrogen use efficiency in popcorn (Zea mays var. everta) |
| title_sort | revealing the differential protein profiles behind the nitrogen use efficiency in popcorn zea mays var everta |
| url | https://doi.org/10.1038/s41598-022-05545-9 |
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