Plasma proteins facilitates placental transfer of polystyrene particles
Abstract Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this s...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-020-00676-5 |
| _version_ | 1798003454224891904 |
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| author | Michael M. Gruber Birgit Hirschmugl Natascha Berger Magdalena Holter Snježana Radulović Gerd Leitinger Laura Liesinger Andrea Berghold Eva Roblegg Ruth Birner-Gruenberger Vesna Bjelic-Radisic Christian Wadsack |
| author_facet | Michael M. Gruber Birgit Hirschmugl Natascha Berger Magdalena Holter Snježana Radulović Gerd Leitinger Laura Liesinger Andrea Berghold Eva Roblegg Ruth Birner-Gruenberger Vesna Bjelic-Radisic Christian Wadsack |
| author_sort | Michael M. Gruber |
| collection | DOAJ |
| description | Abstract Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications. |
| first_indexed | 2024-04-11T12:07:52Z |
| format | Article |
| id | doaj.art-e8d861398ea7426d81263d366b590ad2 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T12:07:52Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-e8d861398ea7426d81263d366b590ad22022-12-22T04:24:40ZengBMCJournal of Nanobiotechnology1477-31552020-09-0118111410.1186/s12951-020-00676-5Plasma proteins facilitates placental transfer of polystyrene particlesMichael M. Gruber0Birgit Hirschmugl1Natascha Berger2Magdalena Holter3Snježana Radulović4Gerd Leitinger5Laura Liesinger6Andrea Berghold7Eva Roblegg8Ruth Birner-Gruenberger9Vesna Bjelic-Radisic10Christian Wadsack11Department of Obstetrics and Gynecology, Medical University of GrazDepartment of Obstetrics and Gynecology, Medical University of GrazDepartment of Obstetrics and Gynecology, Medical University of GrazInstitute for Medical Informatics, Statistics and Documentation, Medical University of GrazDivision of Molecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of GrazResearch Unit Electron Microscopic Techniques, Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of GrazDiagnostic and Research Institute of Pathology, Diagnostic and Research Center for Molecular BioMedicine, Medical University of GrazInstitute for Medical Informatics, Statistics and Documentation, Medical University of GrazInstitute of Pharmaceutical Sciences, Department of Pharmaceutical Technology and Biopharmacy, University of GrazDiagnostic and Research Institute of Pathology, Diagnostic and Research Center for Molecular BioMedicine, Medical University of GrazDepartment of Obstetrics and Gynecology, Medical University of GrazDepartment of Obstetrics and Gynecology, Medical University of GrazAbstract Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications.http://link.springer.com/article/10.1186/s12951-020-00676-5NanoparticlePolystyreneBiocoronaDual ex vivo placental perfusionHuman placentaPlasma proteins |
| spellingShingle | Michael M. Gruber Birgit Hirschmugl Natascha Berger Magdalena Holter Snježana Radulović Gerd Leitinger Laura Liesinger Andrea Berghold Eva Roblegg Ruth Birner-Gruenberger Vesna Bjelic-Radisic Christian Wadsack Plasma proteins facilitates placental transfer of polystyrene particles Journal of Nanobiotechnology Nanoparticle Polystyrene Biocorona Dual ex vivo placental perfusion Human placenta Plasma proteins |
| title | Plasma proteins facilitates placental transfer of polystyrene particles |
| title_full | Plasma proteins facilitates placental transfer of polystyrene particles |
| title_fullStr | Plasma proteins facilitates placental transfer of polystyrene particles |
| title_full_unstemmed | Plasma proteins facilitates placental transfer of polystyrene particles |
| title_short | Plasma proteins facilitates placental transfer of polystyrene particles |
| title_sort | plasma proteins facilitates placental transfer of polystyrene particles |
| topic | Nanoparticle Polystyrene Biocorona Dual ex vivo placental perfusion Human placenta Plasma proteins |
| url | http://link.springer.com/article/10.1186/s12951-020-00676-5 |
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