Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species
Wenqi Luo,1,2 Yiming Wang,1,2 Feng Lin,1,2 Yixuan Liu,2,3 Rui Gu,1 Wanguo Liu,1 Chunsheng Xiao2,4 1Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China; 2Key Laboratory of Polymer Ecomaterials, Changchun Institute o...
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| Format: | Article |
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Dove Medical Press
2020-12-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/selenium-doped-carbon-quantum-dots-efficiently-ameliorate-secondary-sp-peer-reviewed-article-IJN |
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| author | Luo W Wang Y Lin F Liu Y Gu R Liu W Xiao C |
| author_facet | Luo W Wang Y Lin F Liu Y Gu R Liu W Xiao C |
| author_sort | Luo W |
| collection | DOAJ |
| description | Wenqi Luo,1,2 Yiming Wang,1,2 Feng Lin,1,2 Yixuan Liu,2,3 Rui Gu,1 Wanguo Liu,1 Chunsheng Xiao2,4 1Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China; 2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People’s Republic of China; 3School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, People’s Republic of China; 4Jilin Biomedical Polymers Engineering Laboratory, Changchun 130022, People’s Republic of ChinaCorrespondence: Wanguo Liu; Rui Gu Email liuwanguo6016@jlu.edu.cn; gurui@jlu.edu.cnBackground: The excess production of reactive oxygen species (ROS) after traumatic spinal cord injury (TSCI) has been identified as a leading cause of secondary injury, which can significantly exacerbate acute damage in the injured spinal cord. Thus, scavenging of ROS has emerged as an effective route to ameliorate secondary spinal cord injury.Purpose: Selenium-doped carbon quantum dots (Se-CQDs) with the ability to scavenge reactive oxygen species were prepared and used for efficiently ameliorating secondary injury in TSCI.Methods: Water-soluble Se-CQDs were easily synthesized via hydrothermal treatment of l-selenocystine. The chemical structure, size, and morphology of the Se-CQDs were characterized in detail. The biocompatibility and protective effects of the Se-CQDs against H2O2-induced oxidative damage were investigated in vitro. Moreover, the behavioral test, bladder function, histological observation, Western blot were used to investigate the neuroprotective effect of Se-CQDs in a rat model of contusion TSCI.Results: The obtained Se-CQDs exhibited good biocompatibility and remarkable protective effect against H2O2-induced oxidative damage in astrocytes and PC12 cells. Moreover, Se-CQDs displayed marked anti-inflammatory and anti-apoptotic activities, which thereby reduced the formation of glial scars and increased the survival of neurons with unscathed myelin sheaths in vivo. As a result, Se-CQDs were capable of largely improving locomotor function of rats with TSCI.Conclusion: This study suggests that Se-CQDs can be used as a promising therapeutic platform for ameliorating secondary injury in TSCI.Keywords: carbon quantum dots, spinal cord injury, reactive oxygen species, antioxidant, inflammation |
| first_indexed | 2024-12-20T21:11:32Z |
| format | Article |
| id | doaj.art-e8d327010fec4b89af24aa656d091e47 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-20T21:11:32Z |
| publishDate | 2020-12-01 |
| publisher | Dove Medical Press |
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| series | International Journal of Nanomedicine |
| spelling | doaj.art-e8d327010fec4b89af24aa656d091e472022-12-21T19:26:31ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-12-01Volume 15101131012560260Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen SpeciesLuo WWang YLin FLiu YGu RLiu WXiao CWenqi Luo,1,2 Yiming Wang,1,2 Feng Lin,1,2 Yixuan Liu,2,3 Rui Gu,1 Wanguo Liu,1 Chunsheng Xiao2,4 1Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China; 2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People’s Republic of China; 3School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, People’s Republic of China; 4Jilin Biomedical Polymers Engineering Laboratory, Changchun 130022, People’s Republic of ChinaCorrespondence: Wanguo Liu; Rui Gu Email liuwanguo6016@jlu.edu.cn; gurui@jlu.edu.cnBackground: The excess production of reactive oxygen species (ROS) after traumatic spinal cord injury (TSCI) has been identified as a leading cause of secondary injury, which can significantly exacerbate acute damage in the injured spinal cord. Thus, scavenging of ROS has emerged as an effective route to ameliorate secondary spinal cord injury.Purpose: Selenium-doped carbon quantum dots (Se-CQDs) with the ability to scavenge reactive oxygen species were prepared and used for efficiently ameliorating secondary injury in TSCI.Methods: Water-soluble Se-CQDs were easily synthesized via hydrothermal treatment of l-selenocystine. The chemical structure, size, and morphology of the Se-CQDs were characterized in detail. The biocompatibility and protective effects of the Se-CQDs against H2O2-induced oxidative damage were investigated in vitro. Moreover, the behavioral test, bladder function, histological observation, Western blot were used to investigate the neuroprotective effect of Se-CQDs in a rat model of contusion TSCI.Results: The obtained Se-CQDs exhibited good biocompatibility and remarkable protective effect against H2O2-induced oxidative damage in astrocytes and PC12 cells. Moreover, Se-CQDs displayed marked anti-inflammatory and anti-apoptotic activities, which thereby reduced the formation of glial scars and increased the survival of neurons with unscathed myelin sheaths in vivo. As a result, Se-CQDs were capable of largely improving locomotor function of rats with TSCI.Conclusion: This study suggests that Se-CQDs can be used as a promising therapeutic platform for ameliorating secondary injury in TSCI.Keywords: carbon quantum dots, spinal cord injury, reactive oxygen species, antioxidant, inflammationhttps://www.dovepress.com/selenium-doped-carbon-quantum-dots-efficiently-ameliorate-secondary-sp-peer-reviewed-article-IJNcarbon quantum dotsspinal cord injuryreactive oxygen speciesantioxidantinflammation |
| spellingShingle | Luo W Wang Y Lin F Liu Y Gu R Liu W Xiao C Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species International Journal of Nanomedicine carbon quantum dots spinal cord injury reactive oxygen species antioxidant inflammation |
| title | Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species |
| title_full | Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species |
| title_fullStr | Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species |
| title_full_unstemmed | Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species |
| title_short | Selenium-Doped Carbon Quantum Dots Efficiently Ameliorate Secondary Spinal Cord Injury via Scavenging Reactive Oxygen Species |
| title_sort | selenium doped carbon quantum dots efficiently ameliorate secondary spinal cord injury via scavenging reactive oxygen species |
| topic | carbon quantum dots spinal cord injury reactive oxygen species antioxidant inflammation |
| url | https://www.dovepress.com/selenium-doped-carbon-quantum-dots-efficiently-ameliorate-secondary-sp-peer-reviewed-article-IJN |
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