Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was p...
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2017-01-01
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| Online Access: | http://www.mdpi.com/1996-1073/10/1/35 |
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| author | Abdi Hanra Sebayang Masjuki Haji Hassan Hwai Chyuan Ong Surya Dharma Arridina Susan Silitonga Fitranto Kusumo Teuku Meurah Indra Mahlia Aditiya Harjon Bahar |
| author_facet | Abdi Hanra Sebayang Masjuki Haji Hassan Hwai Chyuan Ong Surya Dharma Arridina Susan Silitonga Fitranto Kusumo Teuku Meurah Indra Mahlia Aditiya Harjon Bahar |
| author_sort | Abdi Hanra Sebayang |
| collection | DOAJ |
| description | Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. |
| first_indexed | 2024-04-11T20:43:58Z |
| format | Article |
| id | doaj.art-e8e392af28584563924ff6ebd753f305 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T20:43:58Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-e8e392af28584563924ff6ebd753f3052022-12-22T04:04:07ZengMDPI AGEnergies1996-10732017-01-011013510.3390/en10010035en10010035Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface MethodologyAbdi Hanra Sebayang0Masjuki Haji Hassan1Hwai Chyuan Ong2Surya Dharma3Arridina Susan Silitonga4Fitranto Kusumo5Teuku Meurah Indra Mahlia6Aditiya Harjon Bahar7Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Mechanical Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor, MalaysiaDepartment of Mechanical Engineering, Faculty of Engineering, The University of Melbourne, Parkville, VIC 3010, AustraliaBioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock.http://www.mdpi.com/1996-1073/10/1/35Manihot glaziovii (M. glaziovii)optimizationhydrolysisfermentationbioethanolalternative fuel |
| spellingShingle | Abdi Hanra Sebayang Masjuki Haji Hassan Hwai Chyuan Ong Surya Dharma Arridina Susan Silitonga Fitranto Kusumo Teuku Meurah Indra Mahlia Aditiya Harjon Bahar Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology Energies Manihot glaziovii (M. glaziovii) optimization hydrolysis fermentation bioethanol alternative fuel |
| title | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_full | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_fullStr | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_full_unstemmed | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_short | Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology |
| title_sort | optimization of reducing sugar production from manihot glaziovii starch using response surface methodology |
| topic | Manihot glaziovii (M. glaziovii) optimization hydrolysis fermentation bioethanol alternative fuel |
| url | http://www.mdpi.com/1996-1073/10/1/35 |
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