<i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
Macrophages, cells effective in sensing, internalizing and killing <i>Candida albicans</i>, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/5/392 |
| _version_ | 1797533782736109568 |
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| author | Mónica Cicuéndez Laura Casarrubios María José Feito Iratxe Madarieta Nerea Garcia-Urkia Olatz Murua Beatriz Olalde Nerea Briz Rosalía Diez-Orejas María Teresa Portolés |
| author_facet | Mónica Cicuéndez Laura Casarrubios María José Feito Iratxe Madarieta Nerea Garcia-Urkia Olatz Murua Beatriz Olalde Nerea Briz Rosalía Diez-Orejas María Teresa Portolés |
| author_sort | Mónica Cicuéndez |
| collection | DOAJ |
| description | Macrophages, cells effective in sensing, internalizing and killing <i>Candida albicans</i>, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/<i>Candida albicans</i> interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario. |
| first_indexed | 2024-03-10T11:20:31Z |
| format | Article |
| id | doaj.art-e8c6544bc5a3432d99a80482df2dea43 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T11:20:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-e8c6544bc5a3432d99a80482df2dea432023-11-21T20:05:52ZengMDPI AGJournal of Fungi2309-608X2021-05-017539210.3390/jof7050392<i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose MatricesMónica Cicuéndez0Laura Casarrubios1María José Feito2Iratxe Madarieta3Nerea Garcia-Urkia4Olatz Murua5Beatriz Olalde6Nerea Briz7Rosalía Diez-Orejas8María Teresa Portolés9Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Universidad Complutense de Madrid, 28040 Madrid, SpainTECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, SpainTECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, SpainTECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, SpainTECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, SpainTECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, SpainDepartamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Universidad Complutense de Madrid, 28040 Madrid, SpainMacrophages, cells effective in sensing, internalizing and killing <i>Candida albicans</i>, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/<i>Candida albicans</i> interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.https://www.mdpi.com/2309-608X/7/5/392<i>Candida albicans</i>macrophageextracellular matrixdecellularizationimmunocompetencephagocytosis |
| spellingShingle | Mónica Cicuéndez Laura Casarrubios María José Feito Iratxe Madarieta Nerea Garcia-Urkia Olatz Murua Beatriz Olalde Nerea Briz Rosalía Diez-Orejas María Teresa Portolés <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices Journal of Fungi <i>Candida albicans</i> macrophage extracellular matrix decellularization immunocompetence phagocytosis |
| title | <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
| title_full | <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
| title_fullStr | <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
| title_full_unstemmed | <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
| title_short | <i>Candida albicans</i>/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
| title_sort | i candida albicans i macrophage biointerface on human and porcine decellularized adipose matrices |
| topic | <i>Candida albicans</i> macrophage extracellular matrix decellularization immunocompetence phagocytosis |
| url | https://www.mdpi.com/2309-608X/7/5/392 |
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