Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya
Background: Non-Tuberculous Mycobacteria (NTM) transmission to humans occurs through inhalation of dust particles or vaporized water containing NTM leading to pulmonary manifestations. NTM infections are often misdiagnosed for tuberculosis (TB) due to their similar clinical and radiological manifest...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=60;epage=69;aulast=Mwangi |
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| author | Zakayo Maingi Mwangi Nellie Njambi Mukiri Frank Gekara Onyambu Bulimo Dimbuson Wallace |
| author_facet | Zakayo Maingi Mwangi Nellie Njambi Mukiri Frank Gekara Onyambu Bulimo Dimbuson Wallace |
| author_sort | Zakayo Maingi Mwangi |
| collection | DOAJ |
| description | Background: Non-Tuberculous Mycobacteria (NTM) transmission to humans occurs through inhalation of dust particles or vaporized water containing NTM leading to pulmonary manifestations. NTM infections are often misdiagnosed for tuberculosis (TB) due to their similar clinical and radiological manifestations. Aims and Objectives: We, therefore, performed a species-level identification of NTM in symptomatic TB negative patients through sequencing of the hsp65 gene. Materials and Methods: We conducted a cross-sectional study at the National Tuberculosis Reference Laboratory in the period between January to November 2020. One hundred and sixty-six mycobacterial culture-positive samples that tested negative for TB using capilia underwent Polymerase Chain Reaction targeting the hsp65 gene. Isolates showing a band with gel electrophoresis at 441 bp position were sequenced using Sanger technology. Geneious software was used to analyze the obtained sequences, and the National Center for Biotechnology Information gene database identified NTM species for each isolate. A phylogenetic tree was constructed from the DNA sequences and evolutionary distances computed using the general time-reversible method. Pearson chi-square was used to determine the association between NTM infection and participants' characteristics. Results: Our study identified 43 different NTM species. The dominant NTM belonged to Mycobacterium avium complex 37 (31%). Slow-growing NTM were the majority at 86 (71%) while rapid-growing NTM were 36 (29%). A significant association (P<0.05) was observed for regions and age, while patient type had a weak likelihood of NTM infection. Conclusion: Our study characterized the diversity of NTM in Kenya for the first time and showed that species belonging to M. Avium Complex are the most prevalent in the country. |
| first_indexed | 2024-04-12T13:23:26Z |
| format | Article |
| id | doaj.art-e8ca22e41f3f4cf4a27805cb3dd2f5e3 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-04-12T13:23:26Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-e8ca22e41f3f4cf4a27805cb3dd2f5e32022-12-22T03:31:25ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2022-01-01111606910.4103/ijmy.ijmy_224_21Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in KenyaZakayo Maingi MwangiNellie Njambi MukiriFrank Gekara OnyambuBulimo Dimbuson WallaceBackground: Non-Tuberculous Mycobacteria (NTM) transmission to humans occurs through inhalation of dust particles or vaporized water containing NTM leading to pulmonary manifestations. NTM infections are often misdiagnosed for tuberculosis (TB) due to their similar clinical and radiological manifestations. Aims and Objectives: We, therefore, performed a species-level identification of NTM in symptomatic TB negative patients through sequencing of the hsp65 gene. Materials and Methods: We conducted a cross-sectional study at the National Tuberculosis Reference Laboratory in the period between January to November 2020. One hundred and sixty-six mycobacterial culture-positive samples that tested negative for TB using capilia underwent Polymerase Chain Reaction targeting the hsp65 gene. Isolates showing a band with gel electrophoresis at 441 bp position were sequenced using Sanger technology. Geneious software was used to analyze the obtained sequences, and the National Center for Biotechnology Information gene database identified NTM species for each isolate. A phylogenetic tree was constructed from the DNA sequences and evolutionary distances computed using the general time-reversible method. Pearson chi-square was used to determine the association between NTM infection and participants' characteristics. Results: Our study identified 43 different NTM species. The dominant NTM belonged to Mycobacterium avium complex 37 (31%). Slow-growing NTM were the majority at 86 (71%) while rapid-growing NTM were 36 (29%). A significant association (P<0.05) was observed for regions and age, while patient type had a weak likelihood of NTM infection. Conclusion: Our study characterized the diversity of NTM in Kenya for the first time and showed that species belonging to M. Avium Complex are the most prevalent in the country.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=60;epage=69;aulast=Mwangihsp65nontuberculous mycobacteria genetic diversitynontuberculous mycobacteriarapid-growing nontuberculous mycobacteriaslow-growing nontuberculous mycobacteria |
| spellingShingle | Zakayo Maingi Mwangi Nellie Njambi Mukiri Frank Gekara Onyambu Bulimo Dimbuson Wallace Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya International Journal of Mycobacteriology hsp65 nontuberculous mycobacteria genetic diversity nontuberculous mycobacteria rapid-growing nontuberculous mycobacteria slow-growing nontuberculous mycobacteria |
| title | Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya |
| title_full | Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya |
| title_fullStr | Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya |
| title_full_unstemmed | Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya |
| title_short | Genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in Kenya |
| title_sort | genetic diversity of nontuberculous mycobacteria among symptomatic tuberculosis negative patients in kenya |
| topic | hsp65 nontuberculous mycobacteria genetic diversity nontuberculous mycobacteria rapid-growing nontuberculous mycobacteria slow-growing nontuberculous mycobacteria |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=1;spage=60;epage=69;aulast=Mwangi |
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