Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples
Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the <i>Cryptosporidium</i> oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evalu...
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/209 |
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| author | Romy Razakandrainibe Célia Mérat Nathalie Kapel Marc Sautour Karine Guyot Gilles Gargala Jean-Jacques Ballet Patrice Le Pape French Cryptosporidiosis Network Frédéric Dalle Loïc Favennec |
| author_facet | Romy Razakandrainibe Célia Mérat Nathalie Kapel Marc Sautour Karine Guyot Gilles Gargala Jean-Jacques Ballet Patrice Le Pape French Cryptosporidiosis Network Frédéric Dalle Loïc Favennec |
| author_sort | Romy Razakandrainibe |
| collection | DOAJ |
| description | Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the <i>Cryptosporidium</i> oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting <i>Cryptosporidium</i> spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA <i>Cryptosporidium</i> kit, Savyon Diagnostics, Israel) for the detection of <i>Cryptosporidium</i> spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis. |
| first_indexed | 2024-03-09T04:12:06Z |
| format | Article |
| id | doaj.art-e8d90256d42e4cf38f2443bac1fe2ae9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:12:06Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-e8d90256d42e4cf38f2443bac1fe2ae92023-12-03T13:59:37ZengMDPI AGMicroorganisms2076-26072021-01-019220910.3390/microorganisms9020209Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool SamplesRomy Razakandrainibe0Célia Mérat1Nathalie Kapel2Marc Sautour3Karine Guyot4Gilles Gargala5Jean-Jacques Ballet6Patrice Le Pape7French Cryptosporidiosis NetworkFrédéric Dalle8Loïc Favennec9EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, FranceDepartment of Parasitology/Mycology, University Hospital of Nantes, 44093 Nantes, FranceDepartment of Parasitology/Mycology, Faculty of Pharmacy, Paris University, 75013 Paris, FranceDepartment of Parasitology/Mycology, University Hospital of Dijon, 21000 Dijon, FranceDepartment of Parasitology/Mycology, Lille Pasteur Institute, 59000 Lille, FranceEA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, FranceEA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, FranceDepartment of Parasitology/Mycology, University Hospital of Nantes, 44093 Nantes, FranceDepartment of Parasitology/Mycology, University Hospital of Dijon, 21000 Dijon, FranceEA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, FranceHuman cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the <i>Cryptosporidium</i> oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting <i>Cryptosporidium</i> spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA <i>Cryptosporidium</i> kit, Savyon Diagnostics, Israel) for the detection of <i>Cryptosporidium</i> spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.https://www.mdpi.com/2076-2607/9/2/209cryptosporidiosis<i>Cryptosporidium</i> spp.diagnosiscoproantigen ELISAlarge-scale epidemiology |
| spellingShingle | Romy Razakandrainibe Célia Mérat Nathalie Kapel Marc Sautour Karine Guyot Gilles Gargala Jean-Jacques Ballet Patrice Le Pape French Cryptosporidiosis Network Frédéric Dalle Loïc Favennec Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples Microorganisms cryptosporidiosis <i>Cryptosporidium</i> spp. diagnosis coproantigen ELISA large-scale epidemiology |
| title | Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples |
| title_full | Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples |
| title_fullStr | Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples |
| title_full_unstemmed | Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples |
| title_short | Multicenter Evaluation of an ELISA for the Detection of <em>Cryptosporidium </em>spp. Antigen in Clinical Human Stool Samples |
| title_sort | multicenter evaluation of an elisa for the detection of em cryptosporidium em spp antigen in clinical human stool samples |
| topic | cryptosporidiosis <i>Cryptosporidium</i> spp. diagnosis coproantigen ELISA large-scale epidemiology |
| url | https://www.mdpi.com/2076-2607/9/2/209 |
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