The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma
A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of the fluoroquinolones norfloxacin (NOR), ciprofloxacin (CIP), levofloxacin (LEVO), and moxifloxacin (MOXI) in human plasma has been successfully developed. The detection was optimized with a dual w...
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| Format: | Article |
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Vietnam Ministry of Science and Technology
2022-06-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
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| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/328 |
| _version_ | 1797937799538671616 |
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| author | Diep Thi Minh Nguyen Tuan Anh Vu Dien Xuan Luong Truong Xuan Nguyen |
| author_facet | Diep Thi Minh Nguyen Tuan Anh Vu Dien Xuan Luong Truong Xuan Nguyen |
| author_sort | Diep Thi Minh Nguyen |
| collection | DOAJ |
| description |
A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of the fluoroquinolones norfloxacin (NOR), ciprofloxacin (CIP), levofloxacin (LEVO), and moxifloxacin (MOXI) in human plasma has been successfully developed. The detection was optimized with a dual wavelength excitation and emission fluorescence detector. Analytical wavelengths of λex/λem=290/500 nm (for LEVO and MOXI) and λex/λem=280/445 nm (for NOR and CIP) were found when measuring fluoroquinolones in the mixture. The plasma sample was pre-treated during the deproteinization step using methanol. This method was validated by linearity in the ranges of 0.05-15; 0.05-10; 0.05-15; and 0.10-10 µg ml-1 with limits of detection of 0.0071; 0.0080; 0.0074; and 0.0171 µg ml-1 for NOR, CIP, LEVO, and MOXI, respectively. Further, the HPLC-FLD method was proven to be precise and accurate with relative standard deviations lower than 6% and recoveries ranging from 92.5-105.4% for all four fluoroquinolones. Therefore, the proposed HPLC-FLD method provides an alternative approach for the simultaneous analysis of fluoroquinolones in plasma.A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of the fluoroquinolones norfloxacin (NOR), ciprofloxacin (CIP), levofloxacin (LEVO), and moxifloxacin (MOXI) in human plasma has been successfully developed. The detection was optimized with a dual wavelength excitation and emission fluorescence detector. Analytical wavelengths of λex/λem=290/500 nm (for LEVO and MOXI) and λex/λem=280/445 nm (for NOR and CIP) were found when measuring fluoroquinolones in the mixture. The plasma sample was pre-treated during the deproteinization step using methanol. This method was validated by linearity in the ranges of 0.05-15; 0.05-10; 0.05-15; and 0.10-10 µg ml-1 with limits of detection of 0.0071; 0.0080; 0.0074; and 0.0171 µg ml-1 for NOR, CIP, LEVO, and MOXI, respectively. Further, the HPLC-FLD method was proven to be precise and accurate with relative standard deviations lower than 6% and recoveries ranging from 92.5-105.4% for all four fluoroquinolones. Therefore, the proposed HPLC-FLD method provides an alternative approach for the simultaneous analysis of fluoroquinolones in plasma.
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| first_indexed | 2024-04-10T18:50:52Z |
| format | Article |
| id | doaj.art-e8eb8388c1af4f41856629b81c27599d |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:52Z |
| publishDate | 2022-06-01 |
| publisher | Vietnam Ministry of Science and Technology |
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| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-e8eb8388c1af4f41856629b81c27599d2023-02-01T08:12:41ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-06-0164210.31276/VJSTE.64(2).16-20The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasmaDiep Thi Minh Nguyen0Tuan Anh Vu1Dien Xuan Luong2Truong Xuan Nguyen3School of Chemical Engineering, Hanoi University of Science and TechnologySchool of Chemical Engineering, Hanoi University of Science and TechnologySchool of Chemical Engineering, Hanoi University of Science and TechnologySchool of Chemical Engineering, Hanoi University of Science and Technology A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of the fluoroquinolones norfloxacin (NOR), ciprofloxacin (CIP), levofloxacin (LEVO), and moxifloxacin (MOXI) in human plasma has been successfully developed. The detection was optimized with a dual wavelength excitation and emission fluorescence detector. Analytical wavelengths of λex/λem=290/500 nm (for LEVO and MOXI) and λex/λem=280/445 nm (for NOR and CIP) were found when measuring fluoroquinolones in the mixture. The plasma sample was pre-treated during the deproteinization step using methanol. This method was validated by linearity in the ranges of 0.05-15; 0.05-10; 0.05-15; and 0.10-10 µg ml-1 with limits of detection of 0.0071; 0.0080; 0.0074; and 0.0171 µg ml-1 for NOR, CIP, LEVO, and MOXI, respectively. Further, the HPLC-FLD method was proven to be precise and accurate with relative standard deviations lower than 6% and recoveries ranging from 92.5-105.4% for all four fluoroquinolones. Therefore, the proposed HPLC-FLD method provides an alternative approach for the simultaneous analysis of fluoroquinolones in plasma.A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of the fluoroquinolones norfloxacin (NOR), ciprofloxacin (CIP), levofloxacin (LEVO), and moxifloxacin (MOXI) in human plasma has been successfully developed. The detection was optimized with a dual wavelength excitation and emission fluorescence detector. Analytical wavelengths of λex/λem=290/500 nm (for LEVO and MOXI) and λex/λem=280/445 nm (for NOR and CIP) were found when measuring fluoroquinolones in the mixture. The plasma sample was pre-treated during the deproteinization step using methanol. This method was validated by linearity in the ranges of 0.05-15; 0.05-10; 0.05-15; and 0.10-10 µg ml-1 with limits of detection of 0.0071; 0.0080; 0.0074; and 0.0171 µg ml-1 for NOR, CIP, LEVO, and MOXI, respectively. Further, the HPLC-FLD method was proven to be precise and accurate with relative standard deviations lower than 6% and recoveries ranging from 92.5-105.4% for all four fluoroquinolones. Therefore, the proposed HPLC-FLD method provides an alternative approach for the simultaneous analysis of fluoroquinolones in plasma. https://vietnamscience.vjst.vn/index.php/vjste/article/view/328dual wavelength excitation and emissionfluoroquinolonesHPLC-FLD, plasma |
| spellingShingle | Diep Thi Minh Nguyen Tuan Anh Vu Dien Xuan Luong Truong Xuan Nguyen The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma Vietnam Journal of Science, Technology and Engineering dual wavelength excitation and emission fluoroquinolones HPLC-FLD, plasma |
| title | The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| title_full | The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| title_fullStr | The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| title_full_unstemmed | The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| title_short | The development of HPLC dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| title_sort | development of hplc dual wavelength fluorescence detection method for the simultaneous determination of four fluoroquinolones in human plasma |
| topic | dual wavelength excitation and emission fluoroquinolones HPLC-FLD, plasma |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/328 |
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