Selected In Situ Hybridization Methods: Principles and Application
We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complement...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/13/3874 |
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| author | Dominika Veselinyová Jana Mašlanková Katarina Kalinová Helena Mičková Mária Mareková Miroslava Rabajdová |
| author_facet | Dominika Veselinyová Jana Mašlanková Katarina Kalinová Helena Mičková Mária Mareková Miroslava Rabajdová |
| author_sort | Dominika Veselinyová |
| collection | DOAJ |
| description | We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level. |
| first_indexed | 2024-03-10T10:05:05Z |
| format | Article |
| id | doaj.art-e8c34cb063f24f2da8278e7c670949ba |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:05:05Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e8c34cb063f24f2da8278e7c670949ba2023-11-22T01:39:25ZengMDPI AGMolecules1420-30492021-06-012613387410.3390/molecules26133874Selected In Situ Hybridization Methods: Principles and ApplicationDominika Veselinyová0Jana Mašlanková1Katarina Kalinová2Helena Mičková3Mária Mareková4Miroslava Rabajdová5Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaDepartment of Medical Biology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, SlovakiaWe are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level.https://www.mdpi.com/1420-3049/26/13/3874fluorescent in situ hybridizationDNA/RNA imagingapplication of FISH |
| spellingShingle | Dominika Veselinyová Jana Mašlanková Katarina Kalinová Helena Mičková Mária Mareková Miroslava Rabajdová Selected In Situ Hybridization Methods: Principles and Application Molecules fluorescent in situ hybridization DNA/RNA imaging application of FISH |
| title | Selected In Situ Hybridization Methods: Principles and Application |
| title_full | Selected In Situ Hybridization Methods: Principles and Application |
| title_fullStr | Selected In Situ Hybridization Methods: Principles and Application |
| title_full_unstemmed | Selected In Situ Hybridization Methods: Principles and Application |
| title_short | Selected In Situ Hybridization Methods: Principles and Application |
| title_sort | selected in situ hybridization methods principles and application |
| topic | fluorescent in situ hybridization DNA/RNA imaging application of FISH |
| url | https://www.mdpi.com/1420-3049/26/13/3874 |
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