In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell
After anastomosis of sutures or pins, the restoration of intestinal barrier function can avoid several complications, such as tissue damage and inflammation. Our previous studies demonstrated the feasibility of biodegradable magnesium (Mg) pins as novel anastomosing implants to spontaneously absorb...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-04-01
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| Series: | Journal of Applied Biomaterials & Functional Materials |
| Online Access: | https://doi.org/10.1177/22808000231165281 |
| _version_ | 1797844723910574080 |
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| author | Ting Shan Jun Yan Xiaonong Zhang Yigang Chen |
| author_facet | Ting Shan Jun Yan Xiaonong Zhang Yigang Chen |
| author_sort | Ting Shan |
| collection | DOAJ |
| description | After anastomosis of sutures or pins, the restoration of intestinal barrier function can avoid several complications, such as tissue damage and inflammation. Our previous studies demonstrated the feasibility of biodegradable magnesium (Mg) pins as novel anastomosing implants to spontaneously absorb in the body, avoiding secondary removal surgery and long-term inflammation. However, the effect of Mg pins on the intestinal tight junction barrier is rarely investigated. In this study, we conducted high-purity Mg pins inserted in the intestine of rats and prepared Mg extracts cultured intestinal epithelial cell line to investigate the biological effect on the intestinal barrier associated with tight junction protein expression. We discovered that the concentration of released Mg ions over 1.7 mM was the critical threshold, above which mRNA expression of intestinal tight junction and cell apoptosis were affected considerably. Results of the immunohistochemical analysis revealed that Mg functions to stimulate ZO-1, caspase-3, occluding, and claudin-3 expressions. We offer new insight into the effectiveness of biodegradable Mg materials as the next generation of intestinal anastomosis pins, which effectively filters toxins as well as bacteria, and reduces inflammation. |
| first_indexed | 2024-04-09T17:27:37Z |
| format | Article |
| id | doaj.art-e8bf1dbd893d4a03ac67be4cbd61d756 |
| institution | Directory Open Access Journal |
| issn | 2280-8000 |
| language | English |
| last_indexed | 2024-04-09T17:27:37Z |
| publishDate | 2023-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Applied Biomaterials & Functional Materials |
| spelling | doaj.art-e8bf1dbd893d4a03ac67be4cbd61d7562023-04-18T11:03:57ZengSAGE PublishingJournal of Applied Biomaterials & Functional Materials2280-80002023-04-012110.1177/22808000231165281In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cellTing Shan0Jun Yan1Xiaonong Zhang2Yigang Chen3Department of General Surgery, Affiliated Wuxi Clinical College of Nantong University, Jiangsu, ChinaDepartment of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaState Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of General Surgery, Affiliated Wuxi Clinical College of Nantong University, Jiangsu, ChinaAfter anastomosis of sutures or pins, the restoration of intestinal barrier function can avoid several complications, such as tissue damage and inflammation. Our previous studies demonstrated the feasibility of biodegradable magnesium (Mg) pins as novel anastomosing implants to spontaneously absorb in the body, avoiding secondary removal surgery and long-term inflammation. However, the effect of Mg pins on the intestinal tight junction barrier is rarely investigated. In this study, we conducted high-purity Mg pins inserted in the intestine of rats and prepared Mg extracts cultured intestinal epithelial cell line to investigate the biological effect on the intestinal barrier associated with tight junction protein expression. We discovered that the concentration of released Mg ions over 1.7 mM was the critical threshold, above which mRNA expression of intestinal tight junction and cell apoptosis were affected considerably. Results of the immunohistochemical analysis revealed that Mg functions to stimulate ZO-1, caspase-3, occluding, and claudin-3 expressions. We offer new insight into the effectiveness of biodegradable Mg materials as the next generation of intestinal anastomosis pins, which effectively filters toxins as well as bacteria, and reduces inflammation.https://doi.org/10.1177/22808000231165281 |
| spellingShingle | Ting Shan Jun Yan Xiaonong Zhang Yigang Chen In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell Journal of Applied Biomaterials & Functional Materials |
| title | In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell |
| title_full | In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell |
| title_fullStr | In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell |
| title_full_unstemmed | In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell |
| title_short | In vitro and in vivo evaluation of effects of high-purity magnesium on tight junction of intestinal epithelial cell |
| title_sort | in vitro and in vivo evaluation of effects of high purity magnesium on tight junction of intestinal epithelial cell |
| url | https://doi.org/10.1177/22808000231165281 |
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