IGFN1_v1 is required for myoblast fusion and differentiation.
Igfn1 is a complex locus that codes for multiple splicing variants of Immunoglobulin- and Fibronectin-like domain containing proteins predominantly expressed in skeletal muscle. To reveal possible roles for Igfn1, we applied non-selective knock-down by shRNAs as well as specific targeting of Igfn1 e...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5493368?pdf=render |
| _version_ | 1818150531589210112 |
|---|---|
| author | Xiang Li Jane Baker Tobias Cracknell Andrew R Haynes Gonzalo Blanco |
| author_facet | Xiang Li Jane Baker Tobias Cracknell Andrew R Haynes Gonzalo Blanco |
| author_sort | Xiang Li |
| collection | DOAJ |
| description | Igfn1 is a complex locus that codes for multiple splicing variants of Immunoglobulin- and Fibronectin-like domain containing proteins predominantly expressed in skeletal muscle. To reveal possible roles for Igfn1, we applied non-selective knock-down by shRNAs as well as specific targeting of Igfn1 exon 13 by CRISPR/Cas9 mutagenesis in C2C12 cells. Decreased expression of Igfn1 variants via shRNAs against the common 3'-UTR region caused a total blunting of myoblast fusion, but did not prevent expression of differentiation markers. Targeting of N-terminal domains by elimination of exon 13 via CRISPR/Cas9 mediated homologous recombination, also resulted in fusion defects as well as large multinucleated cells. Expression of IGFN1_v1 partially rescued fusion and myotube morphology in the Igfn1 exon 13 knock-out cell line, indicating a role for this variant in myoblast fusion and differentiation. However, in vivo overexpression of IGFN1_v1 or the Igfn1 Exon 13 CRISPR/Cas9 targeting vector did not result in significant size changes in transfected fibres. |
| first_indexed | 2024-12-11T13:24:25Z |
| format | Article |
| id | doaj.art-e8b7aed2afa4422f81db83068dba2645 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T13:24:25Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e8b7aed2afa4422f81db83068dba26452022-12-22T01:05:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e018021710.1371/journal.pone.0180217IGFN1_v1 is required for myoblast fusion and differentiation.Xiang LiJane BakerTobias CracknellAndrew R HaynesGonzalo BlancoIgfn1 is a complex locus that codes for multiple splicing variants of Immunoglobulin- and Fibronectin-like domain containing proteins predominantly expressed in skeletal muscle. To reveal possible roles for Igfn1, we applied non-selective knock-down by shRNAs as well as specific targeting of Igfn1 exon 13 by CRISPR/Cas9 mutagenesis in C2C12 cells. Decreased expression of Igfn1 variants via shRNAs against the common 3'-UTR region caused a total blunting of myoblast fusion, but did not prevent expression of differentiation markers. Targeting of N-terminal domains by elimination of exon 13 via CRISPR/Cas9 mediated homologous recombination, also resulted in fusion defects as well as large multinucleated cells. Expression of IGFN1_v1 partially rescued fusion and myotube morphology in the Igfn1 exon 13 knock-out cell line, indicating a role for this variant in myoblast fusion and differentiation. However, in vivo overexpression of IGFN1_v1 or the Igfn1 Exon 13 CRISPR/Cas9 targeting vector did not result in significant size changes in transfected fibres.http://europepmc.org/articles/PMC5493368?pdf=render |
| spellingShingle | Xiang Li Jane Baker Tobias Cracknell Andrew R Haynes Gonzalo Blanco IGFN1_v1 is required for myoblast fusion and differentiation. PLoS ONE |
| title | IGFN1_v1 is required for myoblast fusion and differentiation. |
| title_full | IGFN1_v1 is required for myoblast fusion and differentiation. |
| title_fullStr | IGFN1_v1 is required for myoblast fusion and differentiation. |
| title_full_unstemmed | IGFN1_v1 is required for myoblast fusion and differentiation. |
| title_short | IGFN1_v1 is required for myoblast fusion and differentiation. |
| title_sort | igfn1 v1 is required for myoblast fusion and differentiation |
| url | http://europepmc.org/articles/PMC5493368?pdf=render |
| work_keys_str_mv | AT xiangli igfn1v1isrequiredformyoblastfusionanddifferentiation AT janebaker igfn1v1isrequiredformyoblastfusionanddifferentiation AT tobiascracknell igfn1v1isrequiredformyoblastfusionanddifferentiation AT andrewrhaynes igfn1v1isrequiredformyoblastfusionanddifferentiation AT gonzaloblanco igfn1v1isrequiredformyoblastfusionanddifferentiation |