Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation
Fungal resistance that leads to the failure of drug therapy due to biofilm development is a major clinical challenge. Various polysaccharides have been used to control biofilm formation by drug-resistant fungi, and this study was undertaken to develop chitosan (CS)-modified materials and evaluate th...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/7/950 |
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| author | Chaitany Jayprakash Raorane Divya Shastri Asrafali Shakila Parveen Rajesh Haldhar Vinit Raj Seong-Cheol Kim |
| author_facet | Chaitany Jayprakash Raorane Divya Shastri Asrafali Shakila Parveen Rajesh Haldhar Vinit Raj Seong-Cheol Kim |
| author_sort | Chaitany Jayprakash Raorane |
| collection | DOAJ |
| description | Fungal resistance that leads to the failure of drug therapy due to biofilm development is a major clinical challenge. Various polysaccharides have been used to control biofilm formation by drug-resistant fungi, and this study was undertaken to develop chitosan (CS)-modified materials and evaluate their abilities to inhibit <i>Candida</i> biofilm growth. CS was grafted with methacrylamide (MA) and acrylonitrile (AN) and, to improve its application characteristics further, was grafted with hyaluronic acid to produce CS-g-poly (MA-co-AN) HA complex. Grafting and complex formation were confirmed using spectroscopic techniques. CS-g-poly (MA-co-AN) HA was tested to investigate its ability to inhibit <i>Candida albicans</i> biofilm formation and showed significant antibiofilm activity at 200 µg/mL. Additionally, CS-g-poly (MA-co-AN) HA did not have any toxic effect on <i>Caenorhabditis elegans</i>. Thus, this study provides an innovative means of preventing microorganism-associated biofilm formation. |
| first_indexed | 2024-03-09T03:46:55Z |
| format | Article |
| id | doaj.art-e8ed6d4a607b42a4afc3c78f8ae3a113 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T03:46:55Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-e8ed6d4a607b42a4afc3c78f8ae3a1132023-12-03T14:32:59ZengMDPI AGAntibiotics2079-63822022-07-0111795010.3390/antibiotics11070950Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm FormationChaitany Jayprakash Raorane0Divya Shastri1Asrafali Shakila Parveen2Rajesh Haldhar3Vinit Raj4Seong-Cheol Kim5School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Pharmacy, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaFungal resistance that leads to the failure of drug therapy due to biofilm development is a major clinical challenge. Various polysaccharides have been used to control biofilm formation by drug-resistant fungi, and this study was undertaken to develop chitosan (CS)-modified materials and evaluate their abilities to inhibit <i>Candida</i> biofilm growth. CS was grafted with methacrylamide (MA) and acrylonitrile (AN) and, to improve its application characteristics further, was grafted with hyaluronic acid to produce CS-g-poly (MA-co-AN) HA complex. Grafting and complex formation were confirmed using spectroscopic techniques. CS-g-poly (MA-co-AN) HA was tested to investigate its ability to inhibit <i>Candida albicans</i> biofilm formation and showed significant antibiofilm activity at 200 µg/mL. Additionally, CS-g-poly (MA-co-AN) HA did not have any toxic effect on <i>Caenorhabditis elegans</i>. Thus, this study provides an innovative means of preventing microorganism-associated biofilm formation.https://www.mdpi.com/2079-6382/11/7/950chitosanhyaluronic acidgraftingcomplexantibiofilm |
| spellingShingle | Chaitany Jayprakash Raorane Divya Shastri Asrafali Shakila Parveen Rajesh Haldhar Vinit Raj Seong-Cheol Kim Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation Antibiotics chitosan hyaluronic acid grafting complex antibiofilm |
| title | Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation |
| title_full | Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation |
| title_fullStr | Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation |
| title_full_unstemmed | Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation |
| title_short | Grafted Chitosan-Hyaluronic Acid (CS-g-poly (MA-co-AN) HA) Complex Inhibits Fluconazole-Resistant <i>Candida albicans</i> Biofilm Formation |
| title_sort | grafted chitosan hyaluronic acid cs g poly ma co an ha complex inhibits fluconazole resistant i candida albicans i biofilm formation |
| topic | chitosan hyaluronic acid grafting complex antibiofilm |
| url | https://www.mdpi.com/2079-6382/11/7/950 |
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