Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas
Background. Transplantation of beta cells by pancreas or islet transplantation is the treatment of choice for a selected group of patients suffering from type 1 diabetes mellitus. Pancreata are frequently not accepted for transplantation, because of the relatively high vulnerability of these organs...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2018-10-01
|
| Series: | Transplantation Direct |
| Online Access: | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000829 |
| _version_ | 1811334657594097664 |
|---|---|
| author | Marjolein Leemkuil, MD Grietje Lier, MD Marten A. Engelse, PhD Rutger J. Ploeg, PhD Eelco J. P. de Koning, PhD Nils A. ‘t Hart, PhD Christina Krikke, MD Henri G. D. Leuvenink, PhD |
| author_facet | Marjolein Leemkuil, MD Grietje Lier, MD Marten A. Engelse, PhD Rutger J. Ploeg, PhD Eelco J. P. de Koning, PhD Nils A. ‘t Hart, PhD Christina Krikke, MD Henri G. D. Leuvenink, PhD |
| author_sort | Marjolein Leemkuil, MD |
| collection | DOAJ |
| description | Background. Transplantation of beta cells by pancreas or islet transplantation is the treatment of choice for a selected group of patients suffering from type 1 diabetes mellitus. Pancreata are frequently not accepted for transplantation, because of the relatively high vulnerability of these organs to ischemic injury. In this study, we evaluated the effects of hypothermic machine perfusion (HMP) on the quality of human pancreas grafts.
Methods. Five pancreata derived from donation after circulatory death (DCD) and 5 from donation after brain death (DBD) donors were preserved by oxygenated HMP. Hypothermic machine perfusion was performed for 6 hours at 25 mm Hg by separate perfusion of the mesenteric superior artery and the splenic artery. Results were compared with those of 10 pancreata preserved by static cold storage.
Results. During HMP, homogeneous perfusion of the pancreas could be achieved. Adenosine 5′-triphosphate concentration increased 6,8-fold in DCD and 2,6-fold in DBD pancreata. No signs of cellular injury, edema or formation of reactive oxygen species were observed. Islets of Langerhans with good viability and in vitro function could be isolated after HMP.
Conclusions. Oxygenated HMP is a feasible and safe preservation method for the human pancreas that increases tissue viability. |
| first_indexed | 2024-04-13T17:12:17Z |
| format | Article |
| id | doaj.art-e8cb835bdc2b41cb883976ee92e06ccd |
| institution | Directory Open Access Journal |
| issn | 2373-8731 |
| language | English |
| last_indexed | 2024-04-13T17:12:17Z |
| publishDate | 2018-10-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Transplantation Direct |
| spelling | doaj.art-e8cb835bdc2b41cb883976ee92e06ccd2022-12-22T02:38:15ZengWolters KluwerTransplantation Direct2373-87312018-10-01410e38810.1097/TXD.0000000000000829201810000-0008Hypothermic Oxygenated Machine Perfusion of the Human Donor PancreasMarjolein Leemkuil, MD0Grietje Lier, MD1Marten A. Engelse, PhD2Rutger J. Ploeg, PhD3Eelco J. P. de Koning, PhD4Nils A. ‘t Hart, PhD5Christina Krikke, MD6Henri G. D. Leuvenink, PhD71 Departments of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.1 Departments of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.2 Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands.3 Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom.2 Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands.5 Department of Pathology, University Medical Center Groningen, Groningen, The Netherlands.1 Departments of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.1 Departments of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.Background. Transplantation of beta cells by pancreas or islet transplantation is the treatment of choice for a selected group of patients suffering from type 1 diabetes mellitus. Pancreata are frequently not accepted for transplantation, because of the relatively high vulnerability of these organs to ischemic injury. In this study, we evaluated the effects of hypothermic machine perfusion (HMP) on the quality of human pancreas grafts. Methods. Five pancreata derived from donation after circulatory death (DCD) and 5 from donation after brain death (DBD) donors were preserved by oxygenated HMP. Hypothermic machine perfusion was performed for 6 hours at 25 mm Hg by separate perfusion of the mesenteric superior artery and the splenic artery. Results were compared with those of 10 pancreata preserved by static cold storage. Results. During HMP, homogeneous perfusion of the pancreas could be achieved. Adenosine 5′-triphosphate concentration increased 6,8-fold in DCD and 2,6-fold in DBD pancreata. No signs of cellular injury, edema or formation of reactive oxygen species were observed. Islets of Langerhans with good viability and in vitro function could be isolated after HMP. Conclusions. Oxygenated HMP is a feasible and safe preservation method for the human pancreas that increases tissue viability.http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000829 |
| spellingShingle | Marjolein Leemkuil, MD Grietje Lier, MD Marten A. Engelse, PhD Rutger J. Ploeg, PhD Eelco J. P. de Koning, PhD Nils A. ‘t Hart, PhD Christina Krikke, MD Henri G. D. Leuvenink, PhD Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas Transplantation Direct |
| title | Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas |
| title_full | Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas |
| title_fullStr | Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas |
| title_full_unstemmed | Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas |
| title_short | Hypothermic Oxygenated Machine Perfusion of the Human Donor Pancreas |
| title_sort | hypothermic oxygenated machine perfusion of the human donor pancreas |
| url | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000829 |
| work_keys_str_mv | AT marjoleinleemkuilmd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT grietjeliermd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT martenaengelsephd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT rutgerjploegphd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT eelcojpdekoningphd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT nilsathartphd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT christinakrikkemd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas AT henrigdleuveninkphd hypothermicoxygenatedmachineperfusionofthehumandonorpancreas |