Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats
Hypercholesterolemia can cause many diseases, but it can effectively regulated by <i>Lactobacillus</i>. This study aimed to evaluate the cholesterol-lowering mechanism of <i>Enterococcus faecium</i> strain 132 and <i>Lactobacillus</i><i>paracasei</i> s...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2072-6643/13/6/1982 |
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| author | Lingshuang Yang Xinqiang Xie Ying Li Lei Wu Congcong Fan Tingting Liang Yu Xi Shuanghong Yang Haixin Li Jumei Zhang Yu Ding Liang Xue Moutong Chen Juan Wang Qingping Wu |
| author_facet | Lingshuang Yang Xinqiang Xie Ying Li Lei Wu Congcong Fan Tingting Liang Yu Xi Shuanghong Yang Haixin Li Jumei Zhang Yu Ding Liang Xue Moutong Chen Juan Wang Qingping Wu |
| author_sort | Lingshuang Yang |
| collection | DOAJ |
| description | Hypercholesterolemia can cause many diseases, but it can effectively regulated by <i>Lactobacillus</i>. This study aimed to evaluate the cholesterol-lowering mechanism of <i>Enterococcus faecium</i> strain 132 and <i>Lactobacillus</i><i>paracasei</i> strain 201. These results showed that both the strains decreased serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), liver TC and TG and increased fecal TC, TG and total bile acid (TBA) levels. Additionally, both strains also reduced glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST) and levels of tissue inflammation levels to improve the lipid profile, and they reduced fat accumulation partially by alleviating inflammatory responses. Furthermore, both strains regulated the expression of the <i>CYP8B1</i>, <i>CYP7A1, SREBP-1</i>, <i>SCD1</i> and <i>LDL-R</i> gene to promote cholesterol metabolism and reduce TG accumulation. Interventions with both strains also altered the gut microbiota, and decreasing the abundance of Veillonellaceae, Erysipelotrichaceae and <i>Prevotella</i>. Furthermore, fecal acetic acid and propionic acid were increased by this intervention. Overall, the results suggested that <i>E. faecium</i> strain 132 and <i>L. paracasei</i> strain 201 can alleviate hypercholesterolemia in rats and might be applied as a new type of hypercholesterolemia agent in functional foods. |
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| series | Nutrients |
| spelling | doaj.art-e8c27ee7538547e1bc9e9ff6bfb940902023-11-21T23:21:28ZengMDPI AGNutrients2072-66432021-06-01136198210.3390/nu13061982Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia RatsLingshuang Yang0Xinqiang Xie1Ying Li2Lei Wu3Congcong Fan4Tingting Liang5Yu Xi6Shuanghong Yang7Haixin Li8Jumei Zhang9Yu Ding10Liang Xue11Moutong Chen12Juan Wang13Qingping Wu14College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaHypercholesterolemia can cause many diseases, but it can effectively regulated by <i>Lactobacillus</i>. This study aimed to evaluate the cholesterol-lowering mechanism of <i>Enterococcus faecium</i> strain 132 and <i>Lactobacillus</i><i>paracasei</i> strain 201. These results showed that both the strains decreased serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), liver TC and TG and increased fecal TC, TG and total bile acid (TBA) levels. Additionally, both strains also reduced glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST) and levels of tissue inflammation levels to improve the lipid profile, and they reduced fat accumulation partially by alleviating inflammatory responses. Furthermore, both strains regulated the expression of the <i>CYP8B1</i>, <i>CYP7A1, SREBP-1</i>, <i>SCD1</i> and <i>LDL-R</i> gene to promote cholesterol metabolism and reduce TG accumulation. Interventions with both strains also altered the gut microbiota, and decreasing the abundance of Veillonellaceae, Erysipelotrichaceae and <i>Prevotella</i>. Furthermore, fecal acetic acid and propionic acid were increased by this intervention. Overall, the results suggested that <i>E. faecium</i> strain 132 and <i>L. paracasei</i> strain 201 can alleviate hypercholesterolemia in rats and might be applied as a new type of hypercholesterolemia agent in functional foods.https://www.mdpi.com/2072-6643/13/6/1982cholesterol-lowering<i>Enterococcus faecium</i> strain 132<i>Lactobacillus paracasei</i> strain 201gut microbiota |
| spellingShingle | Lingshuang Yang Xinqiang Xie Ying Li Lei Wu Congcong Fan Tingting Liang Yu Xi Shuanghong Yang Haixin Li Jumei Zhang Yu Ding Liang Xue Moutong Chen Juan Wang Qingping Wu Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats Nutrients cholesterol-lowering <i>Enterococcus faecium</i> strain 132 <i>Lactobacillus paracasei</i> strain 201 gut microbiota |
| title | Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats |
| title_full | Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats |
| title_fullStr | Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats |
| title_full_unstemmed | Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats |
| title_short | Evaluation of the Cholesterol-Lowering Mechanism of <i>Enterococcus faecium</i> Strain 132 and <i>Lactobacillus paracasei</i> Strain 201 in Hypercholesterolemia Rats |
| title_sort | evaluation of the cholesterol lowering mechanism of i enterococcus faecium i strain 132 and i lactobacillus paracasei i strain 201 in hypercholesterolemia rats |
| topic | cholesterol-lowering <i>Enterococcus faecium</i> strain 132 <i>Lactobacillus paracasei</i> strain 201 gut microbiota |
| url | https://www.mdpi.com/2072-6643/13/6/1982 |
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