Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope
A recently developed technique, namely multiple beam interference microscopy, has been applied to investigate the morphology of the parasite Toxoplasma gondii for the first time. The interference pattern obtained from the multiple internal reflection of a T. gondii, sandwiched between a glass plate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-10-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000700017 |
| _version_ | 1797704762406207488 |
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| author | H Medina JM Barboza H Urdaneta M Rondon NV Joshi |
| author_facet | H Medina JM Barboza H Urdaneta M Rondon NV Joshi |
| author_sort | H Medina |
| collection | DOAJ |
| description | A recently developed technique, namely multiple beam interference microscopy, has been applied to investigate the morphology of the parasite Toxoplasma gondii for the first time. The interference pattern obtained from the multiple internal reflection of a T. gondii, sandwiched between a glass plate and a cover plate, was focused on the objective of a conventional microscope. Because of the enhance contrast, several details of sub cellular structure and separating compartments are clearly visible. Details reveal the presence of a nucleus, lipid body, dense granule, rhoptry and amylopectin. The wall thickness of the membrane of the lipid body and the amylopectin is of the order of 0.02 µm and can be clearly distinguished with the help of the present technique. The same parasite has also been examined with the help of atomic force microscopy, and because of its thick membrane, the inner structural details were not observed at all. Sub cellular details of T. gondii observed with the present technique have been reported earlier only by low amplification transmission electron microscopy and not by any optical microscopic technique. |
| first_indexed | 2024-03-12T05:25:14Z |
| format | Article |
| id | doaj.art-e8eae205474745a89553768f340e10bc |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:14Z |
| publishDate | 2001-10-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e8eae205474745a89553768f340e10bc2023-09-03T07:25:03ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-10-0196798398610.1590/S0074-02762001000700017Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscopeH MedinaJM BarbozaH UrdanetaM RondonNV JoshiA recently developed technique, namely multiple beam interference microscopy, has been applied to investigate the morphology of the parasite Toxoplasma gondii for the first time. The interference pattern obtained from the multiple internal reflection of a T. gondii, sandwiched between a glass plate and a cover plate, was focused on the objective of a conventional microscope. Because of the enhance contrast, several details of sub cellular structure and separating compartments are clearly visible. Details reveal the presence of a nucleus, lipid body, dense granule, rhoptry and amylopectin. The wall thickness of the membrane of the lipid body and the amylopectin is of the order of 0.02 µm and can be clearly distinguished with the help of the present technique. The same parasite has also been examined with the help of atomic force microscopy, and because of its thick membrane, the inner structural details were not observed at all. Sub cellular details of T. gondii observed with the present technique have been reported earlier only by low amplification transmission electron microscopy and not by any optical microscopic technique.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000700017multiple beam interference microscopyhigh resolutionmorphologyToxoplasma gondiiatomic force microscopy |
| spellingShingle | H Medina JM Barboza H Urdaneta M Rondon NV Joshi Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope Memorias do Instituto Oswaldo Cruz multiple beam interference microscopy high resolution morphology Toxoplasma gondii atomic force microscopy |
| title | Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope |
| title_full | Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope |
| title_fullStr | Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope |
| title_full_unstemmed | Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope |
| title_short | Morphological investigation of Toxoplasma gondii in vivo by a multiple beam interference microscope |
| title_sort | morphological investigation of toxoplasma gondii in vivo by a multiple beam interference microscope |
| topic | multiple beam interference microscopy high resolution morphology Toxoplasma gondii atomic force microscopy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000700017 |
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