Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery
Pre-eclampsia is a harmful and potentially lethal medical condition during pregnancy clinically diagnosed by hypertension and commonly accompanied by proteinuria and multiorgan affections. According to the time of diagnosis, it is differentiated between early-onset (EO-PE) and late-onset preeclampsi...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/11/1644 |
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| author | Luis M. Garcia-Puente Oscar Fraile-Martinez Cielo García-Montero Julia Bujan Juan A. De León-Luis Coral Bravo Patrocinio Rodríguez-Benitez Pilar Pintado Francisco Javier Ruiz-Labarta Melchor Álvarez-Mon Natalio García-Honduvilla María J. Cancelo Miguel A. Saez Miguel A. Ortega |
| author_facet | Luis M. Garcia-Puente Oscar Fraile-Martinez Cielo García-Montero Julia Bujan Juan A. De León-Luis Coral Bravo Patrocinio Rodríguez-Benitez Pilar Pintado Francisco Javier Ruiz-Labarta Melchor Álvarez-Mon Natalio García-Honduvilla María J. Cancelo Miguel A. Saez Miguel A. Ortega |
| author_sort | Luis M. Garcia-Puente |
| collection | DOAJ |
| description | Pre-eclampsia is a harmful and potentially lethal medical condition during pregnancy clinically diagnosed by hypertension and commonly accompanied by proteinuria and multiorgan affections. According to the time of diagnosis, it is differentiated between early-onset (EO-PE) and late-onset preeclampsia (LO-PE). Despite being less dangerous and presenting distinct pathophysiological signatures, LO-PE has a greater prevalence than EO-PE, both having significant consequences on the placenta. Previous works have evidenced that exacerbated inflammation in this organ might play a potential pathogenic role in the development of pre-eclampsia, and there is some preliminary evidence that the hyperactivation of inflammasomes can be related to the altered immunoinflammatory responses observed in the placentas of these patients. However, the precise role of inflammasomes in the placentas of women with LO-PE remains to be fully understood. In this work, we have studied the gene and protein expression of the main components related to the canonical and non-canonical pathways of the inflammasome NLRP3 (NLRP3, ASC, caspase 1, caspase 5, caspase 8, interleukin 1β, and interleukin 18) in the placental tissue of women with LO-PE. Our results show a marked increase in all these components in the placentas of women who have undergone LO-PE, suggesting that NLRP3 inflammasome plays a potentially pathophysiological role in the development of this entity. Future works should aim to evaluate possible translational approaches to this dysregulation in these patients. |
| first_indexed | 2024-03-09T16:59:05Z |
| format | Article |
| id | doaj.art-e8d03bc098e44a13ac32b66f6564ee33 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T16:59:05Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-e8d03bc098e44a13ac32b66f6564ee332023-11-24T14:32:05ZengMDPI AGBiomolecules2218-273X2023-11-011311164410.3390/biom13111644Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome MachineryLuis M. Garcia-Puente0Oscar Fraile-Martinez1Cielo García-Montero2Julia Bujan3Juan A. De León-Luis4Coral Bravo5Patrocinio Rodríguez-Benitez6Pilar Pintado7Francisco Javier Ruiz-Labarta8Melchor Álvarez-Mon9Natalio García-Honduvilla10María J. Cancelo11Miguel A. Saez12Miguel A. Ortega13Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Public and Maternal and Child Health, School of Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainDepartment of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, SpainPre-eclampsia is a harmful and potentially lethal medical condition during pregnancy clinically diagnosed by hypertension and commonly accompanied by proteinuria and multiorgan affections. According to the time of diagnosis, it is differentiated between early-onset (EO-PE) and late-onset preeclampsia (LO-PE). Despite being less dangerous and presenting distinct pathophysiological signatures, LO-PE has a greater prevalence than EO-PE, both having significant consequences on the placenta. Previous works have evidenced that exacerbated inflammation in this organ might play a potential pathogenic role in the development of pre-eclampsia, and there is some preliminary evidence that the hyperactivation of inflammasomes can be related to the altered immunoinflammatory responses observed in the placentas of these patients. However, the precise role of inflammasomes in the placentas of women with LO-PE remains to be fully understood. In this work, we have studied the gene and protein expression of the main components related to the canonical and non-canonical pathways of the inflammasome NLRP3 (NLRP3, ASC, caspase 1, caspase 5, caspase 8, interleukin 1β, and interleukin 18) in the placental tissue of women with LO-PE. Our results show a marked increase in all these components in the placentas of women who have undergone LO-PE, suggesting that NLRP3 inflammasome plays a potentially pathophysiological role in the development of this entity. Future works should aim to evaluate possible translational approaches to this dysregulation in these patients.https://www.mdpi.com/2218-273X/13/11/1644placentalate-onset pre-eclampsia (LO-PE)NLRP3 inflammasomecaspasesinterleukin 1βinterleukin 18 |
| spellingShingle | Luis M. Garcia-Puente Oscar Fraile-Martinez Cielo García-Montero Julia Bujan Juan A. De León-Luis Coral Bravo Patrocinio Rodríguez-Benitez Pilar Pintado Francisco Javier Ruiz-Labarta Melchor Álvarez-Mon Natalio García-Honduvilla María J. Cancelo Miguel A. Saez Miguel A. Ortega Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery Biomolecules placenta late-onset pre-eclampsia (LO-PE) NLRP3 inflammasome caspases interleukin 1β interleukin 18 |
| title | Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery |
| title_full | Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery |
| title_fullStr | Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery |
| title_full_unstemmed | Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery |
| title_short | Placentas from Women with Late-Onset Preeclampsia Exhibit Increased Expression of the NLRP3 Inflammasome Machinery |
| title_sort | placentas from women with late onset preeclampsia exhibit increased expression of the nlrp3 inflammasome machinery |
| topic | placenta late-onset pre-eclampsia (LO-PE) NLRP3 inflammasome caspases interleukin 1β interleukin 18 |
| url | https://www.mdpi.com/2218-273X/13/11/1644 |
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