Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil
Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2019-03-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100180&lng=en&tlng=en |
| _version_ | 1818835271956824064 |
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| author | Andresa Guimarães Juliana Macedo Raimundo Raisa Braul Rodrigues Maristela Peckle Peixoto Huarrisson Azevedo Santos Marcos Rogério André Rosangela Zacarias Machado Cristiane Divan Baldani |
| author_facet | Andresa Guimarães Juliana Macedo Raimundo Raisa Braul Rodrigues Maristela Peckle Peixoto Huarrisson Azevedo Santos Marcos Rogério André Rosangela Zacarias Machado Cristiane Divan Baldani |
| author_sort | Andresa Guimarães |
| collection | DOAJ |
| description | Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms. |
| first_indexed | 2024-12-19T02:48:04Z |
| format | Article |
| id | doaj.art-e8b6c3051bbc4cfdb5b30ad57fabea02 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-19T02:48:04Z |
| publishDate | 2019-03-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-e8b6c3051bbc4cfdb5b30ad57fabea022022-12-21T20:38:46ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612019-03-0128118018510.1590/s1984-296120180088S1984-29612019000100180Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast BrazilAndresa GuimarãesJuliana Macedo RaimundoRaisa Braul RodriguesMaristela Peckle PeixotoHuarrisson Azevedo SantosMarcos Rogério AndréRosangela Zacarias MachadoCristiane Divan BaldaniAbstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100180&lng=en&tlng=enFelinenested PCRIFAhematologyehrlichiosis |
| spellingShingle | Andresa Guimarães Juliana Macedo Raimundo Raisa Braul Rodrigues Maristela Peckle Peixoto Huarrisson Azevedo Santos Marcos Rogério André Rosangela Zacarias Machado Cristiane Divan Baldani Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil Revista Brasileira de Parasitologia Veterinária Feline nested PCR IFA hematology ehrlichiosis |
| title | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
| title_full | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
| title_fullStr | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
| title_full_unstemmed | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
| title_short | Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil |
| title_sort | ehrlichia spp infection in domestic cats from rio de janeiro state southeast brazil |
| topic | Feline nested PCR IFA hematology ehrlichiosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100180&lng=en&tlng=en |
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