The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress
Background/Aims: Naked mole rats (NMRs) survive and thrive in dark, dank environments with low levels of oxygen and poor quality nutrition. Their long lifespan is attributed to sustained good health and pronounced resistance to cancer. Physiological and biochemical processes, such as autophagy, may...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-07-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/363015 |
| _version_ | 1818876273993187328 |
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| author | Shanmin Zhao Hong Luo Guanghan Kan Yong Zhao Lifang Lin Qiu Tang Chenlin Yu Wei Sun Liping Cai Shufang Cui |
| author_facet | Shanmin Zhao Hong Luo Guanghan Kan Yong Zhao Lifang Lin Qiu Tang Chenlin Yu Wei Sun Liping Cai Shufang Cui |
| author_sort | Shanmin Zhao |
| collection | DOAJ |
| description | Background/Aims: Naked mole rats (NMRs) survive and thrive in dark, dank environments with low levels of oxygen and poor quality nutrition. Their long lifespan is attributed to sustained good health and pronounced resistance to cancer. Physiological and biochemical processes, such as autophagy, may contribute to the successful aging of this exceptionally long-lived species. We demonstrated that NMRs have higher levels of autophagy than short-lived C57BL/6 mice, and this may play an important role in the maintenance of cellular protein quality and the defense of cells against intracellular and extracellular aggressors in NMRs. The present study assesses autophagy as a means for cells to flexibly respond to environmental changes (H2O2 treatment and a shortage of nutrients). Methods: Primary NMR HSCs were isolated from liver and treated with serum-free medium. Cells in the experimental group were incubated with different concentrations of hydrogen peroxide (H2O2) in the presence and / or absence of 3-MA (5 mM).The LC3-II/LC3-I ratio was determined by western blot analysis. Western blotting was performed to analyze the expression level of Beclin 1 protein. Apoptosis and cell-cycle progression were analyzed by flow cytometry. Results: Our data reveal that both poor quality nutrition and H2O2 treatment induces apoptosis and autophagy in NMR hepatic stellate cells(HSCs). Conclusion: NMR cells have the capacity to induce cell death through apoptosis and downregulate the energy consuming processes through inhibition of proliferation when they become superfluous or irreversibly damaged. |
| first_indexed | 2024-12-19T13:39:47Z |
| format | Article |
| id | doaj.art-e8bb4690557344f29985deb8b9ae7be9 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-19T13:39:47Z |
| publishDate | 2014-07-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-e8bb4690557344f29985deb8b9ae7be92022-12-21T20:19:02ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-07-0134246347310.1159/000363015363015The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional StressShanmin ZhaoHong LuoGuanghan KanYong ZhaoLifang LinQiu TangChenlin YuWei SunLiping CaiShufang CuiBackground/Aims: Naked mole rats (NMRs) survive and thrive in dark, dank environments with low levels of oxygen and poor quality nutrition. Their long lifespan is attributed to sustained good health and pronounced resistance to cancer. Physiological and biochemical processes, such as autophagy, may contribute to the successful aging of this exceptionally long-lived species. We demonstrated that NMRs have higher levels of autophagy than short-lived C57BL/6 mice, and this may play an important role in the maintenance of cellular protein quality and the defense of cells against intracellular and extracellular aggressors in NMRs. The present study assesses autophagy as a means for cells to flexibly respond to environmental changes (H2O2 treatment and a shortage of nutrients). Methods: Primary NMR HSCs were isolated from liver and treated with serum-free medium. Cells in the experimental group were incubated with different concentrations of hydrogen peroxide (H2O2) in the presence and / or absence of 3-MA (5 mM).The LC3-II/LC3-I ratio was determined by western blot analysis. Western blotting was performed to analyze the expression level of Beclin 1 protein. Apoptosis and cell-cycle progression were analyzed by flow cytometry. Results: Our data reveal that both poor quality nutrition and H2O2 treatment induces apoptosis and autophagy in NMR hepatic stellate cells(HSCs). Conclusion: NMR cells have the capacity to induce cell death through apoptosis and downregulate the energy consuming processes through inhibition of proliferation when they become superfluous or irreversibly damaged.http://www.karger.com/Article/FullText/363015H2O2Nutritional StressHeterocephalus glaberHepatic Stellate CellsAutophagy |
| spellingShingle | Shanmin Zhao Hong Luo Guanghan Kan Yong Zhao Lifang Lin Qiu Tang Chenlin Yu Wei Sun Liping Cai Shufang Cui The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress Cellular Physiology and Biochemistry H2O2 Nutritional Stress Heterocephalus glaber Hepatic Stellate Cells Autophagy |
| title | The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress |
| title_full | The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress |
| title_fullStr | The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress |
| title_full_unstemmed | The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress |
| title_short | The Protective Role of Autophagy in Heterocephalus glaber Hepatic Stellate Cells Exposed to H2O2 or Nutritional Stress |
| title_sort | protective role of autophagy in heterocephalus glaber hepatic stellate cells exposed to h2o2 or nutritional stress |
| topic | H2O2 Nutritional Stress Heterocephalus glaber Hepatic Stellate Cells Autophagy |
| url | http://www.karger.com/Article/FullText/363015 |
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