Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis
The aim of our study was to test anti-lithogenic activity of the new biomedical substance from pig kidneys (PK-BMS) on the experimentally induced nephrolithiasis. Materials and Methods: The experiments were performed with 30 male Wistar outbred rats weighting 220g-280g. The experimental animals we...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2017-03-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://ijbm.org/articles/IJBM_7(1)_OA4.pdf |
| _version_ | 1818807974356844544 |
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| author | Aleksandr Yu. Zharikov Igor P. Saldan Valery I. Kiselev Ganna V. Pavlyashik |
| author_facet | Aleksandr Yu. Zharikov Igor P. Saldan Valery I. Kiselev Ganna V. Pavlyashik |
| author_sort | Aleksandr Yu. Zharikov |
| collection | DOAJ |
| description | The aim of our study was to test anti-lithogenic activity of the new biomedical substance from pig kidneys (PK-BMS) on the experimentally induced nephrolithiasis.
Materials and Methods: The experiments were performed with 30 male Wistar outbred rats weighting 220g-280g. The experimental animals were divided into two groups. In the disease-control group (DCG), the animals received a 1% solution of EG for 6 weeks. The rats in therapy group (TG), beginning from the fourth week, in addition to the daily EG intake, received PK-BMS in the amount of 1.0 per rat. The production of this substance was carried out at Altaivitaminy ZAO (Biysk, Altai Krai, Russia) by the method of cool dehumidification. In daily urine, we determined the concentration of oxalate, phosphate and calcium ions, creatinine excretion every 3 or 4 days in both groups; the activity of LDH, GGT, and NAG was measured every 7 days in the injured kidney epithelium. For the study of free-radical oxidation activity and for morphological examination of animal kidneys, after 6 weeks of the experiment, 5 rats from each group were euthanized. The occurrence of calcium compounds was determined by von Kossa’s staining method.
Results: After a 6-week EG intake, we found the typical biochemical and morphological symptoms of experimental nephrolithiasis in rats of DCG: urine supersaturation with oxalate ions, significant increase in the activity of marker enzymes, activation of free-radical oxidation in the kidneys, and formation of calcium deposits in the kidneys. In TG, the new PK-BMS intake resulted in a significant alleviation of the experimental nephrolithiasi: significant decrease in the level of oxalate ions and activity of marker enzymes, reduction of free-radical oxidation in the kidneys, decrease in the number and size of calcium deposits in the area of renal papillae.
Conclusion: It was established that during the experimental nephrolithiasis, a three-week intake of the new PK-BMS is accompanied by a significant anti-lithogenic effect. |
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| id | doaj.art-e8ddbec153d342f3a99a0f242382d094 |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-12-18T19:34:11Z |
| publishDate | 2017-03-01 |
| publisher | International Medical Research and Development Corporation |
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| series | International Journal of Biomedicine |
| spelling | doaj.art-e8ddbec153d342f3a99a0f242382d0942022-12-21T20:55:39ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292017-03-0171414510.21103/Article7(1)_OA4Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of NephrolithiasisAleksandr Yu. Zharikov0Igor P. Saldan1Valery I. Kiselev2Ganna V. Pavlyashik3Altai State Medical UniversityAltai State Medical UniversityAltai State Medical UniversityAltai State Medical UniversityThe aim of our study was to test anti-lithogenic activity of the new biomedical substance from pig kidneys (PK-BMS) on the experimentally induced nephrolithiasis. Materials and Methods: The experiments were performed with 30 male Wistar outbred rats weighting 220g-280g. The experimental animals were divided into two groups. In the disease-control group (DCG), the animals received a 1% solution of EG for 6 weeks. The rats in therapy group (TG), beginning from the fourth week, in addition to the daily EG intake, received PK-BMS in the amount of 1.0 per rat. The production of this substance was carried out at Altaivitaminy ZAO (Biysk, Altai Krai, Russia) by the method of cool dehumidification. In daily urine, we determined the concentration of oxalate, phosphate and calcium ions, creatinine excretion every 3 or 4 days in both groups; the activity of LDH, GGT, and NAG was measured every 7 days in the injured kidney epithelium. For the study of free-radical oxidation activity and for morphological examination of animal kidneys, after 6 weeks of the experiment, 5 rats from each group were euthanized. The occurrence of calcium compounds was determined by von Kossa’s staining method. Results: After a 6-week EG intake, we found the typical biochemical and morphological symptoms of experimental nephrolithiasis in rats of DCG: urine supersaturation with oxalate ions, significant increase in the activity of marker enzymes, activation of free-radical oxidation in the kidneys, and formation of calcium deposits in the kidneys. In TG, the new PK-BMS intake resulted in a significant alleviation of the experimental nephrolithiasi: significant decrease in the level of oxalate ions and activity of marker enzymes, reduction of free-radical oxidation in the kidneys, decrease in the number and size of calcium deposits in the area of renal papillae. Conclusion: It was established that during the experimental nephrolithiasis, a three-week intake of the new PK-BMS is accompanied by a significant anti-lithogenic effect.http://ijbm.org/articles/IJBM_7(1)_OA4.pdfexperimental nephrolithiasisanti-lithogenic activitybiomedical substancepig kidneys |
| spellingShingle | Aleksandr Yu. Zharikov Igor P. Saldan Valery I. Kiselev Ganna V. Pavlyashik Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis International Journal of Biomedicine experimental nephrolithiasis anti-lithogenic activity biomedical substance pig kidneys |
| title | Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis |
| title_full | Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis |
| title_fullStr | Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis |
| title_full_unstemmed | Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis |
| title_short | Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis |
| title_sort | experimental testing of the new biomedical substance of pig kidneys for the treatment of nephrolithiasis |
| topic | experimental nephrolithiasis anti-lithogenic activity biomedical substance pig kidneys |
| url | http://ijbm.org/articles/IJBM_7(1)_OA4.pdf |
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