Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants
In order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water succ...
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MDPI AG
2023-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/14/2652 |
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| author | Na Wang Haiwei Pei Wenzhou Xiang Tao Li Shengjie Lin Jiayi Wu Zishuo Chen Houbo Wu Chuanmao Li Hualian Wu |
| author_facet | Na Wang Haiwei Pei Wenzhou Xiang Tao Li Shengjie Lin Jiayi Wu Zishuo Chen Houbo Wu Chuanmao Li Hualian Wu |
| author_sort | Na Wang |
| collection | DOAJ |
| description | In order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water successively. Measuring the total phenol content (TPC), total carotenoid content (TCC), and antioxidant activity of the extracts, indicating TPC and TCC, played an important role in determining the antioxidant activity of the microalgae. A weighted scoring system was used to evaluate the antioxidant activity, and the scores of microalgal samples from two extraction methods were calculated using the same system. Among the investigated microalgae, <i>Euglena gracilis</i> SCSIO-46781 had the highest antioxidant score, contributing to high TPC and TCC, followed by <i>Arthrospira platensis</i> SCSIO-44012, <i>Nannochloropsis</i> sp. SCSIO-45224, <i>Phaeodactylum tricornutum</i> SCSIO-45120, and <i>Nannochloropsis</i> sp. SCSIO-45006, respectively. Additionally, the above-mentioned five strains are currently being applied in commercial production, indicating this system could be effective not only for screening microalgal antioxidants, but also for screening microalgal species/strains with strong adaptation to environmental stress, which is a critical trait for their commercial cultivation. |
| first_indexed | 2024-03-11T01:05:10Z |
| format | Article |
| id | doaj.art-e8c40607ff2a430eaf8a6199179f51ca |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T01:05:10Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-e8c40607ff2a430eaf8a6199179f51ca2023-11-18T19:19:22ZengMDPI AGFoods2304-81582023-07-011214265210.3390/foods12142652Rapid Screening of Microalgae as Potential Sources of Natural AntioxidantsNa Wang0Haiwei Pei1Wenzhou Xiang2Tao Li3Shengjie Lin4Jiayi Wu5Zishuo Chen6Houbo Wu7Chuanmao Li8Hualian Wu9CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaGuangzhou Keneng Cosmetic Scientific Research Co., Ltd., Guanghzou 510800, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaGuangzhou Keneng Cosmetic Scientific Research Co., Ltd., Guanghzou 510800, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaIn order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water successively. Measuring the total phenol content (TPC), total carotenoid content (TCC), and antioxidant activity of the extracts, indicating TPC and TCC, played an important role in determining the antioxidant activity of the microalgae. A weighted scoring system was used to evaluate the antioxidant activity, and the scores of microalgal samples from two extraction methods were calculated using the same system. Among the investigated microalgae, <i>Euglena gracilis</i> SCSIO-46781 had the highest antioxidant score, contributing to high TPC and TCC, followed by <i>Arthrospira platensis</i> SCSIO-44012, <i>Nannochloropsis</i> sp. SCSIO-45224, <i>Phaeodactylum tricornutum</i> SCSIO-45120, and <i>Nannochloropsis</i> sp. SCSIO-45006, respectively. Additionally, the above-mentioned five strains are currently being applied in commercial production, indicating this system could be effective not only for screening microalgal antioxidants, but also for screening microalgal species/strains with strong adaptation to environmental stress, which is a critical trait for their commercial cultivation.https://www.mdpi.com/2304-8158/12/14/2652microalgaeantioxidant activitycarotenoid contentphenolic contentweighted scoring system |
| spellingShingle | Na Wang Haiwei Pei Wenzhou Xiang Tao Li Shengjie Lin Jiayi Wu Zishuo Chen Houbo Wu Chuanmao Li Hualian Wu Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants Foods microalgae antioxidant activity carotenoid content phenolic content weighted scoring system |
| title | Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants |
| title_full | Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants |
| title_fullStr | Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants |
| title_full_unstemmed | Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants |
| title_short | Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants |
| title_sort | rapid screening of microalgae as potential sources of natural antioxidants |
| topic | microalgae antioxidant activity carotenoid content phenolic content weighted scoring system |
| url | https://www.mdpi.com/2304-8158/12/14/2652 |
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