The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery
Obesity and high-fat diet (HF) are prevalent causes of oxidative stress (OS). Duodenal-jejunal omega switch (DJOS) is a bariatric procedure used for body mass reduction, extensively tested in animal models. We studied the long-term impact of bariatric surgery and an HF diet on the oxidative stress m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/2/183 |
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| author | Bronisława Skrzep-Poloczek Jakub Poloczek Elżbieta Chełmecka Agnieszka Dulska Ewa Romuk Maciej Idzik Wojciech Kazura Katarzyna Nabrdalik Janusz Gumprecht Jerzy Jochem Dominika Marta Stygar |
| author_facet | Bronisława Skrzep-Poloczek Jakub Poloczek Elżbieta Chełmecka Agnieszka Dulska Ewa Romuk Maciej Idzik Wojciech Kazura Katarzyna Nabrdalik Janusz Gumprecht Jerzy Jochem Dominika Marta Stygar |
| author_sort | Bronisława Skrzep-Poloczek |
| collection | DOAJ |
| description | Obesity and high-fat diet (HF) are prevalent causes of oxidative stress (OS). Duodenal-jejunal omega switch (DJOS) is a bariatric procedure used for body mass reduction, extensively tested in animal models. We studied the long-term impact of bariatric surgery and an HF diet on the oxidative stress markers in erythrocytes and heart muscles of rats. We analyzed superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) activity and malondialdehyde (MDA) concentration in DJOS or SHAM (control) operated rats fed with different dietary protocols (control diet (CD) and high-fat diet (HF)), before and after the surgery (CD/CD, HF/HF, CD/HF, and HF/CD). We observed higher erythrocytes CAT, GST and GPx activity in DJOS-operated (vs. SHAM) rats fed with an HF/HF diet. For DJOS-operated rats, erythrocytes CAT and GPx activity and MDA concentration were significantly lower in CD/CD group. We observed increased heart muscle GR activity in SHAM-operated rats (vs. DJOS bariatric surgery) fed with an HF/HF diet. Change from HF to CD diet increased heart muscle GPx activity after DJOS bariatric surgery. Heart muscle SOD activity was lower in HF/HF and CD/CD groups after DJOS bariatric surgery (vs. SHAM). DJOS surgery significantly reduced heart muscle MDA concentration in HF/HF and HF/CD groups (vs. SHAM). We conclude that the selected dietary patterns had a stronger impact on oxidative stress markers in erythrocytes and heart muscle than DJOS bariatric surgery. |
| first_indexed | 2024-03-12T05:40:02Z |
| format | Article |
| id | doaj.art-e8c8fadf145448a8bd0efe7705d83112 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T05:40:02Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-e8c8fadf145448a8bd0efe7705d831122023-09-03T06:11:44ZengMDPI AGAntioxidants2076-39212020-02-019218310.3390/antiox9020183antiox9020183The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric SurgeryBronisława Skrzep-Poloczek0Jakub Poloczek1Elżbieta Chełmecka2Agnieszka Dulska3Ewa Romuk4Maciej Idzik5Wojciech Kazura6Katarzyna Nabrdalik7Janusz Gumprecht8Jerzy Jochem9Dominika Marta Stygar10Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandDepartment of Rehabilitation, 3rd Specialist Hospital in Rybnik, 44-200 Rybnik, PolandDepartment of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec Medical University of Silesia, 40-751 Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandDepartment of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandIndependent Public Health Care, Opole Cancer Center prof. Tadeusz Koszarowski, 45-061 Opole, PolandDepartment of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandDepartment of Internal Medicine, Diabetology and Nephrology in Zabrze, Medical University of Silesia, 45-061 Katowice, PolandDepartment of Internal Medicine, Diabetology and Nephrology in Zabrze, Medical University of Silesia, 45-061 Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, PolandObesity and high-fat diet (HF) are prevalent causes of oxidative stress (OS). Duodenal-jejunal omega switch (DJOS) is a bariatric procedure used for body mass reduction, extensively tested in animal models. We studied the long-term impact of bariatric surgery and an HF diet on the oxidative stress markers in erythrocytes and heart muscles of rats. We analyzed superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) activity and malondialdehyde (MDA) concentration in DJOS or SHAM (control) operated rats fed with different dietary protocols (control diet (CD) and high-fat diet (HF)), before and after the surgery (CD/CD, HF/HF, CD/HF, and HF/CD). We observed higher erythrocytes CAT, GST and GPx activity in DJOS-operated (vs. SHAM) rats fed with an HF/HF diet. For DJOS-operated rats, erythrocytes CAT and GPx activity and MDA concentration were significantly lower in CD/CD group. We observed increased heart muscle GR activity in SHAM-operated rats (vs. DJOS bariatric surgery) fed with an HF/HF diet. Change from HF to CD diet increased heart muscle GPx activity after DJOS bariatric surgery. Heart muscle SOD activity was lower in HF/HF and CD/CD groups after DJOS bariatric surgery (vs. SHAM). DJOS surgery significantly reduced heart muscle MDA concentration in HF/HF and HF/CD groups (vs. SHAM). We conclude that the selected dietary patterns had a stronger impact on oxidative stress markers in erythrocytes and heart muscle than DJOS bariatric surgery.https://www.mdpi.com/2076-3921/9/2/183high-fat diethf dietbariatric surgeryoxidative stressdjosantioxidants |
| spellingShingle | Bronisława Skrzep-Poloczek Jakub Poloczek Elżbieta Chełmecka Agnieszka Dulska Ewa Romuk Maciej Idzik Wojciech Kazura Katarzyna Nabrdalik Janusz Gumprecht Jerzy Jochem Dominika Marta Stygar The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery Antioxidants high-fat diet hf diet bariatric surgery oxidative stress djos antioxidants |
| title | The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery |
| title_full | The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery |
| title_fullStr | The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery |
| title_full_unstemmed | The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery |
| title_short | The Oxidative Stress Markers in the Erythrocytes and Heart Muscle of Obese Rats: Relate to a High-Fat Diet but Not to DJOS Bariatric Surgery |
| title_sort | oxidative stress markers in the erythrocytes and heart muscle of obese rats relate to a high fat diet but not to djos bariatric surgery |
| topic | high-fat diet hf diet bariatric surgery oxidative stress djos antioxidants |
| url | https://www.mdpi.com/2076-3921/9/2/183 |
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