Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs
<p><strong>Background and Objective:</strong> Berberine has anti-inflammatory properties through pathways that are also involved in asthma. Therefore, it is postulated that it will be effective in reducing airway inflammation in the allergic asthmatic model. The objective of the cu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Discover STM Publishing Ltd
2023-06-01
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| Series: | BioMedica |
| Online Access: | https://biomedicapk.com/10.24911/BioMedica/5-886 |
| _version_ | 1797630496914538496 |
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| author | Syeda Tahira Zaidi Mahwash Malik Javeria Sarfraz Sadia Chiragh |
| author_facet | Syeda Tahira Zaidi Mahwash Malik Javeria Sarfraz Sadia Chiragh |
| author_sort | Syeda Tahira Zaidi |
| collection | DOAJ |
| description | <p><strong>Background and Objective:</strong> Berberine has anti-inflammatory properties through pathways that are also involved in asthma. Therefore, it is postulated that it will be effective in reducing airway inflammation in the allergic asthmatic model. The objective of the current study was to observe the effects of berberine on the histopathology of lungs in sensitized Guinea pigs and compare the changes with that of the standard drug, dexamethasone.</p>
<p><strong>Methods:</strong> This experimental study was conducted at the Postgraduate Medical Institute, Lahore, Pakistan, from February 2016 to April 2016. Twenty-four healthy Guinea pigs were divided randomly into four groups: normal control, Ovalbumin (OVA ) group, OVA + berberine group, and OVA + dexa group. The last three groups were sensitized on days 0 and 14 and challenged on days 25, 26, and 27 with OVA. Berberine and dexamethasone were administered intraperitoneally to the respective groups before each challenge. The animals were then sacrificed on day 28 under anesthesia, lungs were dissected, and tissue samples were assessed microscopically for morphology and status of inflammation in each group. Data were analyzed by applying the Kruskal-Wallis analysis of variance test followed by the Mann Whitney U test using SPSS 20.</p>
<p><strong>Results:</strong> The comparison of histopathological changes revealed that the infiltration of inflammatory cells in the lung airways was significantly higher (p-value ≤0.01) in the OVA group as compared to the normal control group. Both OVA + berberine and OVA + dexa groups showed a reduction in total lung inflammation (p-value ≤0.01 vs. OVA group) equally. The effect of berberine on epithelial changes was nonsignificant (p-value 0.206 vs. OVA group), while dexamethasone showed significant improvement as compared to the OVA group (p-value 0.007). However, the difference between berberine and dexamethasone treatment was nonsignificant (p-value ≤0.05).</p>
<p><strong>Conclusion: </strong>It was concluded that berberine improved the histopathological score of lung inflammation in sensitized Guinea pigs, equivalent to that of dexamethasone, with lesser effects on epithelial changes.</p> |
| first_indexed | 2024-03-11T11:08:57Z |
| format | Article |
| id | doaj.art-e8cfd30983044aa68cb9adc350301711 |
| institution | Directory Open Access Journal |
| issn | 2710-3471 |
| language | English |
| last_indexed | 2024-03-11T11:08:57Z |
| publishDate | 2023-06-01 |
| publisher | Discover STM Publishing Ltd |
| record_format | Article |
| series | BioMedica |
| spelling | doaj.art-e8cfd30983044aa68cb9adc3503017112023-11-12T11:29:50ZengDiscover STM Publishing LtdBioMedica2710-34712023-06-01392546010.24911/BioMedica/5-886Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea PigsSyeda Tahira Zaidi0Mahwash Malik1Javeria Sarfraz2Sadia Chiragh3Senior Demonstrator, Department of Pharmacology, Al-Aleem Medical College, Lahore, Pakistan.Associate Professor, Department of Pharmacology, Central Park Medical College, Lahore, Pakistan.Assistant Professor, Department of Pharmacology, King Edward Medical University, Lahore, Pakistan.Professor, Department of Pharmacology, Al-Aleem Medical College, Lahore, Pakistan.<p><strong>Background and Objective:</strong> Berberine has anti-inflammatory properties through pathways that are also involved in asthma. Therefore, it is postulated that it will be effective in reducing airway inflammation in the allergic asthmatic model. The objective of the current study was to observe the effects of berberine on the histopathology of lungs in sensitized Guinea pigs and compare the changes with that of the standard drug, dexamethasone.</p> <p><strong>Methods:</strong> This experimental study was conducted at the Postgraduate Medical Institute, Lahore, Pakistan, from February 2016 to April 2016. Twenty-four healthy Guinea pigs were divided randomly into four groups: normal control, Ovalbumin (OVA ) group, OVA + berberine group, and OVA + dexa group. The last three groups were sensitized on days 0 and 14 and challenged on days 25, 26, and 27 with OVA. Berberine and dexamethasone were administered intraperitoneally to the respective groups before each challenge. The animals were then sacrificed on day 28 under anesthesia, lungs were dissected, and tissue samples were assessed microscopically for morphology and status of inflammation in each group. Data were analyzed by applying the Kruskal-Wallis analysis of variance test followed by the Mann Whitney U test using SPSS 20.</p> <p><strong>Results:</strong> The comparison of histopathological changes revealed that the infiltration of inflammatory cells in the lung airways was significantly higher (p-value ≤0.01) in the OVA group as compared to the normal control group. Both OVA + berberine and OVA + dexa groups showed a reduction in total lung inflammation (p-value ≤0.01 vs. OVA group) equally. The effect of berberine on epithelial changes was nonsignificant (p-value 0.206 vs. OVA group), while dexamethasone showed significant improvement as compared to the OVA group (p-value 0.007). However, the difference between berberine and dexamethasone treatment was nonsignificant (p-value ≤0.05).</p> <p><strong>Conclusion: </strong>It was concluded that berberine improved the histopathological score of lung inflammation in sensitized Guinea pigs, equivalent to that of dexamethasone, with lesser effects on epithelial changes.</p>https://biomedicapk.com/10.24911/BioMedica/5-886 |
| spellingShingle | Syeda Tahira Zaidi Mahwash Malik Javeria Sarfraz Sadia Chiragh Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs BioMedica |
| title | Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs |
| title_full | Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs |
| title_fullStr | Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs |
| title_full_unstemmed | Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs |
| title_short | Berberine Improves Histopathological Changes of Allergic Airway Inflammation in Guinea Pigs |
| title_sort | berberine improves histopathological changes of allergic airway inflammation in guinea pigs |
| url | https://biomedicapk.com/10.24911/BioMedica/5-886 |
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