Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses
Increasing marine microplastics (MPs) pollution potentially threatens the stability of phytoplankton community structures in marine environments. MPs toxicities to microalgae are largely determined by particle size, while the size-dependent mechanisms are still not fully understood. In this study, t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322009125 |
| _version_ | 1828733242184826880 |
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| author | Bihan Zhang Xuexi Tang Qian Liu Luying Li Yirong Zhao Yan Zhao |
| author_facet | Bihan Zhang Xuexi Tang Qian Liu Luying Li Yirong Zhao Yan Zhao |
| author_sort | Bihan Zhang |
| collection | DOAJ |
| description | Increasing marine microplastics (MPs) pollution potentially threatens the stability of phytoplankton community structures in marine environments. MPs toxicities to microalgae are largely determined by particle size, while the size-dependent mechanisms are still not fully understood. In this study, two sizes (0.1 µm and 1 µm) of polystyrene (PS) MPs were used as experimental targets to systemically compare their different effecting mechanisms on the marine model diatom Thalassiosira pseudonana with respect to oxidative stress and photosynthesis. The results indicated the toxicity of 1 µm sized MPs was higher than 0.1 µm sized MPs regarding to population growth. In condition of similar microalgal population inhibition rates, we found more enhanced cellular oxidative stress and cell death happened in the 1 µm MPs treatments, which could be linked to higher zeta potential of 1 µm MPs and more severe cell surface damage; microalgal surface light shading and cellular pigments decline were more obvious in the 0.1 µm MPs treatment, which could be linked to high aggregation abilities of 0.1 µm MPs. Gene expressions supported the morphological and physiological findings on the transcriptional level. Environmental related MPs concentrations (5 μg L−1) also aroused gene expression changes of T. pseudonana while more changing genes were found under 0.1 µm MPs than 1 µm MPs. These results provide novel insights into the size-dependent mechanisms of MPs toxicity on marine microalgae, as well as their potential influence on the marine environment. |
| first_indexed | 2024-04-12T22:26:45Z |
| format | Article |
| id | doaj.art-e8dbcde211964c1e861eb39ccd8e734c |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T22:26:45Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-e8dbcde211964c1e861eb39ccd8e734c2022-12-22T03:14:08ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01244114072Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responsesBihan Zhang0Xuexi Tang1Qian Liu2Luying Li3Yirong Zhao4Yan Zhao5College of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, ChinaCollege of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Department of Marine Ecology, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; Correspondence to: Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong, PR China.Increasing marine microplastics (MPs) pollution potentially threatens the stability of phytoplankton community structures in marine environments. MPs toxicities to microalgae are largely determined by particle size, while the size-dependent mechanisms are still not fully understood. In this study, two sizes (0.1 µm and 1 µm) of polystyrene (PS) MPs were used as experimental targets to systemically compare their different effecting mechanisms on the marine model diatom Thalassiosira pseudonana with respect to oxidative stress and photosynthesis. The results indicated the toxicity of 1 µm sized MPs was higher than 0.1 µm sized MPs regarding to population growth. In condition of similar microalgal population inhibition rates, we found more enhanced cellular oxidative stress and cell death happened in the 1 µm MPs treatments, which could be linked to higher zeta potential of 1 µm MPs and more severe cell surface damage; microalgal surface light shading and cellular pigments decline were more obvious in the 0.1 µm MPs treatment, which could be linked to high aggregation abilities of 0.1 µm MPs. Gene expressions supported the morphological and physiological findings on the transcriptional level. Environmental related MPs concentrations (5 μg L−1) also aroused gene expression changes of T. pseudonana while more changing genes were found under 0.1 µm MPs than 1 µm MPs. These results provide novel insights into the size-dependent mechanisms of MPs toxicity on marine microalgae, as well as their potential influence on the marine environment.http://www.sciencedirect.com/science/article/pii/S0147651322009125MicroplasticsThalassiosira pseudonanaSizePhotosynthesisOxidative stressLight shading |
| spellingShingle | Bihan Zhang Xuexi Tang Qian Liu Luying Li Yirong Zhao Yan Zhao Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses Ecotoxicology and Environmental Safety Microplastics Thalassiosira pseudonana Size Photosynthesis Oxidative stress Light shading |
| title | Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| title_full | Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| title_fullStr | Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| title_full_unstemmed | Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| title_short | Different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| title_sort | different effecting mechanisms of two sized polystyrene microplastics on microalgal oxidative stress and photosynthetic responses |
| topic | Microplastics Thalassiosira pseudonana Size Photosynthesis Oxidative stress Light shading |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322009125 |
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