Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing.
The oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral mic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4489859?pdf=render |
| _version_ | 1818130917823086592 |
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| author | Changin Oh Kunkyu Lee Yeotaek Cheong Sang-Won Lee Seung-Yong Park Chang-Seon Song In-Soo Choi Joong-Bok Lee |
| author_facet | Changin Oh Kunkyu Lee Yeotaek Cheong Sang-Won Lee Seung-Yong Park Chang-Seon Song In-Soo Choi Joong-Bok Lee |
| author_sort | Changin Oh |
| collection | DOAJ |
| description | The oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral microbiome, which may include human pathogenic bacteria, and its relationship with that of their owners are unclear. In this study, 16S rDNA pyrosequencing was used to compare the oral microbiomes of 10 dogs and their owners and to identify zoonotic pathogens. Pyrosequencing revealed 246 operational taxonomic units in the 10 samples, representing 57 genera from eight bacterial phyla. Firmicutes (57.6%), Proteobacteria (21.6%), Bacteroidetes (9.8%), Actinobacteria (7.1%), and Fusobacteria (3.9%) were the predominant phyla in the human oral samples, whereas Proteobacteria (25.7%), Actinobacteria (21%), Bacteroidetes (19.7%), Firmicutes (19.3%), and Fusobacteria (12.3%) were predominant in the canine oral samples. The predominant genera in the human samples were Streptococcus (43.9%), Neisseria (10.3%), Haemophilus (9.6%), Prevotella (8.4%), and Veillonella (8.1%), whereas the predominant genera in the canine samples were Actinomyces (17.2%), Unknown (16.8), Porphyromonas (14.8), Fusobacterium (11.8), and Neisseria (7.2%). The oral microbiomes of dogs and their owners were appreciably different, and similarity in the microbiomes of canines and their owners was not correlated with residing in the same household. Oral-to-oral transfer of Neisseria shayeganii, Porphyromonas canigingivalis, Tannerella forsythia, and Streptococcus minor from dogs to humans was suspected. The finding of potentially zoonotic and periodontopathic bacteria in the canine oral microbiome may be a public health concern. |
| first_indexed | 2024-12-11T08:12:40Z |
| format | Article |
| id | doaj.art-e8cec815c82042ca9488252ce4ba7374 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:12:40Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e8cec815c82042ca9488252ce4ba73742022-12-22T01:14:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013146810.1371/journal.pone.0131468Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing.Changin OhKunkyu LeeYeotaek CheongSang-Won LeeSeung-Yong ParkChang-Seon SongIn-Soo ChoiJoong-Bok LeeThe oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral microbiome, which may include human pathogenic bacteria, and its relationship with that of their owners are unclear. In this study, 16S rDNA pyrosequencing was used to compare the oral microbiomes of 10 dogs and their owners and to identify zoonotic pathogens. Pyrosequencing revealed 246 operational taxonomic units in the 10 samples, representing 57 genera from eight bacterial phyla. Firmicutes (57.6%), Proteobacteria (21.6%), Bacteroidetes (9.8%), Actinobacteria (7.1%), and Fusobacteria (3.9%) were the predominant phyla in the human oral samples, whereas Proteobacteria (25.7%), Actinobacteria (21%), Bacteroidetes (19.7%), Firmicutes (19.3%), and Fusobacteria (12.3%) were predominant in the canine oral samples. The predominant genera in the human samples were Streptococcus (43.9%), Neisseria (10.3%), Haemophilus (9.6%), Prevotella (8.4%), and Veillonella (8.1%), whereas the predominant genera in the canine samples were Actinomyces (17.2%), Unknown (16.8), Porphyromonas (14.8), Fusobacterium (11.8), and Neisseria (7.2%). The oral microbiomes of dogs and their owners were appreciably different, and similarity in the microbiomes of canines and their owners was not correlated with residing in the same household. Oral-to-oral transfer of Neisseria shayeganii, Porphyromonas canigingivalis, Tannerella forsythia, and Streptococcus minor from dogs to humans was suspected. The finding of potentially zoonotic and periodontopathic bacteria in the canine oral microbiome may be a public health concern.http://europepmc.org/articles/PMC4489859?pdf=render |
| spellingShingle | Changin Oh Kunkyu Lee Yeotaek Cheong Sang-Won Lee Seung-Yong Park Chang-Seon Song In-Soo Choi Joong-Bok Lee Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. PLoS ONE |
| title | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. |
| title_full | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. |
| title_fullStr | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. |
| title_full_unstemmed | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. |
| title_short | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing. |
| title_sort | comparison of the oral microbiomes of canines and their owners using next generation sequencing |
| url | http://europepmc.org/articles/PMC4489859?pdf=render |
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