Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation
Tissue culture independent transformation technique in crops is relatively new and of popular interest due to its faster approach and efficiency. The prospect of this technique in the production of transgenic Jute plants with new genetic properties is promising. In the present study, two varieties o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Plovdiv University Press
2015-08-01
|
| Series: | Journal of BioScience and Biotechnology |
| Subjects: | |
| Online Access: | http://www.jbb.uni-plovdiv.bg/documents/27807/1014561/jbb_2015-4(2)-pages_201-205.pdf |
| _version_ | 1818386654212128768 |
|---|---|
| author | REDUANUL BARI MD. ANIK ASHFAQ KHAN MOHAMMAD MAJHARUL ISLAM PIJUSH SUTRADHAR MD. JAHANGIR ALAM ASMA KHATUN |
| author_facet | REDUANUL BARI MD. ANIK ASHFAQ KHAN MOHAMMAD MAJHARUL ISLAM PIJUSH SUTRADHAR MD. JAHANGIR ALAM ASMA KHATUN |
| author_sort | REDUANUL BARI |
| collection | DOAJ |
| description | Tissue culture independent transformation technique in crops is relatively new and of popular interest due to its faster approach and efficiency. The prospect of this technique in the production of transgenic Jute plants with new genetic properties is promising. In the present study, two varieties of each of Corchorus olitorius (var. O-72 and var. OM-1) and C. capsularis (var. CVL-1 and var. BJC-83) were used to observe their transformation ability. Agrobacterium tumefaciens strain LBA4404 was used for transformation, which harbors a binary vector pBI121 containing selectable marker gene nptII, gus (β-glucuronidase) reporter gene and a stress tolerance gene GLY-1. The young shoot tip of the seedlings (20-22 cm) was infected with the bacterial culture. The young leaves were collected after 20-21 days of bacterial culture transformation and thereafter histochemical GUS assay was performed. In the putatively transformed regions of the plants, gus reporter gene was expressed showing blue color in the tissues. Non-transformed plants did not show any color. Among the varieties, the percentage of matured plants showing GUS activity was higher in C. olitorious var. O-72 (80.66%) and OM-1 (73.33%) compared to C. capsularis var. BJC-83 (32.50%) and CVL-1 (40.00%). The result of the study provides an indication that efficiency of transformation by using tissue culture independent direct genetic transformation for the two species of Jute may differ significantly. |
| first_indexed | 2024-12-14T03:57:29Z |
| format | Article |
| id | doaj.art-e8b4b53da30049859e8e8bc7353f6092 |
| institution | Directory Open Access Journal |
| issn | 1314-6238 1314-6246 |
| language | English |
| last_indexed | 2024-12-14T03:57:29Z |
| publishDate | 2015-08-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj.art-e8b4b53da30049859e8e8bc7353f60922022-12-21T23:18:03ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462015-08-0142201205Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformationREDUANUL BARI0MD. ANIK ASHFAQ KHAN1MOHAMMAD MAJHARUL ISLAM2PIJUSH SUTRADHAR3MD. JAHANGIR ALAM 4ASMA KHATUN5Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Genetic Resources and Seed Division, Bangladesh Jute Research Institute, Dhaka-1207, Bangladesh.Tissue culture independent transformation technique in crops is relatively new and of popular interest due to its faster approach and efficiency. The prospect of this technique in the production of transgenic Jute plants with new genetic properties is promising. In the present study, two varieties of each of Corchorus olitorius (var. O-72 and var. OM-1) and C. capsularis (var. CVL-1 and var. BJC-83) were used to observe their transformation ability. Agrobacterium tumefaciens strain LBA4404 was used for transformation, which harbors a binary vector pBI121 containing selectable marker gene nptII, gus (β-glucuronidase) reporter gene and a stress tolerance gene GLY-1. The young shoot tip of the seedlings (20-22 cm) was infected with the bacterial culture. The young leaves were collected after 20-21 days of bacterial culture transformation and thereafter histochemical GUS assay was performed. In the putatively transformed regions of the plants, gus reporter gene was expressed showing blue color in the tissues. Non-transformed plants did not show any color. Among the varieties, the percentage of matured plants showing GUS activity was higher in C. olitorious var. O-72 (80.66%) and OM-1 (73.33%) compared to C. capsularis var. BJC-83 (32.50%) and CVL-1 (40.00%). The result of the study provides an indication that efficiency of transformation by using tissue culture independent direct genetic transformation for the two species of Jute may differ significantly.http://www.jbb.uni-plovdiv.bg/documents/27807/1014561/jbb_2015-4(2)-pages_201-205.pdfCorchorus olitoriusCorchorus capsularisGUS assaytissue culture independent transformation |
| spellingShingle | REDUANUL BARI MD. ANIK ASHFAQ KHAN MOHAMMAD MAJHARUL ISLAM PIJUSH SUTRADHAR MD. JAHANGIR ALAM ASMA KHATUN Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation Journal of BioScience and Biotechnology Corchorus olitorius Corchorus capsularis GUS assay tissue culture independent transformation |
| title | Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation |
| title_full | Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation |
| title_fullStr | Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation |
| title_full_unstemmed | Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation |
| title_short | Comparison between Corchorus olitorius and Corchorus capsularis at GUS histochemical assay performance for tissue culture independent transformation |
| title_sort | comparison between corchorus olitorius and corchorus capsularis at gus histochemical assay performance for tissue culture independent transformation |
| topic | Corchorus olitorius Corchorus capsularis GUS assay tissue culture independent transformation |
| url | http://www.jbb.uni-plovdiv.bg/documents/27807/1014561/jbb_2015-4(2)-pages_201-205.pdf |
| work_keys_str_mv | AT reduanulbari comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation AT mdanikashfaqkhan comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation AT mohammadmajharulislam comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation AT pijushsutradhar comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation AT mdjahangiralam comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation AT asmakhatun comparisonbetweencorchorusolitoriusandcorchoruscapsularisatgushistochemicalassayperformancefortissuecultureindependenttransformation |