Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function
It is essential to understand the ultrasound-induced changes in assembly of proteins and polyphenols into non covalent nanocomplex. β-Lactoglobulin (LG) and chlorogenic acid (CA) with various biological activities can be combined to form food-grade nanocomplexes. This study systematically explored t...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417722001183 |
| _version_ | 1811258748417605632 |
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| author | Jiayuan Liu Gongshuai Song Yawen Yuan Like Zhou Danli Wang Tinglan Yuan Ling Li Guanghua He Qingyu Yang Gongnian Xiao Jinyan Gong |
| author_facet | Jiayuan Liu Gongshuai Song Yawen Yuan Like Zhou Danli Wang Tinglan Yuan Ling Li Guanghua He Qingyu Yang Gongnian Xiao Jinyan Gong |
| author_sort | Jiayuan Liu |
| collection | DOAJ |
| description | It is essential to understand the ultrasound-induced changes in assembly of proteins and polyphenols into non covalent nanocomplex. β-Lactoglobulin (LG) and chlorogenic acid (CA) with various biological activities can be combined to form food-grade nanocomplexes. This study systematically explored the role of high-intensity ultrasound pretreatment on the binding mechanisms of LG and CA, and the potential biological function for embedding curcumin (Cur). The scanning electron microscopy (SEM) revealed that ultrasound treatment could destroy the structure of LG, and the particle size of the protein was reduced to<50 nm. The change in secondary structure of the protein by ultrasound treatment could be revealed by the fourier transform infrared (FTIR) and fluorescence spectra. Besides, it was found that LG and CA were combined to form a complex under the hydrophobic interaction, and CA was bound in the internal cavity of LG with a relatively extended conformation. The result demonstrated that the ratio of Cur embedded in the ultrasonic sample could be effectively increased by 7% − 10%, the particle size in the emulsion was smaller, and the dispersion was more stable. This work contributes to the development of protein–polyphenol functional emulsion systems with the ability to deliver Cur. |
| first_indexed | 2024-04-12T18:19:16Z |
| format | Article |
| id | doaj.art-e8bd9a2dcf114b3682e2feea0f5b68d5 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-04-12T18:19:16Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-e8bd9a2dcf114b3682e2feea0f5b68d52022-12-22T03:21:30ZengElsevierUltrasonics Sonochemistry1350-41772022-05-0186106025Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological functionJiayuan Liu0Gongshuai Song1Yawen Yuan2Like Zhou3Danli Wang4Tinglan Yuan5Ling Li6Guanghua He7Qingyu Yang8Gongnian Xiao9Jinyan Gong10Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaCollege of Grain Science and Technology, Shenyang Normal University, Shenyang 110034, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, ChinaZhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China; Corresponding author.It is essential to understand the ultrasound-induced changes in assembly of proteins and polyphenols into non covalent nanocomplex. β-Lactoglobulin (LG) and chlorogenic acid (CA) with various biological activities can be combined to form food-grade nanocomplexes. This study systematically explored the role of high-intensity ultrasound pretreatment on the binding mechanisms of LG and CA, and the potential biological function for embedding curcumin (Cur). The scanning electron microscopy (SEM) revealed that ultrasound treatment could destroy the structure of LG, and the particle size of the protein was reduced to<50 nm. The change in secondary structure of the protein by ultrasound treatment could be revealed by the fourier transform infrared (FTIR) and fluorescence spectra. Besides, it was found that LG and CA were combined to form a complex under the hydrophobic interaction, and CA was bound in the internal cavity of LG with a relatively extended conformation. The result demonstrated that the ratio of Cur embedded in the ultrasonic sample could be effectively increased by 7% − 10%, the particle size in the emulsion was smaller, and the dispersion was more stable. This work contributes to the development of protein–polyphenol functional emulsion systems with the ability to deliver Cur.http://www.sciencedirect.com/science/article/pii/S1350417722001183β- LactoglobulinChlorogenic acidCurcuminUltrasound |
| spellingShingle | Jiayuan Liu Gongshuai Song Yawen Yuan Like Zhou Danli Wang Tinglan Yuan Ling Li Guanghua He Qingyu Yang Gongnian Xiao Jinyan Gong Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function Ultrasonics Sonochemistry β- Lactoglobulin Chlorogenic acid Curcumin Ultrasound |
| title | Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function |
| title_full | Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function |
| title_fullStr | Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function |
| title_full_unstemmed | Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function |
| title_short | Ultrasound-assisted assembly of β-lactoglobulin and chlorogenic acid for non covalent nanocomplex: fabrication, characterization and potential biological function |
| title_sort | ultrasound assisted assembly of β lactoglobulin and chlorogenic acid for non covalent nanocomplex fabrication characterization and potential biological function |
| topic | β- Lactoglobulin Chlorogenic acid Curcumin Ultrasound |
| url | http://www.sciencedirect.com/science/article/pii/S1350417722001183 |
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