Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell
AIM: To explore the effects and underlying mechanisms of glucocorticoid in the apoptosis of retinal ganglion cell(RGC).<p>METHODS: RGC were cultured in 4 groups for 3d: control group, glucocorticoid group(with 0.1μmol/L cortisone), glucocorticoid-siNgR group \〖with 0.1μmol/L cortisone+Nogo rec...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-06-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2015/6/201506009.pdf |
| _version_ | 1818912071831519232 |
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| author | Li-Zheng Lü Xue-Zheng Liu |
| author_facet | Li-Zheng Lü Xue-Zheng Liu |
| author_sort | Li-Zheng Lü |
| collection | DOAJ |
| description | AIM: To explore the effects and underlying mechanisms of glucocorticoid in the apoptosis of retinal ganglion cell(RGC).<p>METHODS: RGC were cultured in 4 groups for 3d: control group, glucocorticoid group(with 0.1μmol/L cortisone), glucocorticoid-siNgR group \〖with 0.1μmol/L cortisone+Nogo receptor(NgR)antisense nucleotide\〗, glucocorticoid-scRNA group(with 0.1μmol/L cortisone + scrambled nucleotide). The cell viability was detected by thiazolyl blue tetrazolium bromide(MTT), the morphological features were observed with inverted microscope, apoptosis of RGC was measured with Hoechst 33342 staining, and expression of NgR was revealed by Western blot. <p>RESULTS: Cell viability in control, glucocorticoid, glucocorticoid-scRNA and glucocorticoid-siNgR groups were(100.0±0.0)%,(76.3±6.8)%,(79.4±9.0)% and(96.7±9.8)% respectively. Decreased cell viability, reduced cell number, and increased expression of NgR were atrophic cell body detected in glucocorticoid and glucocorticoid-scRNA groups(<i>P</i><0.01), not in glucocorticoid-siNgR group(<i>P</i>>0.05), compared with control group. RGC was showed light blue by Hoechst 33 342 staining in control and glucocorticoid-siNgR groups, and exhibiting bright blued apoptotic RGC in glucocorticoid and glucocorticoid-scRNA groups.<p>CONCLUSION: Up-regulation of NgR contributes to glucocorticoids-induced apoptosis of RGC. |
| first_indexed | 2024-12-19T23:08:46Z |
| format | Article |
| id | doaj.art-e8bce37a15784ab7a4fdf11481f3860d |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-19T23:08:46Z |
| publishDate | 2015-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-e8bce37a15784ab7a4fdf11481f3860d2022-12-21T20:02:18ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232015-06-0115697697810.3980/j.issn.1672-5123.2015.6.09Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cellLi-Zheng Lü0Xue-Zheng Liu1Department of Anthropotomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, ChinaDepartment of Anthropotomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, ChinaAIM: To explore the effects and underlying mechanisms of glucocorticoid in the apoptosis of retinal ganglion cell(RGC).<p>METHODS: RGC were cultured in 4 groups for 3d: control group, glucocorticoid group(with 0.1μmol/L cortisone), glucocorticoid-siNgR group \〖with 0.1μmol/L cortisone+Nogo receptor(NgR)antisense nucleotide\〗, glucocorticoid-scRNA group(with 0.1μmol/L cortisone + scrambled nucleotide). The cell viability was detected by thiazolyl blue tetrazolium bromide(MTT), the morphological features were observed with inverted microscope, apoptosis of RGC was measured with Hoechst 33342 staining, and expression of NgR was revealed by Western blot. <p>RESULTS: Cell viability in control, glucocorticoid, glucocorticoid-scRNA and glucocorticoid-siNgR groups were(100.0±0.0)%,(76.3±6.8)%,(79.4±9.0)% and(96.7±9.8)% respectively. Decreased cell viability, reduced cell number, and increased expression of NgR were atrophic cell body detected in glucocorticoid and glucocorticoid-scRNA groups(<i>P</i><0.01), not in glucocorticoid-siNgR group(<i>P</i>>0.05), compared with control group. RGC was showed light blue by Hoechst 33 342 staining in control and glucocorticoid-siNgR groups, and exhibiting bright blued apoptotic RGC in glucocorticoid and glucocorticoid-scRNA groups.<p>CONCLUSION: Up-regulation of NgR contributes to glucocorticoids-induced apoptosis of RGC.http://ies.ijo.cn/cn_publish/2015/6/201506009.pdfglucocorticoidNogo receptorretinal ganglion cellapoptosis |
| spellingShingle | Li-Zheng Lü Xue-Zheng Liu Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell Guoji Yanke Zazhi glucocorticoid Nogo receptor retinal ganglion cell apoptosis |
| title | Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell |
| title_full | Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell |
| title_fullStr | Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell |
| title_full_unstemmed | Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell |
| title_short | Role of Nogo receptor in glucocorticoids-induced apoptosis of retinal ganglion cell |
| title_sort | role of nogo receptor in glucocorticoids induced apoptosis of retinal ganglion cell |
| topic | glucocorticoid Nogo receptor retinal ganglion cell apoptosis |
| url | http://ies.ijo.cn/cn_publish/2015/6/201506009.pdf |
| work_keys_str_mv | AT lizhenglu roleofnogoreceptoringlucocorticoidsinducedapoptosisofretinalganglioncell AT xuezhengliu roleofnogoreceptoringlucocorticoidsinducedapoptosisofretinalganglioncell |