Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice
Abstract Background Premature ovarian insufficiency (POI) is characterized by early loss of ovarian function that affects women before the age of 40. We aim to explore the protective effects of transcutaneous electrical acupoint stimulation (TEAS) against irradiation-induced ovarian damage in mice....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-07-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-019-0541-1 |
| _version_ | 1797970143742001152 |
|---|---|
| author | Rongrong Tan Yuheng He Suyun Zhang Danhua Pu Jie Wu |
| author_facet | Rongrong Tan Yuheng He Suyun Zhang Danhua Pu Jie Wu |
| author_sort | Rongrong Tan |
| collection | DOAJ |
| description | Abstract Background Premature ovarian insufficiency (POI) is characterized by early loss of ovarian function that affects women before the age of 40. We aim to explore the protective effects of transcutaneous electrical acupoint stimulation (TEAS) against irradiation-induced ovarian damage in mice. Methods C57BL6 mice were randomly divided into control and irradiation (IR) groups. Then, control group was divided into two treatment subgroups: mock TEAS treatment (control-) and TEAS treatment (control+). IR group was divided into four subgroups according to the time of treatment started: mock TEAS treatment initiated at 2 days after irradiation (IR 2D-), TEAS treatment initiated at 2 days after irradiation (IR 2D+), mock TEAS treatment initiated at 1 week after irradiation (IR 1 W-), and TEAS treatment initiated at 1 week after irradiation (IR 1 W+). The radiation model mice were exposed to single whole body X-ray irradiation (4 Gy), and the control mice received 0 Gy. TEAS stimulation (2 Hz, 1 mA, 30 min/day) was given once a day for six consecutive days per week for 2 weeks. Estrous cycle, ovarian weight, serum AMH level and follicle counts were evaluated. Then, proliferation markers, apoptotic markers and oxidative stress markers were examined. Results Compared with the control group, the estrous cycle was disordered, and the ovarian weight, serum AMH, and primordial, primary and secondary follicles counts decreased (all P < 0.01) in the IR 2D- and IR 1 W- groups. In the irradiation with early TEAS treatment group (IR 2D+), the estrous cycle improved, the AMH level and primordial follicular significantly increased compared to the irradiation with mock group (IR 2D-). However, there were no significant differences in the estrous cycle, AMH level and follicle counts between IR 1 W- and IR 1 W+ groups. Moreover, IR 2D+ mice reduced the expression of Bax protein and increased the levels of Bcl-2 and PCNA compared to the IR 2D- group. Furthermore, the early TEAS treated mice showed significantly lower levels of oxidative stress and number of TUNEL (+) granulosa cells than that in the IR 2D- group. Conclusion This study is first to evaluate TEAS as a potential therapy to attenuate irradiation-induced ovarian failure through inhibiting primordial follicles loss, increasing serum AMH secretion, inducing antioxidant, and anti-apoptotic systems. |
| first_indexed | 2024-04-11T03:13:18Z |
| format | Article |
| id | doaj.art-e8bd79d36a314aa6a698ec00cf2ffd92 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:13:18Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-e8bd79d36a314aa6a698ec00cf2ffd922023-01-02T11:20:04ZengBMCJournal of Ovarian Research1757-22152019-07-0112111110.1186/s13048-019-0541-1Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in miceRongrong Tan0Yuheng He1Suyun Zhang2Danhua Pu3Jie Wu4State Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical UniversityAbstract Background Premature ovarian insufficiency (POI) is characterized by early loss of ovarian function that affects women before the age of 40. We aim to explore the protective effects of transcutaneous electrical acupoint stimulation (TEAS) against irradiation-induced ovarian damage in mice. Methods C57BL6 mice were randomly divided into control and irradiation (IR) groups. Then, control group was divided into two treatment subgroups: mock TEAS treatment (control-) and TEAS treatment (control+). IR group was divided into four subgroups according to the time of treatment started: mock TEAS treatment initiated at 2 days after irradiation (IR 2D-), TEAS treatment initiated at 2 days after irradiation (IR 2D+), mock TEAS treatment initiated at 1 week after irradiation (IR 1 W-), and TEAS treatment initiated at 1 week after irradiation (IR 1 W+). The radiation model mice were exposed to single whole body X-ray irradiation (4 Gy), and the control mice received 0 Gy. TEAS stimulation (2 Hz, 1 mA, 30 min/day) was given once a day for six consecutive days per week for 2 weeks. Estrous cycle, ovarian weight, serum AMH level and follicle counts were evaluated. Then, proliferation markers, apoptotic markers and oxidative stress markers were examined. Results Compared with the control group, the estrous cycle was disordered, and the ovarian weight, serum AMH, and primordial, primary and secondary follicles counts decreased (all P < 0.01) in the IR 2D- and IR 1 W- groups. In the irradiation with early TEAS treatment group (IR 2D+), the estrous cycle improved, the AMH level and primordial follicular significantly increased compared to the irradiation with mock group (IR 2D-). However, there were no significant differences in the estrous cycle, AMH level and follicle counts between IR 1 W- and IR 1 W+ groups. Moreover, IR 2D+ mice reduced the expression of Bax protein and increased the levels of Bcl-2 and PCNA compared to the IR 2D- group. Furthermore, the early TEAS treated mice showed significantly lower levels of oxidative stress and number of TUNEL (+) granulosa cells than that in the IR 2D- group. Conclusion This study is first to evaluate TEAS as a potential therapy to attenuate irradiation-induced ovarian failure through inhibiting primordial follicles loss, increasing serum AMH secretion, inducing antioxidant, and anti-apoptotic systems.http://link.springer.com/article/10.1186/s13048-019-0541-1Irradiation protectionTranscutaneous electrical acupoint stimulation (TEAS)Ovarian damage |
| spellingShingle | Rongrong Tan Yuheng He Suyun Zhang Danhua Pu Jie Wu Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice Journal of Ovarian Research Irradiation protection Transcutaneous electrical acupoint stimulation (TEAS) Ovarian damage |
| title | Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice |
| title_full | Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice |
| title_fullStr | Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice |
| title_full_unstemmed | Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice |
| title_short | Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice |
| title_sort | effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy induced ovarian damage in mice |
| topic | Irradiation protection Transcutaneous electrical acupoint stimulation (TEAS) Ovarian damage |
| url | http://link.springer.com/article/10.1186/s13048-019-0541-1 |
| work_keys_str_mv | AT rongrongtan effectoftranscutaneouselectricalacupointstimulationonprotectingagainstradiotherapyinducedovariandamageinmice AT yuhenghe effectoftranscutaneouselectricalacupointstimulationonprotectingagainstradiotherapyinducedovariandamageinmice AT suyunzhang effectoftranscutaneouselectricalacupointstimulationonprotectingagainstradiotherapyinducedovariandamageinmice AT danhuapu effectoftranscutaneouselectricalacupointstimulationonprotectingagainstradiotherapyinducedovariandamageinmice AT jiewu effectoftranscutaneouselectricalacupointstimulationonprotectingagainstradiotherapyinducedovariandamageinmice |